Biology subjects

Mamaeva, A.

Publications and source records attributed to Mamaeva, A..

5 recordsLinked to original sources

bioRxiv2024

Comparative Analysis of Small Secreted Peptide Signaling during Defense Response: Insights from Vascular and Non-Vascular Plants

Small secreted peptides (SSPs) play an important role in modulating immune responses in all land plants. However, the evolution of stress peptide signaling in different plant phyla remains poorly understood. Here, we compared the expression of SSP genes in the pathogen-induced transcriptomes of vascular and non-vascular plants. We found 13, 19, 15, and 28 SSP families that were differentially expressed during infection in Physcomitrium patens, Zea mays, Brassica napus, and Solanum tuberosum, respectively. A comparative study of peptide motifs and predicted three-dimensional structures confirmed the similarity of SSPs across the examined plant species. In both vascular and non-vascular plants, however, only the RALF peptide family was differentially regulated under infection. We also found that EPFL peptides, which are involved in growth and development processes in angiosperms, were differentially regulated in P. patens in response to pathogen infection. The search for novel immune-specific peptides revealed a family of PSY-like peptides that are differentially regulated during infection in P. patens. The treatment with synthetic EPFL, MEG, PSY, and PSY-like peptides validated their roles in the immune response and growth regulation. Thus, our study showed the complex nature of SSP signaling and shed light on the regulation of SSPs in different plant lineages during infection.

plant biology↗

bioRxiv2022

RALF peptides modulate immune response in the moss Physcomitrium patens

RAPID ALKALINIZATION FACTOR (RALFs) are cysteine-rich peptides that regulate multiple physiological processes in plants. This peptide family has considerably expanded during land plant evolution, but the role of ancient RALFs in modulating stress response is unknown. Here, we used the moss Physcomitrium patens as a model to gain insight into the role of RALF peptides in coordination of plant growth and stress response in non-vascular plants. The quantitative proteomic analysis revealed concerted downregulation of M6 metalloproteases and some membrane proteins, including those involved in stress response, in PpRALF1, 2 and 3 knockout (KO) lines. We found that knockout of PpRALF2 and PpRALF3 genes resulted in increased resistance to bacterial and fungal phytopathogens - Pectobacterium carotovorum and Fusarium solani, suggesting the role of these peptides in negative regulation of immune response in P. patens. The comparative transcriptome analysis of PpRALF3 KO and wild type plants under Fusarium solani infection showed the clear difference in regulation of genes belonging to phenylpropanoid pathway and associated with cell wall modification and biogenesis between these genotypes. The follow-up analysis revealed the role of PpRALF3 in growth regulation under abiotic and biotic stress regulation, which suggests the role of RALFs in responses to different adverse conditions. Thus, our study sheds light on the function of the previously uncharacterized PpRALF3 peptide and gives a clue to ancestral functions of RALF peptides in plant stress response.

plant biology↗

bioRxiv2021

FAMOSS, a conserved 41-aa peptide involved in plant tip growth regulation

Recent evidence shows that small open reading frame (smORF; <100 codons)-encoded peptides (SEPs) containing transmembrane domains are preadapted to be progenitors of novel functional genes. A dozen of such SEPs translated from long non-coding RNAs (lncRNAs) are already functionally characterised in animals. However, functional plant lncRNA-smORF-coded peptides are not yet described. Here, we report detailed functional characterization of a 41-aa peptide encoded by lncRNA-smORFs in the moss Physcomitrium patens, which was named "FAst-growing MOSS" (FAMOSS). We found that the FAMOSS interacts with the Rab-type small GTPase proteins and its overexpression leads to faster moss growth rate and more intensive vesicular transport in apical cells, while its knockout results in the opposite effect. The FAMOSS contains a predicted transmembrane domain and possible orthologs from streptophyta algae to flowering plants have a very conserved structure. Thus, the FAMOSS peptide is a previously unknown conserved player of Rab-mediated processes in plants. Our findings are in line with functional studies of transmembrane SEPs in animals and prove the principles of SEPs evolution. This study provides new insights into functions of plant lncRNA-smORFs.

plant biology↗

bioRxiv2020

ROS in tobacco stigma exudate affect pollen proteome and provoke membrane hyperpolarization

1.ROS are known to be accumulated in stigmas of different species and can possibly perform different functions in plant reproduction. Here we confirm the assumption that they affect pollen by altering ion transport through the plasma membrane; as a more deferred effect, pollen proteome is modified. We detected ROS in stigma exudate, found hyperpolarization in exudate-treated growing pollen tubes and used flow cytometry of pollen protoplasts to compare the effects of fresh exudate and exogenous H2O2 on pollen tube plasmalemma. Exudate causes plasmalemma hyperpolarization similar to the one provoked by H2O2, which is abolished by catalase treatment and ROS quencher MnTMPP. Inhibitory analysis indicates the participation of Ca2+- and K+-conducting channels in the observed hyperpolarization, linking obtained data with previous patch-clamp studies in vitro. For a deeper understanding of pollen response to ROS we analyzed proteome alterations in H2O2-treated pollen grains. We found 50 unique proteins and 20 differently accumulated proteins that are mainly involved in cell metabolism, energetics, protein synthesis and folding. Thus, pollen is getting ready for effective resource usage, construction of cellular components and rapid growth. HighlightsO_LIThe active substance in stigma exudate is H2O2 C_LIO_LIH2O2 causes hyperpolarization mediated by the activation of cation channels. C_LIO_LIH2O2 affects pollen proteome; we found 50 unique proteins. C_LI

plant biology↗

bioRxiv2017

Distinct types of short open reading frames are translated in plant cells

Genomes contain millions of short (<100 codons) open reading frames (sORFs), which are usually dismissed during gene annotation. Nevertheless, peptides encoded by such sORFs can play important biological roles, and their impact on cellular processes has long been underestimated. Here, we analyzed approximately 70,000 transcribed sORFs in the model plant Physcomitrella patens (moss). Several distinct classes of sORFs that differ in terms of their position on transcripts and the level of evolutionary conservation are present in the moss genome. Over 5000 sORFs were conserved in at least one of ten plant species examined. Mass spectrometry analysis of proteomic and peptidomic datasets suggested that 584 sORFs located on distinct parts of mRNAs and long non-coding RNAs (lncRNAs) are translated, including 73 conservative sORFs. Translational analysis of the sORFs and main ORFs at a single locus suggested the existence of genes that code for multiple proteins and peptides with tissue-specific expression. Alternative splicing is likely involved in the excision of translatable sORFs from such transcripts. We identified a group of sORFs homologous to known protein domains and suggested they function as small interfering peptides. Functional analysis of candidate lncRNA-encoded peptides showed it to be involved in regulating growth and differentiation in moss. The high evolutionary rate and wide translation of sORFs suggest that they may provide a reservoir of potentially active peptides and their importance as a raw material for gene evolution. Our results thus open new avenues for discovering novel, biologically active peptides in the plant kingdom.

plant biology↗