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LIU, Y.

Publications and source records attributed to LIU, Y..

11 recordsLinked to original sources

Alpha Spatial: Language-guided prediction of single-cell spatial gene expression from histology

Routine histology preserves tissue architecture but leaves its molecular organization largely unmeasured. Recovering this information requires learning across heterogeneous gene panels and constraining a biologically ill-posed morphology-to-expression mapping. Here we present Alpha Spatial (AS), a language-conditioned framework that predicts cell-level gene expression from histology while jointly learning from different tissues and panels. Matched biological context improved prediction over omitted context, whereas incompatible context reduced accuracy. Multi-tissue learning further improved breast-tissue prediction, and AS outperformed the evaluated methods while preserving molecular-state organization. In breast cancer, AS recovered POSTN-associated stromal programmes and HER2-associated molecular and spatial differences. Exploratory patient-level analyses identified a CXCL14-associated spatial state whose abundance was associated with non-response to trastuzumab-containing treatment and larger residual invasive tumours. These findings establish a framework for reusing heterogeneous molecular observations to investigate tissue organization and treatment-associated microenvironments in archived histology.

bioinformatics↗

Isolation, extraction, and physicochemical characterization of exosome-like nanovesicles from ginseng adventitious roots in tissue culture

This study aimed to establish a method for isolating exosome-like nanovesicles from ginseng adventitious roots and their culture supernatant, to investigate the effects of methyl jasmonate (MeJA) induction on vesicle yield and composition, and to provide experimental evidence for the scalable production of ginseng-derived exosome-like nanovesicles. Exosome-like nanovesicles were isolated from ginseng adventitious roots and culture supernatant using low-speed centrifugation combined with stirred-cell ultrafiltration. Physicochemical characterization was performed using transmission electron microscopy (TEM), nanoparticle tracking analysis (NTA), Zeta potential analysis, and Q-ToF LC-MS lipidomics. Ginseng adventitious roots were treated with 200 M MeJA to evaluate changes in vesicle particle concentration, total protein, total saponins, and miRNA expression before and after induction. Paraffin section fluorescence staining was used to observe the distribution of vesicle-related signals in adventitious root tissues. Exosome-like nanovesicles with typical bilayer membrane structures were isolated from both ginseng adventitious roots and culture supernatant.The particle size ranged from 77.333 to 171.733 nm, and the particle concentration ranged from 2.1E+8 to 7.6E+10 /mL. The vesicles contained lipid components including glycerides, phospholipids, and sphingolipids. Induction with 200 M MeJA increased vesicle particle concentration and total protein content, significantly upregulated the total saponin level in vesicles derived from adventitious roots, and altered the miRNA expression pattern of the vesicles. Tissue section fluorescence observation showed overlap between vacuolar markers and membrane vesicle dye signals. Low-speed centrifugation combined with ultrafiltration can effectively prepare ginseng-derived exosome-like nanovesicles. MeJA induction can regulate vesicle yield and active components, providing foundational data for the scalable process development of such vesicles.

plant biology↗

Metabolic Targeting of SREBP1 Reprograms the Obesity-Driven Ascites Immune Microenvironment and Enhances Chemotherapy Response in Obesity-Associated Ovarian Cancer

Obesity has an adverse effect on survival of women with ovarian cancer (OC), supporting a link between obesity, metastatic progression, and therapeutic response, but comprehensive mechanistic insight is lacking. In pre-clinical models of diet-induced obesity and OC, mice fed a high fat diet (HFD) exhibit substantially increased tumor burden relative to low fat diet (LFD) mice and respond poorly to standard-of-care (SOC) chemotherapy. Both HFD murine tumors and tumors from women with BMI>30 show increased expression and nuclear localization of sterol regulatory element binding protein 1 (SREBP1), a master regulator of lipogenesis and lipid transport. The objective of this study was to assess whether inhibition of SREBP1 processing could improve SOC chemotherapy response in obesity-associated OC. We evaluated the FDA-approved drug Nelfinavir (NFV), a pharmacologic inhibitor of site-2 protease that blocks SREBP1 processing, in combination with SOC (paclitaxel+carboplatin) chemotherapy in preclinical models of diet-induced obesity and OC. Our results demonstrate significantly improved therapeutic response, corresponding changes in intra-tumoral adipocytes, and reprogramming of the obesity-driven ascites immune microenvironment. These findings support a model in which NFV-mediated inhibition of SREBP1 processing disrupts obesity-associated metabolic pathways linked to immune suppression and therapeutic resistance, thereby enhancing response to SOC chemotherapy in obesity-associated OC. Collectively, these results highlight metabolic targeting as a strategy to improve therapeutic outcomes in obesity-associated ovarian cancer.

cancer biology↗

STR-PG: A Topology-decoupled Pangenome Framework for Scalable Short-read Genotyping of Short Tandem Repeats

Short tandem repeats (STRs) are a rich and highly polymorphic source of human genetic variation, but representing and genotyping them in pangenome graphs remains challenging. Explicitly encoding each STR allele as a separate graph path results in increasingly complex local structures as cohort diversity increases, leading to larger index sizes and requiring significant resources for graph reconstruction when new alleles are introduced. Here, we propose STR-PGa topologically decoupled genome-wide framework that separates stable locus representation from scalable STR allele content. STR-PG uses topologically fixed pointer nodes to represent each target locus, while allele sequences, repeat counts, motif annotations, and population frequency metadata are stored in an external registry. Short reads are mapped to STR loci via syncmer-based flanking anchors, and genotyping is performed within a locus-specific candidate space using allele-level alignment likelihood and Bayesian inference. Newly supported alleles can be integrated through registry-level updates without the need to rebuild the graph structure. Evaluations using simulated whole-genome sequencing data, 1000 Genomes Project (1kGP) samples, and r real whole-exome sequencing data from matched whole-blood-cell controls demonstrate that STR-PG maintains accurate genotyping results across various STR classes, reproduces expected population structures, and substantially reduces the computational cost of integrating additional alleles. STR-PG provides a compact and scalable framework for population-scale STR analysis using short-read sequencing.

bioinformatics↗

Microbial colonization establishes stratified radial niches that coordinate host epithelial and immune maturation in the colon

Microbial colonization is essential for intestinal maturation, yet how spatial organization of the microbiome shapes host tissue function remains unresolved. Here, we applied Stereo-seq V2 spatial platform to simultaneously profile the host transcriptome at single- cell resolution and microbial meta-transcriptome at 5 x 5 m resolution across the proximal, middle, and distal colon of germ-free (GF) mice and mice reconstituted by fecal microbiota transplantation (FMT), integrated with time-course fecal metagenomics. We observed that, four weeks after FMT, microbial colonization established a mature colonic architecture, increased goblet cell number and mucus layer thickness, diversified epithelial lineages, and expanded stem/transit-amplifying, myeloid, and T-cell populations. Metagenomic profiling showed succession from early colonizers to a metabolically mature, short-chain fatty acid (SCFA)-producing community that stabilized by four weeks. Distance-resolved spatial analysis resolved two reproducible strata of colonized microbiota along the radial host-lumen axis, separated at approximately 150 m. The epithelium-proximal stratum was enriched for mucus-associated taxa such as Bacteroides thetaiotaomicron, whereas the luminal stratum harbored fiber-associated taxa such as Ruminococcus champanellensis. This radial organization was underpinned by co-occurrence networks of spatial co-localization and co-exclusion. Finally, we identified a butyrate-producing guild that preferentially colonized the epithelium-proximal stratum, localized closer to epithelial and stromal cells, and showed active butyrate- responsive transcriptional activity. Colonization therefore establishes a spatially integrated host-microbiome interface with quantifiable, stratified microbial niches in which location, and not composition alone, coordinates epithelial and immune maturation.

microbiology↗

MXRA7 Alleviates Epididymitis from Exercise-Induced Fatigue by Inhibiting Pyroptosis

AimsTo explore exercise-induced fatigue (EIF)s effects on the male reproductive system and MXRA7s regulatory role herein. MethodsWe recruited EIF volunteers for semen/serum tests, established a mouse EIF model via weight-loaded swimming to assess epididymal segmental injury, and constructed pyroptosis models of PC-1/DC-2 cells. Public database transcriptomic analysis identified MXRA7 expression and enriched pathways in epididymitis; MXRA7s function was verified via its knockdown/overexpression in DC-2 cells. PKC-MXRA7 association was explored by phosphorylation assays and CO-IP, and sperm incubation experiments evaluated MXRA7s effect on sperm function. ResultsEIF impaired human sperm motility, reduced mouse sperm quality and induced epididymitis with segment-specific pyroptosis. MXRA7 expression differed in PC-1/DC-2 cells and correlated with pyroptosis; it was phosphorylated by PKC, inhibited the NF-{kappa}B pathway to alleviate inflammation, and mitigated pyroptosis-induced sperm motility damage. ConclusionEIF induces epididymal epithelial pyroptosis and epididymitis, and MXRA7 exerts a protective effect mainly in caudal epididymal cells by alleviating pyroptosis, thus reducing sperm quality damage.

molecular biology↗

Architecture and mechanism of a dual-enzyme retron system in prokaryotic immunity

Retrons are bacterial genetic retroelements encoding a reverse transcriptase (RT) and a non-coding RNA (ncRNA)-multi-copy single-stranded DNA (msDNA) hybrid. Diverse effector proteins or domains are found to associate with retrons, typically forming tripartite toxin-antitoxin systems involved in anti-phage defense. Although retrons have attracted growing interest in genome editing technologies, the mechanisms underlying most retron-mediated immune systems remain poorly understood. Here, we characterized a distinct quaternary retron system, Ec78, harboring a dual-enzymatic effector complex, in which the PtuA ATPase and PtuB nuclease act in concert to mediate phage clearance. The cryo-EM structure of the Ec78 complex adopts a flower-basket-like architecture, with two Ec78 retrons engaging the PtuAB effector complexes through a unique msDNA-insertion assembly mechanism. Interestingly, a sensing loop on the RT protein tightly monitors the length of the msDNA, which is likely responsible for phage detection and the subsequent release of the toxic effector complex. We further determined the cryo-EM structure of the retron-unbound effector complex, revealing an arginine-lysine finger loop on the PtuB nuclease that undergoes an ordered-to-disordered transition for enzymatic activation. Together, our work not only delineates the molecular basis underlying the Ec78 system in antiviral defense but also highlights the mechanistic diversity of retron systems in prokaryotic immunity.

biochemistry↗

Angiopoietin-like 4 shapes the intrahepatic T-cell landscape via eIF2α signalingduring steatohepatitis in diet-induced NAFLD.

Adaptive T-cell immune response is essential in conferring protective immunity, a process requiring tight cellular homeostasis regulation. Pathological intrahepatic T-cell landscape has a role in NAFLD propagation; however, its activation remains unknown. To address this gap, we extensively characterized a novel diet-induced NAFLD murine model (LIDPAD) featuring key phenotypic and genetic attributes reflective of human NAFLD. Comparative transcriptomic-guided staging of human and murine NASH reinforced the robustness of LIDPAD in recapitulating critical transitory stages of human NAFLD. We found that angiopoietin-like 4 (Angptl4) shapes activation of the intrahepatic T-cell landscape through the modulation of eIF2 signaling during fibrosis. Single-immune cell analysis and hepatic transcriptomics during fibrosis, and kinase inhibitor screening confirmed that Angptl4 orchestrates the hyperactivation of intrahepatic adaptive immunity via eIF2 signaling. Consistently, immunoblocking of cAngplt4 reduces T-cell overactivation, delaying disease aggravation. Taken together, Angptl4 is a crucial determinant in shaping intrahepatic adaptive immunity during fibrosis in NAFLD.

immunology↗

Genetic engineering of Acidithiobacillus ferridurans with CRISPR-Cas9/dCas9 systems

Genus Acidithiobacillus includes a group of Gram-negative Fe/S-oxidizing acidophilic chemolithotrophic bacteria that are extensively studied and used for biomining processes. Synthetic biology approaches are key means to study and improve their biomining performance. However, efficient genetic manipulations in Acidithiobacillus are still major bottlenecks. In this study, we report a simple and efficient pAFi system (CRISPR-dCas9) and a scarless pAF system (CRISPR-Cas9) for genetic manipulations in A. ferridurans JAGS. The pAFi system harboring both dCas9 and sgRNA was constructed based on pBBR1MCS-2 to knockdown HdrA and TusA genes, separately, of which the transcription levels were significantly downregulated by 48% and 93%, separately. The pAF system carrying pCas9-sgRNA-homology arms was constructed based on pJRD215 to delete HdrB3 gene and overexpress Rus gene. Our results demonstrated that the pAF system is a fast and efficient genome editing method with an average rate of 15-20% per transconjugant in one recombination event, compared to 10-3 and then 10-2 in two recombination events by traditional markerless engineering strategy. Moreover, with these two systems, we successfully regulated iron and sulfur metabolisms in A. ferridurans JAGS: the deletion of HdrB3 reduced 48% of sulfate production, and substitution overexpression of Rus promoter showed 8.82-fold of mRNA level and enhanced iron oxidation rate. With these high-efficient genetic tools for A. ferridurans, we will be able to study gene functions and create useful recombinants for biomining applications. Moreover, these systems could be extended to other Acidithiobacillus strains and promote the development of synthetic biology-assisted biomining. HighlightsO_LITwo shuttle vectors were constructed for Acidithiobacillus ferridurans C_LIO_LIAll-in-one pAFi (CRISPR-dCas9) and pAF (CRISPR-Cas9) systems were built up for gene knockdown and genome editing, separately C_LIO_LIThe transcription levels of HdrA and TusA were reduced 48% and 93% using pAFi system and thus suppressed sulfur oxidation C_LIO_LIHdrB3 deletion and Rus overexpression were achieved using pAF system and showed significant effects on sulfur and iron oxidation respectively C_LIO_LIOur pAF system facilitated genome editing in Acidithiobacillus ferridurans with high efficiency (15-20%) in less than 4 weeks C_LI

bioengineering↗

Spatiotemporal transcriptome at single-cell resolution reveals key radial glial cell population in axolotl telencephalon development and regeneration

Brain regeneration requires a precise coordination of complex responses in a time- and region-specific manner. Identifying key cell types and molecules that direct brain regeneration would provide potential targets for the advance of regenerative medicine. However, progress in the field has been hampered largely due to limited regeneration capacity of the mammalian brain and understanding of the regeneration process at both cellular and molecular level. Here, using axolotl brain with extrodinary regeneration ability upon injury, and the SpaTial Enhanced REsolution Omics-sequencing (Stereo-seq), we reconstructed the first architecture of axolotl telencephalon with gene expression profiling at single-cell resolution, and fine cell dynamics maps throughout development and regeneration. Intriguingly, we discovered a marked heterogeneity of radial glial cell (RGC) types with distinct behaviors. Of note, one subtype of RGCs is activated since early regeneration stages and proliferates while other RGCs remain dormant. Such RGC subtype appears to be the major cell population involved in early wound healing response and gradually covers the injured area before presumably transformed into the lost neurons. Altogether, our work systematically decoded the complex cellular and molecular dynamics of axolotl telencephalon in development and regeneration, laying the foundation for studying the regulatory mechanism of brain regeneration in the future.

developmental biology↗

Functional analysis of the teosinte branched 1 genes in the tetraploid switchgrass (Panicum virgatum L.) by CRISPR/Cas9-directed mutagenesis

Tillering is an important biomass yield component trait in switchgrass (Panicum virgatum L.). Teosinte branched 1 (tb1)/Branched 1 (BRC1) gene is a known regulator for tillering/branching in several plant species; however, its role on tillering in switchgrass remains unknown. Here, we report physiological and molecular characterization of mutants created by CRISPR/Cas9. We successfully obtained non-chimeric Pvtb1a and Pvtb1b mutants from chimeric T0 mutants using nodal culture. The biallelic Pvtb1a-Pvtb1b mutant plants produced significantly more tillers and higher fresh weight biomass than the wild-type plants. The increased tiller production in the mutant plants resulted primarily from hastened outgrowth of lower axillary buds. Increased tillers were also observed in transgene-free T1 monoallelic mutants for either Pvtb1a-Pvtb1b or Pvtb1b gene alone, suggesting Pvtb1 genes act in a dosage-dependent manner. Transcriptome analysis showed 831 genes were differentially expressed in the Pvtb1a-Pvtb1b double knockdown mutant. Gene Ontology analysis revealed downregulation of Pvtb1 genes affected multiple biological processes, including transcription, flower development, cell differentiation, and stress/defense responses in edited plants. This study demonstrates that Pvtb1 genes play a pivotal role in tiller production as a negative regulator in switchgrass and provides opportunities for further research aiming to elucidate the molecular pathway regulating tillering in switchgrass. HighlightSolid non-chimeric mutants were successfully isolated from CRISPR/Cas9-induced chimeric mutants using nodal culture. Teosinte branched 1 (tb1) genes are involved in various pathways to regulate tillering in switchgrass.

plant biology↗