Luo, J. Q. | bioRxiv Science

bioRxiv2025

Research on the Estimation Model of Seedling Sowing Period Based on Different Altitude

The selection of sowing date is a key link in tobacco planting, and an appropriate sowing date can affect the survival rate and agronomic traits of tobacco plants. This study collected data on tobacco seedlings during the sowing and transplanting periods in the southwestern region of China, combined with altitude gradient data of the planting area. Time series analysis was used to construct a tobacco seedling sowing period estimation model and establish an application platform. The model was validated by comparing the survival rate of transplanting. The results indicate that the proposed LSTM model has an R2 of 0.893, a MAE of 1.796, an RSME of 2.221, and a validation model accuracy of 93.3%. The research results provide scientific sowing time basis for tobacco growers in different altitude regions, and results provide scientific sowing time basis for tobacco growers in different altitude regions, and offer suggestions for optimizing tobacco planting management and promoting the sustainable development of the tobacco industry.

ecology↗

bioRxiv2025

Opposing effects of H2A.Z on memory, transcription and pathology in male and female Alzheimer's disease mice and patients

Alzheimers disease (AD) is a devastating neurodegenerative disease that disproportionately impacts women, but underlying mechanisms for sex-divergent outcomes are unknown. Here, we show that the histone variant H2A.Z is a novel sex-specific regulator of AD in human patients and AD model mice. Specifically, H2A.Z binding in chromatin declines in female and increases in male AD patients, indicating opposite patterns of AD-related H2A.Z dysregulation each sex. These sex differences were recapitulated in the 5xFAD model of AD, in which females accumulated H2A.Z at early disease stages and lost H2A.Z as the disease progressed, suggesting that H2A.Z occupancy shifts with advancing disease. Males showed no change in H2A.Z binding in early disease, but exhibited increased binding as disease progressed, albeit to a lesser extent than females. Consistent with sex-specific H2A.Z dysregulation, H2A.Z depletion produced sex-specific changes in gene expression, whereby H2A.Z was more repressive in female than in male mice and in 5xFAD than in WT males, suggesting that H2A.Zs role in transcription varies with sex and disease. Moreover, H2A.Z depletion improved memory and AD pathology in females, while impairing memory and worsening pathology in male mice. Together, these data suggest that H2A.Z is protective in males and detrimental in females with AD, with key implications for sex-specific therapeutic targeting of chromatin factors.

neuroscience↗