Biology subjects

Lingwan, M.

Publications and source records attributed to Lingwan, M..

4 recordsLinked to original sources

bioRxiv2024

Time course metabolomics and 13CO2 mapping establish BBX31/miP1b mediated metabolic readjustments conferring UV-B tolerance in Arabidopsis

B-box proteins (BBXs) are transcription factors that act as signal transducers in light signaling pathways. The microprotein BBX31/miP1b is known to play a positive role in promoting photomorphogenesis and stress tolerance under UV-B. However, the BBX31-mediated metabolic reprogramming to confer UV-B tolerance in plants is not well characterised. Here, we integrate metabolomics with kinetic 13CO2 tracer-based metabolic mapping, morpho-physiological and biochemical analysis to determine the metabolic rewiring in the UV tolerant genotypes. Our results suggest that BBX31 modulates the levels of photosynthetic compounds, reduces TCA cycle intermediates and enhances GS/GOGAT metabolic intermediates and secondary metabolic pathways. 13CO2 tracing studies established BBX31 modulates phenylpropanoid and GS/GOGAT pathways to divert flux towards the accumulation of UV-B protective metabolites phenylalanine, oxoproline, glutamine, and others. Although metabolomics indicated a higher accumulation of branch chain amino acids (BCAAs) under UV-B, they had negligible 13C incorporation, indicating their biosynthesis from pre-existing intermediates or via protein degradation. Further, we demonstrate that the exogenous application of phenylalanine, identified as one of the marker metabolites, confers tolerance to plants under UV-B. This study sheds light on BBX31-mediated metabolic rewiring under UV-B, which can assist targeted enrichment of metabolites and metabolic engineering to promote UV-B tolerance in plants. HighlightBBX31/miP1b modulates the levels of photosynthetic compounds, reduces TCA cycle intermediates and enhances GS/GOGAT metabolic intermediates to confer UV-B tolerance.

plant biology↗

bioRxiv2023

Mass spectrometry and NMR spectroscopy profiles of red and pink Rhododendron flower petals establish them as rich sources of bioactive secondary metabolites

Rhododendron petals are considered high-value owing to their commercial utility, national/state flower status in certain countries, and bioactive potential from recent studies. Profiling and quantitative analysis of the bioactive metabolites would evaluate if they can be natural sources. This study is focused on comprehensive profiling of secondary metabolites in the petals of Red and Pink Rhododendron flowers (R. arboreum and R. campanulatum) using Mass Spectrometry (GC-MS, LC-MS/MS) and Proton-Nuclear Magnetic Resonance (1H-NMR) Spectroscopy. The profiling highlighted the presence of secondary metabolites belonging to phenolic acids and flavonoids. Specifically, the flowers are rich in promising bioactive molecules such as quinic acid, chlorogenic acid (3-O-caffeoyl quinic acid), protocatechuic acid, coumaroyl quinic acids, catechin, epigallocatechin, and shikimic acid. The profiles are correlated with the metabolic pathways which reflected the activity of shikimic acid, phenolic acid and flavonoid biosynthetic pathways. These metabolites are well reported for their bioactive potential as anti-oxidative, anti-viral, anti-cancerous, anti-diabetic, anti-inflammatory, etc. While the quantitative and multivariate analysis showed variations in the levels of phenolic acids and flavonoids, it is established that red and pink Rhododendron flower petals are a rich source of bioactive phytochemicals of interest to the phytochemical Industry.

plant biology↗

bioRxiv2023

Metabolic, Biochemical, Mineral and Fatty acid profiles of edible Brassicaceae microgreens establish them as promising functional food

Hidden hunger due to micronutrient deficiencies affecting one in three people is a global concern. Identifying functional foods which provide vital health beneficial components in addition to the nutrients is of immense health relevance. Microgreens are edible seedlings enriched with concentrated minerals and phytochemicals whose dietary potential as functional foods needs evaluation. In this study, comprehensive biochemical, mineral, metabolic, and fatty acid profiles of four Brassicaceae microgreens - mustard (Brassica juncea), pak choi (Brassica rapa subsp. chinensis), radish pink (Raphanus sativus), and radish white (Raphanus ruphanistrum) was investigated. The biochemical and mineral profiling confirmed their promising nutritional and antioxidant nature and as excellent sources of minerals. Mineral profiling using inductively coupled plasma mass spectrometry (ICP-MS) exhibited promising levels of Fe, Mn, Mg, K, and Ca in microgreens. Gas chromatography-mass spectrometry (GC-MS) based metabolite profiling highlighted a range of phytochemicals-sugars, amino acids, organic acids, amines, fatty acids, phenol, and other molecules. Fatty acid profiling established promising levels of Oleic acid (C18:1; Monounsaturated fatty acids-MUFA) and linoleic acids (C18:2; omega-6 Poly unsaturated fatty acid-PUFA), which are health beneficial. It is estimated that fresh microgreens (100 g) can meet about 20 % to 50 % recommended dietary allowance (RDA) of macro- and micro-minerals along with providing useful fatty acids and antioxidants. Overall, the study highlighted Brassicaceae microgreens as an excellent nutrient source that can act as functional foods with promising potential to overcome "hidden hunger". Graphical Abstract O_FIG O_LINKSMALLFIG WIDTH=200 HEIGHT=133 SRC="FIGDIR/small/541100v1_ufig1.gif" ALT="Figure 1"> View larger version (62K): org.highwire.dtl.DTLVardef@d3b9c9org.highwire.dtl.DTLVardef@4a7604org.highwire.dtl.DTLVardef@8a164corg.highwire.dtl.DTLVardef@1be187b_HPS_FORMAT_FIGEXP M_FIG C_FIG HighlightsO_LIBrassicaceae microgreens are rich in molecules with relevance to nutrition and health C_LIO_LIThe biochemical analysis supported the antioxidant nature of microgreens C_LIO_LIComprehensive metabolite profiles of edible microgreens of Brassica juncea (Mustard), Brassica rapa subsp. chinensis (Pak Choi), Raphanus sativus (Radish Pink), and Raphanus ruphanistrum (Radish white) using GC-MS are reported C_LIO_LIIonomics analysis using the Brassicaceae microgreens exhibited promising levels of microminerals Fe, Mn, Mg, K, and Ca C_LIO_LIFatty acid profiles show promising levels of Linoleic acid and Oleic acid, which have health relevance C_LI

plant biology↗

bioRxiv2021

A robust method of extraction and GC-MS analysis of Monophenols exhibited UV-B mediated accumulation in Arabidopsis

Studies on specialised metabolites like phenolics are of immense interest owing to their significance to agriculture, nutrition and health. In plants, phenolics accumulate and exhibits spatial and temporal regulations in response to growth conditions. Robust methodologies aimed at efficient extraction of plant phenolics, their qualitative and quantitative analysis is desired. We optimised the analytical and experimental bottlenecks that captured free, ester, glycoside and wall-bound phenolics after acid or alkali treatments of the tissue extracts and subsequent GC-MS analysis. Higher recovery of phenolics from the methanolic extracts was achieved by through a) Ultrasonication assisted extraction along with Methyl tert-butyl ether (MTBE) enrichment b) nitrogen gas drying and c) their derivatisation using MSTFA for GC-MS analysis. The optimised protocol was tested on Arabidopsis rosette exposed to UV-B radiation (280-315 nm) which triggered enhanced levels of 11 monophenols and might be attributed to photoprotection and other physiological roles. Interestingly, coumaric acid (308 m/z) and caffeic acid (396 m/z) levels were enhanced by 12-14 folds under UV-B. Other phenolics such as cinnamic acid (220 m/z), hydroxybenzoic acid (282 m/z), vanillic acid (312 m/z, gallic acid (458 m/z), ferulic acid (338 m/z), benzoic acid (194 m/z), hydroxycinnamic acid (368 m/z) and protocatechuic acid (370 m/z) also showed elevated levels by about 1 to 4 folds. Notably, vanillin (253 m/z) was detected only in the UV-B exposed tissues. The protocol also comprehensively captured the variations in the levels of ester, glycoside and wall-bounded phenolics with high reproducibility and sensitivity. The robust method of extraction and GC-MS analysis can readily be adopted for studying phenolics in plant systems.

plant biology↗