Lau, W. Y. V. | bioRxiv Science

bioRxiv2026

Atypical BlaIR Two-Component System in Pseudomonas aeruginosa Regulates Virulence but not β-Lactam Resistance

With the rise of antimicrobial resistance, anti-virulence therapeutics are a viable alternative to circumvent resistance pressures. Hypothetical genes and proteins are an under-studied source of potential virulence factor targets. We performed bioinformatic analyses to identify conserved hypothetical genes enriched in pathogenic Pseudomonas aeruginosa but not in non-pathogenic strains. This analysis identified an atypical BlaIR system, which we named pvmSR, that regulated P. aeruginosa virulence in a Caenorhabditis elegans infection model. This is in contrast with the typical BlaIR system from Staphylococcus aureus, which regulates resistance to {beta}-lac-tam antibiotics. The{Delta} pvmSR mutant showed reduced virulence in a C. elegans slow-killing assay. To understand how PvmSR regulated virulence in vivo, we performed dual RNA-seq to analyze transcriptomic changes in both C. elegans and P. aeruginosa. We found that C. elegans responded to P. aeruginosa {Delta}pvmSR infection by decreasing expression of lysosome and phagocytosis pathways. In P. aeruginosa {Delta}pvmSR, we observed decreased gene expression of several known virulence factors including the hydrogen cyanide synthase, hcnC, and heparinase, hepP. Additionally, we observed dysregulation in genes important for quorum sensing and biofilm formation. Collectively, our findings indicated that PvmSR contributed to virulence regulation and may serve as a potential anti-virulence target.

microbiology↗

bioRxiv2020

Metagenome-Assembled Genome Binning Methods Disproportionately Fail for Plasmids and Genomic Islands

Metagenomic methods are an important tool in the life sciences, as they enable simultaneous characterisation of all microbes in a community without time-consuming and bias-inducing culturing. Metagenome-assembled genome (MAG) binning methods have emerged as a promising approach to recover individual genomes from metagenomic data. However, MAG binning has not been well assessed for its ability to recover mobile genetic elements (MGEs), such as plasmids and genomic islands (GIs), that have very high clinical/agricultural/environmental importance. Certain antimicrobial resistance (AMR) genes and virulence factor (VF) genes are noted to be disproportionately associated with MGEs, making studying their transmission a public health priority. However, the variable copy number and sequence composition of MGEs relative to the majority of the host genome makes them potentially problematic for MAG binning methods. To systematically investigate this, we simulated a low-complexity metagenome comprising 30 GI-rich and plasmid-containing bacterial genomes. MAGs were then recovered using 12 current prediction pipelines and evaluated for recovery of MGE-associated AMR/VF genes. Here we show that while 82-94% of chromosomes could be correctly recovered and binned, only 38-44% of GIs were recovered and, even more notably, only 1-29% of plasmid sequences were found. Most strikingly, no plasmid-borne VF or AMR genes were recovered and within GIs, only between 0-45% of AMR or VF genes were identified. We conclude that short-read MAGs are largely ineffective for the analysis of mobile genes, including those of public-health importance like AMR and VF genes. We propose that microbiome researchers should instead primarily utilise unassembled short reads and/or long-read approaches to more accurately analyse metagenomic data

bioinformatics↗