Biology subjects

Lavania, M.

Publications and source records attributed to Lavania, M..

2 recordsLinked to original sources

bioRxiv2024

First Detection of Recombinant Enterovirus G Carrying a Torovirus Papain-like cysteine protease Gene from India

Enterovirus G (EV-G) comprising 20 genotypes (G1-G20) are associated with in a various disease conditions in pig populations. The recombination between homologous EV-G is a driving force behind evolution but the recombination with virus from heterologous family is rare event. Presently, two types of recombinant EV-G strains containing torovirus papain like cysteine protease (PLCP) at 2C-3A junction (Type I) and in place of structural proteins (Type II) are reported. The present study conducted to reveal RNA virome in diarrheic piglets faeces retrieved genome coding for complete polyprotein of EV-G1. The detected EV-G1 strain represents first type I recombinant from India. The polyprotein of strain exhibiting 97% amino acid identity was closely related with Chinese strain. Furthermore, the EV-G1-PLCP strain was found to be originated from South Korean, Chinese, and Japanese strains with breakpoints at nucleotide (nt) position 1323 and 4725. Genetic and phylogenetic analysis of VP1 and PLCP of study strain demonstrated its closeness to EV-G1-PLCP strains from South Korea KOR/KNU-1811/2018/G1-PLCP. The present study advances the knowledge about genetic evolution of EV-G from gaining virulence gene from different virus family.

microbiology↗

bioRxiv2023

Detection and Molecular Characterization of Animal Adenovirus and Astrovirus from Western Maharashtra, India

BackgroundAstroviruses (AstV) and adenoviruses (AdV) are associated with diarrhoea in young ones of animals. However, the epidemiology and genetic diversity of AstVs and AdVs in animals are not well studied. Hence the present study was conducted to detect and characterize AstVs and AdVs in calves, piglets and puppies in India. FindingsOut of the processed porcine (48), canine (80), and bovine (65) faecal samples from Western Maharashtra, India, the porcine AstV (PAstV), bovine AstV (BAstV), canine AstV (CAstV), and porcine AdV (PAdV) were detected in 12.5%, 7.69%, 3.75% and 4.1% of samples, respectively. In the RNA-dependent RNA polymerase region-based phylogenetic analysis the detected BAstV strains grouped with MAstV-28, MAstV-33, and MAstV-35, CAstV strains belonged to MAstV-5, PAstV strains belonged to MAstV-24, MAstV-26, and MAstV-31. Both the detected PAdV were of genotype 3, exhibiting 91.9-92.5% nucleotide identity with Ivoirian and Chinese strains. The study reports first-time BAstVs from calves and PAdV-3 from piglets in India. ConclusionThe study revealed diversity in the circulation of AstVs and AdVs in young ones of dogs, pigs and calves. Therefore, extensive epidemiological investigations of AstV and AdV are necessary to confirm their association with diarrhoea and to develop diagnostic tools and control measures.

microbiology↗