Biology subjects

Mahardhika, I. W. S.

Publications and source records attributed to Mahardhika, I. W. S..

2 recordsLinked to original sources

bioRxiv2021

Third Backcross Generation Indonesian Indigenous Chicken Kampong Broiler-Type (Kambro) SOX5 Gene Polymorphism

The comb is an accessory organ on the head of chicken which is influenced by testosterone hormone and can be used as an indicator of chickens fertility. Comb shape is related to climate adaptation and associated with a dominant mutation in chicken chromosome 1. Therefore this research was aimed to study the association between Pea-comb shape and SOX5 gene polymorphism in the population of progenies (BC-III Kambro) derived from a crossbreed between females Pelung and males second backcross generation Kampong Broiler-Type (BC-II Kambro). Chicken (Gallus gallus) SOX5 gene was acquired from NCBI GenBank with the Ref. Seq. 418195. Primers used to amplify the SOX5 gene are (F):5-AGGTAGCCATGGTGACAAGC-3,(R):5-GATCTGTGAGGCAGCCAGTT-3. Progenies showed 100% Pea-comb shape, while parental generation composed of Pea-comb shape and Single-comb shape. PCR-RFLP and endonuclease restriction enzyme HindIII were unable to determine the genotype of the female parent with Pea-comb. The result of SOX5 gene polymorphism showed the comb shape uniformity between progenies and parental of Kambro backcross generation. This study concluded that the genotype of Pea-comb shape female Pelung was undetermined and there was no polymorphism of the SOX5 gene between pea and single comb. PCR-RFLP using endonuclease restriction enzyme HindIII produced both target products and non-target/artifact products. The sequencing procedure was required to provide nucleotide sequences.

genetics↗

bioRxiv2021

Exon 9 LEPR Gene SNP Polymorphism of Hybrid Chickens F2 Kambro Crossbreeds of female F1 Kambro with male F1 Kambro

The implementation of the T-ARMS PCR method in the detection of single nucleotide polymorphisms (SNPs) in the LEPR gene in chicken DNA samples has never been conducted. This research aims to design a specific protocol for exon 9 LEPR gene SNPs detection and detect LEPR gene expression or LEPR SNPs in Pelung chicken samples, F1 Pelung, Layer, Broiler Cobb 500, F1 Kambro chicken and F2 Kambro chicken using the T-ARMS PCR method. Determination of LEPR gene correlation degree on Body Weight (BT) and Egg Productivity (PT) in F1 Kambro population and F2 Kambro. Qualitative phenotype parameters showed six groups of segregated phenotypes compared to F1 Kambro chicken. Growth of F2 Kambro chicken weight reached 753.36 {+/-} 155.31 grams in 8 weeks was not significant for F1 Kambro chicken due to inbreeding depression (Fx = 25%, IR = 4.925%) and transversion of A LEPR allele mutations. Specific protocol detection of exon 9 LEPR gene SNPs using the T-ARMS PCR method can detect C127A LEPR mutations with IP: OP ratio 10:1 pmol / {micro}M, chicken DNA template concentration of 100 ng / {micro}L with annealing temperature of 55.7{degrees} C / 30s. The transversion mutation of C127A of LEPR exon 9 SNP were detected in DNA samples of F1 Kambro hens (80%), F2 Kambro roosters (20%), Broiler Cobb 500 hens (75%). The mutations were not detected in Layer, Pelung Blirik Hitam chicken and F1 Pelung populations.

genetics↗