Biology subjects

Lopez-Granero, C.

Publications and source records attributed to Lopez-Granero, C..

3 recordsLinked to original sources

bioRxiv2026

Understanding the neurocognitive impact of outdoor PM10 and PM2.5 exposure: an in silico dosimetric modeling study using MPPD

Air pollution has been increasingly linked to adverse neurodevelopmental and neurodegenerative outcomes. While experimental and preclinical studies suggest that exposure to particulate matter (PM), particularly during gestation, may disrupt cognitive development, the impact of short-term PM exposure on cognitive and behavioral functioning in healthy young populations remains insufficiently explored in Spain. Moreover, few studies have incorporated individualized dosimetry models to estimate exposure more accurately. This study included 186 healthy young adults (mean age = 20.4 years) recruited from three Spanish cities (Teruel, Almeria, and Talavera) characterized by different pollution levels. Ambient fine and coarse PM concentrations were recorded 8, 15, and 30 days prior to psychological assessment. Instead of relying solely on raw in situ environmental measurements, individualized PM deposition was estimated using the Multiple-Path Particle Dosimetry Model (MPPD), allowing a more biologically meaningful exposure approximation. Psychological outcomes were assessed using validated questionnaires: DASS-21 (depression, anxiety, stress), BIS-11 (impulsivity), UCLA Loneliness Scale, and SWLS (life satisfaction). Behavioral performance was evaluated using computerized versions of the Attentional Network Task (ANT) and the Stroop Task. Blood NRF2 concentrations were analyzed as a biomarker potentially related to oxidative stress mechanisms. In situ data indicated that Talavera presented the highest pollution levels, followed by Almeria and Teruel. Linear regression analyses showed that coarse PM exposure across 8-, 15-, and 30-day windows significantly predicted poorer Executive Control Index performance in the ANT. Additionally, 15-day coarse PM and 30-day fine PM exposure were associated with greater cognitive interference. Oxidative stress markers were significantly associated with PM exposure levels. These findings support emerging evidence that short-term PM exposure may negatively affect executive and attentional processes even in healthy young adults. Further longitudinal research incorporating individualized exposure modeling is warranted to clarify causal pathways and underlying biological mechanisms. Graphical Abstract O_FIG O_LINKSMALLFIG WIDTH=200 HEIGHT=97 SRC="FIGDIR/small/713644v1_ufig1.gif" ALT="Figure 1"> View larger version (79K): org.highwire.dtl.DTLVardef@1a0ac13org.highwire.dtl.DTLVardef@1812accorg.highwire.dtl.DTLVardef@120bf07org.highwire.dtl.DTLVardef@dd9a7c_HPS_FORMAT_FIGEXP M_FIG C_FIG

neuroscience↗

bioRxiv2025

Multi-omics integrative approach of senescent endothelial cells and derived extracellular vesicles in a replicative senescence model

Aging is a major unmodifiable risk factor for cardiovascular disease (CVD). Replicative endothelial senescence (RES), characterized by permanent cell-cycle arrest and a senescence-associated secretory phenotype (SASP), is a hallmark of vascular aging. However, the molecular mechanisms underlying RES, particularly the role of extracellular vesicles (EVs), remain poorly understood. To this aim, an integrated multi-omics approach (proteomics, mRNA, and miRNA profiling) was applied to characterize senescent and early-passage human umbilical vein endothelial cells (HUVECs) and their secreted EVs. Senescent HUVECs and EVs displayed a canonical senescence profile, including cell-cycle arrest, DDR activation, NF-{kappa}B signaling, and SASP induction, alongside marked suppression of RNA metabolism, ribosome biogenesis, and DNA repair. Multi-omics integration has linked endothelial senescence to vascular aging, maladaptive angiogenesis, and ECM remodeling, which are key processes in CVD development. Moreover, senescent EVs propagate senescence by exporting fewer reparative and antioxidant factors while carrying pro-fibrotic, adipogenic, and angiogenic mediators. Multi-omics profiling revealed consistent transcript-protein changes in senescent cells and EVs, defining a robust molecular signature of RES, including endothelial cell-specific markers and post-transcriptional regulators (lncRNAs and miRNAs). Among these, miR-22-3p and miR-126-5p emerged as key modulators in both cells and EVs, with miR-22-3p demonstrating compartment-specific regulation. Integration further uncovered a coordinated regulatory network involving miRNAs (miR-23, miR-335-3p, miR-29 family, miR-590-3p, and miR-126-5p) that were inversely correlated with transcription factors (e.g., REST, KLFs, and ZNFs) and downstream targets, collectively driving endothelial senescence through impaired PI3K-Akt signaling. Although further validation is needed, these findings provide new insights into the molecular mechanisms of RES and open perspectives for modulating secondary senescence and age-related vascular diseases.

cell biology↗

bioRxiv2024

Is Probiotic Supplementation During Pregnancy Safe for Healthy Populations? A Systematic Review of Preclinical Studies

Gut-brain axis and probiotics supplementation during gestational period have gained an especial attention in the field of neurodevelopmental disorders. However, the influence of prenatal probiotics in physiology and neurodevelopment of healthy organisms is widely unexplored and probiotics are commercially such as dietetic supplementations without the necessity of ensuring safety in healthy organisms, as is required for drugs. The aim is to present and discuss the various biochemical, developmental and behavioral effects that prenatal probiotics have on the offspring of healthy organisms in rodent models and assess their security during gestation. We included a total of 15 preclinical studies discarding all those that did not have an appropriate control for the probiotic-treated group. Results suggest that probiotics, particularly Lactobacillus strains, influenced anxiety-like behaviors in mice and GABAergic system maturation, showing an apparent biphasic effect, reducing GABA functioning in early life but increasing them in later stages; and both prenatal Lactobacillus and Bifidobacterium, influenced microbiota and general development of healthy organisms. However, the significant variations in methodologies used across studies complicate the ability to elucidate specific conclusions related to bacterial strains or doses. Further research is imperative to ensure the safety and regulation of probiotic interventions, particularly during the gestational period.

developmental biology↗