Biology subjects

Lee, D.-G.

Publications and source records attributed to Lee, D.-G..

2 recordsLinked to original sources

bioRxiv2026

Skin-Related Effects of Corynebacterium amycolatum SB-1 Ferment Filtrate and Functional Characterization of Ellagic Acid

Previous research has established a link between the genus Corynebacterium in the skin microbiota and skin health; however, studies investigating the specific effects of Corynebacterium-derived substances on skin cells remain limited. In this study, we isolated eight strains of Corynebacterium from the facial skin of female participant. To investigate the potential skin improvement effects of these isolates, three cultured skin cell lines (Hs68, HaCaT, and B16-F10) were treated with the supernatants of each strain. We then evaluated and compared the gene expression levels (ELN, COL1A1, IL-1{beta}, and HAS3) alongside their inhibitory effects on melanin biosynthesis. Corynebacterium amycolatum SB-1 was selected as the final candidate for subsequent analysis, primarily based on its rapid growth rate and high cell yield. Using LC-TOF/MS, we screened the postbiotic candidates from the SB-1 strain and identified ellagic acid as a known polyphenolic antioxidant with potent anti-melanogenic effects beneficial for skin improvement. To validate the effects of ellagic acid on skin cells, we assessed FLG gene expression and its inhibition of melanin biosynthesis. Additionally, RNA sequencing was performed to elucidate the underlying molecular mechanisms, revealing that ellagic acid is significantly involved in inhibiting melanogenesis and promoting collagen synthesis. Taken together, our results highlight ellagic acid derived from strain SB-1 as a highly effective bioactive compound for improving skin health, specifically targeting hyperpigmentation and skin aging.

genomics↗

bioRxiv2023

A Glance into the Density of Transcriptomic Activity, Embodied by the HOX Genes, in Neonatal and Aging Dermal Cells

Skin is an organ having a crucial role in the protection of muscle, bone, and internal organs and undergoing continuous self-renewal and aged. The growing interest in the prevention of skin aging and rejuvenation has sparked a surge of industrial and research studies focusing on the biological and transcriptional changes that occur during skin development and aging. In this study, we aimed to identify transcriptional differences between two main types of human skin cells: the HDFs and the HEK isolated from 30 neonatal and 30 adults (old) skin. Through differentially expressed gene (DEG) profiling using DEseq2, 604 up-, and 769 down-regulated genes were identified in the old group. The functional classification analysis using Metascape Gene Ontology and Reactome pathway was performed. We report the systematic transcriptomic changes in key biological markers involved in skin formation and maintenance and a unique difference in HOX gene families which are important for developing embryonic formation and regulating numerous biological processes. Among the 39 human HOX genes, 10 genes (HOXA10, 11, 13, HOXB13, HOXC11, and HOXD9-13) were significantly down-regulated, and 25 genes HOXA2-7, HOXB1-9, HOXC4-6 and 8-9, and HOXD1,3,4 and 8) were up-regulated, especially in the old HDFs. We have successfully established a correlation between HOX genes and the process of skin aging, thereby proposing HOX genes as a novel marker for assessing skin aging. Our findings provide compelling evidence supporting the involvement of HOX genes in this biological phenomenon such as skin aging.

genomics↗