Biology subjects

Man, Y.

Publications and source records attributed to Man, Y..

4 recordsLinked to original sources

bioRxiv2021

The Diameter Factor of Aligned Electrospinning Membranes Facilitates Cutaneous Wound Healing by Promoting Epithelialization

Topographical properties (such as pattern and diameter) of biomaterials play important roles in influencing cell activities and manipulating the related immune response during the skin wound healing. We prepared aligned electrospinning membranes with different fiber diameters including 319{+/-}100 nm (A300), 588{+/-}132 nm (A600) and 1048{+/-}130 nm (A1000). The A300 membranes significantly improved cell proliferation and spreading, and facilitated skin wound healing (epithelization and vascularization) with the regeneration of hair follicles. The transcriptome revealed the underlying molecular mechanism that A300 could promote macrophages transformation to reparative phenotypes with highly expressed IL10 and TGF{beta}, subsequently promoting epidermal cell migration via secreting matrix metalloproteinases 12 (MMP12). All the results indicate the A300 to be a potential candidate for guided skin regeneration applications. Graphic abstract O_FIG O_LINKSMALLFIG WIDTH=200 HEIGHT=95 SRC="FIGDIR/small/432929v3_ufig1.gif" ALT="Figure 1"> View larger version (56K): org.highwire.dtl.DTLVardef@17d754borg.highwire.dtl.DTLVardef@19fcb2forg.highwire.dtl.DTLVardef@1d4bc1eorg.highwire.dtl.DTLVardef@18a8091_HPS_FORMAT_FIGEXP M_FIG C_FIG

bioengineering↗

bioRxiv2020

Application of EGCG modified EDC/NHS cross-linked extracellular matrix to promote macrophage adhesion

Though chemically cross-linked by EDC/NHS endows collagen membrane with promising mechanical properties, it is not conducive to modulation of foreign body reaction (FBR) after implantation or guidance of osteogenesis. In our previous research, we have found that macrophages have a strong regulatory effect on tissue and bone regeneration during FBR, and EGCG modified membranes could adjust the recruitment and phenotypes of macrophages. Accordingly, we develop the EGCG-EDC/NHS membranes, prepared with physically immersion, while the surface morphology of the membrane was observed by SEM, the biological activity of collagen was determined by FTIR, the activity and adhesion of cell culture in vitro, angiogenesis and monocyte/macrophage recruitment after subcutaneous implantation, etc. are characterized. It could be concluded that EGCG-EDC/NHS collagen membrane is hopeful to be used in implant dentistry for it not only retains the advantages of the collagen membrane itself, but also improves cell viability, adhesion and vascularization tendency. However, the mechanism that lies in the regenerative advantages of such membrane needs further exploration, but it is certain that the differences in surface morphology can have a significant impact on the reaction between the host and the implant, not to mention macrophage in bone regeneration.

immunology↗

bioRxiv2020

Consideration of macrophage phenotypes under biomaterials mediated host defense process

Culturing macrophage in vitro is an important means to understand its reaction towards co-culture substances. However, the source of macrophages can be either purchased from specific cell line or extracted from bone marrow as differentiated macrophages. In order to assure the precision, when adopting extracted primary cell, screening in advance will be prominent before any processes to avoid results reserving that of non-macrophages. Here, we perform single-cell sequencing on open wound of skin in mice and focusing on the secreted proteins and surface markers related to traditional macrophage phenotypes (M1/M2) to ensure the importance of screening. The expression of CAMP when macrophages fight against candida albicans is another target to see its relationship with current classification. And results showed that identifying its phenotype without screening macrophages will far from the exact situation, and the expression of CAMP cannot be carried out by the traditional M1 and M2 macrophage. Thus, determining phenotype of macrophages based on function would be a promising way.

immunology↗

bioRxiv2020

ARG1 could be expressed by human, but not represent for repair

Arginase 1 (ARG1), typically expressed under stress or stimulation rather than in the healthy condition in humans. However, more and more studies claim ARG1 could be expressed by human and represents healing process. Including THP-1 monocyte shift to M2 macrophages rendered a higher expression of ARG1 than that with bone-graft has an ideal effect on anti-inflammation, resulting in tissue healing. With the higher expression of ARG1 seemingly induced by the constructed material and represented by the anti-inflammatory situation. For these reasons, this study focuses on whether traditional markers of macrophages could be express by human or not during clinical healing process. Through harvesting bone tissue samples during bone healing process and confirm the expression of markers at transcriptome and protein levels. The results showed the expression of ARG1 relatively low by bulk-RNA sequencing, quantitative polymerase chain reaction (qPCR), immunofluorescence (IF) and flow cytometry or could not be detected during process of healing of healthy donor. Taken together, results indicate that the ARG1 is not chosen for a regenerative marker in human bone tissue.

immunology↗