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Lovric, M.

Publications and source records attributed to Lovric, M..

4 recordsLinked to original sources

Agreement between Garmin and Ultrahuman wearables and their association with continuous glucose monitoring: an n-of-1 study

Background: Consumer wearables and continuous glucose monitors (CGM) are increasingly worn together outside clinical settings. Few studies have examined whether two wearables worn at the same time agree with each other, and whether either tracks glucose metrics derived from CGM. Objective: In one adult under free-living conditions, we examined (1) how well sleep, heart rate, heart rate variability (HRV) and activity metrics from a Garmin smartwatch and an Ultrahuman ring agreed with each other, and (2) how these metrics related to glucose measured by CGM. Methods: One healthy adult with no diagnosed diabetes wore a Garmin Fenix 8 watch on the wrist, an Ultrahuman Ring AIR and an Abbott FreeStyle Libre CGM at the same time for 12 days (29 July to 9 August 2026; 1101 glucose readings). We assessed agreement between the devices with Pearson correlation and Bland-Altman analysis (mean bias and 95% limits of agreement, LoA) for daily metrics and for heart rate within the day, and with Cohen's kappa for sleep stages, minute by minute. We related the wearable metrics to mean glucose, glucose variability (coefficient of variation, CV%) and time in range (TIR, 3.9 to 10.0 mmol/L), reading by reading (over the whole record and separately for sleep and waking hours) and day by day. All correlations are descriptive. Because the Ultrahuman ring recorded no data for 22 hours on 3 August and its daytime heart rate behaved differently afterwards, heart-rate analyses are reported for the whole period and separately before and after that gap. The daily results were checked by leaving out one day at a time and by excluding the first 24 hours of sensor wear. Results: The devices agreed closely on total sleep duration (r=0.94; bias -0.02 h, 95% LoA -0.56 to 0.51 h). Daily steps correlated strongly (r=0.99 over the 11 days with data from both devices), but Garmin counted more steps on every day (mean 11,157 vs. 8,929 steps per day; bias +2228 steps). Heart rate agreed moderately during sleep (r=0.68). While awake, agreement was moderate before the gap (r=0.54) and absent after it (r=0.08), when Ultrahuman's daytime heart rate stayed near 70 bpm. The devices assigned the same sleep stage in 50.0% of 4525 minutes (kappa=0.28). Mean glucose was 5.13 mmol/L (SD 0.82) and TIR was 97.5%. Of all wearable signals, heart rate was most closely associated with glucose (Garmin r=0.43 over all readings), but the correlation fell to 0.34 within sleep and 0.21 within waking hours, and before the gap the two devices' waking correlations were alike (Garmin 0.25, Ultrahuman 0.24). Across the 12 days, correlations between night-time wearable metrics and glucose variability weakened markedly when the first 24 hours of sensor wear were excluded (absolute r fell from 0.59 or more to 0.34 or less), and TIR was 100% on 8 of the 12 days, which left it little room to vary. Conclusions: The two wearables agreed on how long the participant slept but not on how the night was staged, and their heart-rate agreement depended on whether the participant was asleep or awake and on the period of wear. Of all wearable signals, heart rate was most closely associated with glucose, mainly because both are lower at night and higher during the day. The associations across days rested largely on the first night of sensor wear. In this single participant, the devices behaved differently by device and by period of wear, so studies that combine different wearables, or relate them to CGM, should check for such differences before pooling data.

physiology↗

Metabolomic fingerprints of PAH exposure - identifying toxicological biomarkers in dynamically cultured 3D cell spheroids

Environmental exposure to polycyclic aromatic hydrocarbons (PAHs) causes metabolic dysfunction, but reliable biomarkers are still needed to assess human health effects. This study used 21-day matured human HepG2 spheroids, a metabolically competent three-dimensional (3D) liver model, to assess metabolic responses to graded, non-cytotoxic concentrations of benzo[a]pyrene (BaP) and benzo[b]fluoranthene (BBF) after 24- and 96-h exposure. Untargeted liquid chromatography-mass spectrometry (LC-MS) metabolomics, combined with multivariate and network analyses, identified compound- and time-specific metabolic signatures. At 24 hours, no metabolites showed significant changes. In contrast, at 96 hours, both PAHs consistently altered seven robust metabolites linked to polyamine metabolism, membrane dynamics, mitochondrial energy, and DNA-repair pathways. Network analysis showed BBF caused broader and more connected changes than BaP, indicating distinct toxicodynamics. These findings underscore the importance of extended exposure in revealing metabolic disruption and support a set of candidate biomarkers for future low-dose studies and improved risk assessment of airborne toxicants.

systems biology↗

A chemical structure and machine learning approach to assess the potential bioactivity of endogenous metabolites and their association with early-childhood hs-CRP levels

Metabolomics has gained much attraction due to its potential to reveal molecular disease mechanisms and present viable biomarkers. In this work we used a panel of untargeted serum metabolomes in 602 childhood patients of the COPSAC2010 mother-child cohort. The annotated part of the metabolome consists of 493 chemical compounds curated using automated procedures. Using predicted quantitative-structure-bioactivity relationships for the Tox21 database on nuclear receptors and stress response in cell lines, we created a filtering method for the vast number of quantified metabolites. The metabolites measured in childrens serums used here have predicted potential against the chosen target modelled targets. The targets from Tox21 have been used with quantitative structure-activity relationships (QSARs) and were trained for [~]7000 structures, saved as models, and then applied to 493 metabolites to predict their potential bioactivities. The models were selected based on strict accuracy criteria surpassing random effects. After application, 52 metabolites showed potential bioactivity based on structural similarity with known active compounds from the Tox21 set. The filtered compounds were subsequently used and weighted by their bioactive potential to show an association with early childhood hs-CRP levels at six months in a linear model supporting a physiological adverse effect on systemic low-grade inflammation. The significant metabolites were reported. O_FIG O_LINKSMALLFIG WIDTH=200 HEIGHT=103 SRC="FIGDIR/small/567095v1_ufig1.gif" ALT="Figure 1"> View larger version (34K): org.highwire.dtl.DTLVardef@9c0216org.highwire.dtl.DTLVardef@4d26fborg.highwire.dtl.DTLVardef@13a2f0corg.highwire.dtl.DTLVardef@e6cbd1_HPS_FORMAT_FIGEXP M_FIG C_FIG

bioinformatics↗

Predicting Morphological Disparities In Sea Urchin Skeleton Growth And Form

Sea urchins exhibit among their many species remarkable diversity in skeleton form (e.g., from spheroid to discoid shapes). However, we still do not understand how some related species show distinct morphologies despite inherent similarities at the genetic level. For this, we use theoretical morphology to disentangle the ontogenic processes that play a role in skeletal growth and form. We developed a model that simulates these processes involved and predicted trajectory obtaining 94% and 77% accuracies. We then use the model to understand how morphologies evolved by exploring the individual effects of three structures (ambulacral column, plate number, and polar regions). These structures have changed over evolutionary time and trends indicate they may influence skeleton shape, specifically height-to-diameter ratio, h:d. Our simulations confirm the trend observed but also show how changes in the attributes affect shape; we show that widening the ambulacral column or increasing plate number in columns produces a decrease in h:d (flattening); whereas increasing apical system radius to column length ratio produces an increase in h:d (gloublar shape). Computer simulated h:d matched h:d measured from real specimens. Our findings provide the first explanation of how small changes in these structures can create the diversity in skeletal morphologies.

evolutionary biology↗