Biology subjects

Lango, T.

Publications and source records attributed to Lango, T..

3 recordsLinked to original sources

bioRxiv2025

A549 tumorigenic and BEAS-2B non-tumorigenic cell line derived small extracellular vesicles show distinct proteomic, N-glycoproteomic and chondroitin/dermatan sulfate profiles

Extracellular vesicles (EVs) are critical mediators of intercellular communication and hold promise as biomarkers and therapeutic targets in cancer, but their molecular alterations remain poorly understood. Protein glycosylation is a frequent post-translational modification; however, most EV studies focus only on proteomics, while mapping glycosylation changes of proteins are still underrepresented. To address this shortcoming, we analyzed the proteomic, N-glycoproteomic, and chondroitin/dermatan sulfate (CS/DS) glycosaminoglycan (GAG) profiles of small EVs (sEVs) derived from A549 lung adenocarcinoma and BEAS-2B non-tumorigenic epithelial cell lines. Principal component analysis and hierarchical clustering revealed that all three profiles are highly dependent on the origin of sEV, highlighting fundamental differences not only at the proteomic but also at the N-glycopeptide and CS/DS levels. Protein expression differences were primarily associated with the upregulation of cell cycle regulation, DNA repair, metabolism, and protein synthesis, while immune-related processes were predominantly downregulated. Proteomics revealed differential expressions of 5 CS proteoglycans, anticipating that their CS profile may also change. N-glycoproteomics highlighted a shift from complex to hybrid N-glycans in cancer sEVs, alongside a significant decrease in fucosylation. Prominent glycoproteins characterized with multiple glycosylation sites included versican, galectin-3-binding protein and laminins. The total amount of CS/DS increased 3.4-fold in cancer sEVs, while the ratio of the two monosulfated disaccharides changed 2-fold, suggesting altered sulfation mechanisms. These findings highlight the potential of N-glycoproteomics and GAG profiling to enhance biomarker discovery and EV-based cancer diagnostics. Graphical abstract O_FIG O_LINKSMALLFIG WIDTH=200 HEIGHT=121 SRC="FIGDIR/small/643059v1_ufig1.gif" ALT="Figure 1"> View larger version (23K): org.highwire.dtl.DTLVardef@c2dfdeorg.highwire.dtl.DTLVardef@1f79452org.highwire.dtl.DTLVardef@12a9dbborg.highwire.dtl.DTLVardef@d992da_HPS_FORMAT_FIGEXP M_FIG C_FIG Proteomic, N-glycoproteomic and chondroitin/dermatan sulfate disaccharide profiles differ between A549 lung adenocarcinoma and BEAS-2B non-tumorigenic epithelial cell derived small extracellular vesicles.

cancer biology↗

bioRxiv2024

Design and Technical Feasibility Testing of a Medical Robot for Flexible Catheter Navigation Inside the Lung Airways

The integration of medical robots is revolutionizing clinical medicine, especially in procedures requiring precision in instrument manipulation and navigation within the body using medical imaging techniques like fluoroscopy, CT, and MRI. This is particularly challenging in peripheral lung lesion examinations, where guiding long, flexible instruments through the lung airways to the target exposes medical professionals and patients to harmful X-ray radiation. Several robotic approaches exist but there are still shortcomings in terms of their large footprint in the operating room and complex and costly mechanical structure. The goal of our research was to develop and test the early feasibility of RoboCath, an innovative robotic platform designed for the adaptable navigation of long, flexible catheter-like medical instruments through the lung airways. RoboCath seamlessly integrates with existing medical devices, such as bronchoscopes, facilitating access to lower lung regions with precision. Its compact design is ideal for crowded operating rooms, ensures sterilization compatibility, and supports a broad range of procedures, including those requiring intricate instrument manipulation and medical imaging for navigation. This technology significantly reduces the reliance on X-ray, thereby minimizing radiation exposure to both healthcare providers and patients. The RoboCath system represents a significant advancement in the field of medical robotics, offering a novel solution to the challenges of lung lesion biopsy and diagnosis, with potential applications in various open orifice procedures.

bioengineering↗

bioRxiv2020

Putative linear motifs mediate the trafficking to apical and basolateral membranes

Cell polarity refers to the asymmetric organisation of cellular components in various cells. Epithelial cells are the best known examples of polarized cells, featuring apical and basolateral membrane domains. Despite huge efforts, the exact rules governing the protein distribution in such domains are still elusive. In this study we examined linear motifs accumulating in these parts and based on the results we prepared Classical and Convolutional Neural Networks to classify human transmembrane proteins localizing into apical/basolateral membranes. Asymmetric expression of drug transporters results in vectorial drug transport, governing the pharmacokinetics of numerous substances, yet the data on how proteins are sorted in epithelial cells is very scattered. The provided dataset may offer help to experimentalists to characterize novel molecular targets to regulate transport processes more precisely.

bioinformatics↗