Biology subjects

Lipomi, D. J.

Publications and source records attributed to Lipomi, D. J..

4 recordsLinked to original sources

bioRxiv2025

PIEZO channels link mechanical forces to uterine contractions in parturition

AbstractMechanical forces are extensively involved in pregnancy and parturition, but their precise roles and mechanisms remain poorly understood. Here, we identify mechanically activated ion channels PIEZO1 and PIEZO2 as key mechanotransducers required for labor progression. Genetic deletion of Piezo1 and Piezo2 in mice resulted in weakened uterine contractions and severe parturition defects. Tissue-specific knockouts revealed that deletion in either the uterus or sensory neurons alone caused modest defects, whereas combined loss significantly impaired labor, demonstrating additive effects. Single-nuclei sequencing showed that loss of PIEZO reduced expression of connexin43 (Gja1), a gap junction protein in uterine smooth muscle cells, suggesting a mechanistic link to impaired contraction. These findings highlight the critical role of PIEZO channels in mechanotransduction during parturition and suggest therapeutic targets for labor dysfunction.

physiology↗

bioRxiv2024

A key role of PIEZO2 mechanosensitive ion channel in adipose sensory innervation

Compared to the well-established functions of sympathetic innervation, the role of sensory afferents in adipose tissues remains less understood. Recent work revealed the anatomical and physiological significance of adipose sensory innervation; however, its molecular underpinning remains unclear. Here, using organ-targeted single-cell RNA sequencing, we identified the mechanoreceptor PIEZO2 as one of the most prevalent receptors in fat-innervating dorsal root ganglia (DRG) neurons. We found that selective PIEZO2 deletion in fat-innervating neurons phenocopied the molecular alternations in adipose tissue caused by DRG ablation. Conversely, a gain-of-function PIEZO2 mutant shifted the adipose phenotypes in the opposite direction. These results indicate that PIEZO2 plays a major role in the sensory regulation of adipose tissues. This discovery opens new avenues for exploring mechanosensation in organs not traditionally considered mechanically active, such as the adipose tissues, and therefore sheds light on the broader significance of mechanosensation in regulating organ function and homeostasis.

neuroscience↗

bioRxiv2024

Decoupling Coldness and Softness in Tactile Wetness Perception Using Tunable Hydrogels

This study investigates the perception of tactile wetness, a complex sensation experienced by humans. Previous research has primarily focused on either thermal or mechanical cues separately, or has used textiles as stimuli whose parameters are difficult to control. Here, we employed polyacrylamide hydrogels with varying stiffness levels soaked in liquids of distinct thermal conductivities. By psychophysically evaluating participants perception of wetness, we showed that the wetness judgments for the samples exhibit a transitive relationship based on the mechanical and thermal cues from an intrinsically tunable organic material. We developed a prediction model of human wetness judgment with an accuracy of 90% and found that the best metrics for the most accurate model were those that were the most human-adjacent: change in temperature at the skin-sample interface (thermal) and compressive force from 2 mm indentation of the sample (mechanical). Given these parameters, we developed a perceptual space capable of recreating 7 distinct levels of wetness perception with the physical parameters used in this study. The results provide insights into the relative contributions of mechanical and thermal stimulus properties in wetness perception. Most notably, this work highlights that the physical characteristics of the skin-stimulus interface can provide ample information for creating a wetness perceptual space, as opposed to the chemical composition of the hydrogels.

neuroscience↗

bioRxiv2023

Use of dye sensitizers for increased photoacoustic mechanosensation

The photoacoustic effect refers to the generation of pressure waves in matter stimulated by light[1]. In the context of radiology (i.e., photoacoustic imaging) waves generated by pulsed laser light are detected by an ultrasound transducer[2-4]. It has been shown that photoacoustic waves produce a mechanical, tactile sensation in humans on bare skin[5]. In a series of psychophysical experiments, performed with both medical grade and off-the-shelf pulsed light systems, participants could detect, categorically describe, and discern the direction of travel of pulsed optical stimuli with the use of a dye as an optical absorber on the skin. To a large extent, the sensations were perceived as localized vibration on the glabrous surface of the fingers, when sensitized with the thin film of dye. This form of sensory stimulation demonstrates an enhanced non-contact, non-optogenetic, in situ activation of the mechanosensory system. This modality of sensation may provide a tool that leads to new insights in psychology, neuroscience, mechanobiology, and the health sciences. Finally, it has many advantageous characteristics for human interaction with artificial environments, as optical signals can be projected onto the skin across distances.

neuroscience↗