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Kundanati, L.

Publications and source records attributed to Kundanati, L..

8 recordsLinked to original sources

Upcycling banana peduncle fibers into mycelium-based composites for sustainable packaging and thermal insulation

The growing concerns due to plastic pollution in India have intensified the search for sustainable materials. Mycelium-based composites (MBCs) have emerged as bio-based alternatives for packaging and thermal insulation applications. India, the worlds largest producer of bananas, generates significant quantities of banana biomass (~200 tons per hectare per year), much of which remains underutilized. The banana peduncle, the stalk that supports the fruit bunch, is one such underutilized biomass. In this study, banana peduncle fibers were used as the main substrate with Pleurotus ostreatus for the fabrication of MBCs. Banana peduncle fibers were mixed with wood shavings (10-50 wt%) to enhance the dimensional stability and structural integrity of the composites. The properties of developed MBCs such as density, shrinkage, moisture absorption, water absorption, morphology, compressive properties, and thermal conductivity were studied. The 90% banana peduncle fibers-10% wood shavings formulation showed the highest radial mycelial growth rate (7 mm/day). MBCs consisting of 100% banana peduncle fibers had volumetric shrinkage of 36%, while the incorporation of 30-50% wood shavings reduced shrinkage by approximately 17%. Among the formulations, MBCs containing 30% wood shavings had the highest compressive strength (4.82 MPa) and compressive modulus (1.78 MPa), whereas MBCs containing 50% wood shavings had the highest recovery (59.6%). In contrast, MBCs fabricated using 100% banana peduncle fibers had the lowest thermal conductivity (0.04 W/m. K). These results demonstrate that banana peduncle fibers are a promising lignocellulosic substrate for the development of MBCs for sustainable packaging and thermal insulation.

bioengineering↗

Small Intestinal Submucosa (SIS)-dECM Bioink with Extrusion-Induced Collagen Organization for Tympanic Membrane Tissue Engineering

Tympanic membrane perforations remain a common clinical problem, and although surgical intervention through tympanoplasty achieves high success rates, it is associated with donor-site morbidity, surgical complexity and limited restoration of the native radial and circumferential collagen architecture. In this study, 3D extrusion printing was utilized to create an active scaffold and attempt to promote collagen organization through shear-mediated structural alignment. An alginate-carboxymethyl cellulose (CMC) hydrogel with bovine SIS-dECM was prepared and investigated for its suitability as a bioink alternative to tympanoplasty grafts. The physiochemical, rheological and printability characteristics of the hydrogel were assessed. Successful decellularization was confirmed by histological analysis. The incorporation of the SIS-dECM into the hydrogel led to increased swelling, lower apparent viscosity, yield stress and flow stress while maintaining favourable printability and filament stability. Polarized optical microscopy was also used to study the influence of printing speed on the alignment of collagen to mimic the native tympanic membrane radial collagen architecture. Compared with the cast controls, the printed samples presented stronger birefringence signals. Biological evaluation demonstrated that the 15% dECM hydrogel exhibited the highest live cell area percentage and live/dead ratio after 48 h. In addition, the chick chorioallantoic membrane assay demonstrated that the dECM-containing hydrogels improved vascular density. The findings establish a printable, biologically active dECM bioink capable of generating bulk collagen organization through extrusion printing as a platform for tympanic membrane regeneration.

bioengineering↗

Growth characteristics of Myco-Composites Produced Using Locally Isolated Ganoderma Species and Lignocellulosic Waste

This study reports the development of myco-composites from locally isolated Ganoderma species and regional lignocellulosic wastes such as paper, cardboard, sawdust, and wood shavings. Eight substrate formulations were evaluated for fungal colonization and composite quality. Visual inspection revealed that fine, fibrous substrates like paper and cardboard achieved the most uniform mycelial colonization and highest densities (0.45 and 0.41 g/cm3, respectively), while coarse, porous substrates such as wood shavings and mixed substrates (M1 and M2) produced lighter, less dense composites (0.27 g/cm3). Volumetric shrinkage ranged from 7.1% to 26.7%, with lower values in cardboard and higher in wood shavings. FTIR spectroscopy confirmed the presence of characteristic fungal and lignocellulosic functional groups in mixed composites. These findings demonstrate that composite density, dimensional stability, and chemical structure are strongly influenced by substrate type and proportion, highlighting the potential for tailored myco-composite materials using local waste resources. Graphical Abstract O_FIG O_LINKSMALLFIG WIDTH=200 HEIGHT=65 SRC="FIGDIR/small/690336v1_ufig1.gif" ALT="Figure 1"> View larger version (23K): org.highwire.dtl.DTLVardef@d54a45org.highwire.dtl.DTLVardef@ff08daorg.highwire.dtl.DTLVardef@19e29f4org.highwire.dtl.DTLVardef@d46577_HPS_FORMAT_FIGEXP M_FIG C_FIG

bioengineering↗

Development and Characterization of 3D Printable Caprine derived dECM/DAS Crosslinked Hydrogels for Tissue Engineering Applications

The current hydrogels and crosslinkers used in tissue engineering often suffer from limited bioactivity and insufficient mechanical strength, which hinders effective tissue regeneration. To overcome these limitations, this study aims to develop a tunable hydrogel combining caprine-derived decellularized Extracellular Matrix (dECM) and dialdehyde starch (DAS) for potential use in tissue engineering. The dECM bioink was biohybridized with varying concentration of DAS crosslinker to create dECM/DAS hydrogels and its physicochemical and biological properties were characterized. FTIR analysis confirmed DASs successful formation through a strong carbonyl peak at 1732 cm-1. The rheological characterization indicates the shear-thinning behavior for dECM/DAS. The storage modulus was higher than that of the loss modulus in all the concentrations of DAS crosslinked hydrogel compared to dECM hydrogel. The gelation kinetics show that dECM and DAS crosslinked hydrogel begin to gel at temperatures above 25{degrees}C, which is suitable for developing implantable hydrogel. The hemocompatiblity study shows that the dECM and dECM/DAS hydrogels are non-hemolytic at less than 2%. Overall, this naturally derived dECM/DAS hydrogel demonstrates the potential to be utilized for tissue engineering applications.

bioengineering↗

Mycelium Biocomposites from Agricultural and Paper waste: Sustainable alternative to plastic foam based secondary packaging

Plastic foams, which were once lauded as a breakthrough invention in packaging and insulation industries, have gradually evolved into a global problem and are more concerning in developing nations because of ineffective waste management systems. The convenience and pervasiveness of these come at a high cost with adverse effects on human health and our planets ecosystems as they are not biodegradable. It is pivotal that an alternative and sustainable solution is found to address this problem. Mycelium-based biocomposites, made up of root-like networks called hyphae are sought out as a potential substitute for plastic-based foams as they are biodegradable. They employ a waste-to-value strategy by using agricultural wastes as a growing media for the mycelium. The study primarily focuses on the comparative analysis of mycelium-based biocomposites with existing foam materials such as Expanded PolyStyrene (EPS) and evaluates their efficacy as a biodegradable packaging material. Thus, we plan to identify the efficacious strain of mycelium from Pleurotus ostreatus or Ganoderma lucidum and also explore various substrate materials such as cocopith, cardboard, paper, and sawdust. Our preliminary compression results show that the biocomposites can be engineered to have Youngs modulus ranging from 1-3 MPa for both strains. These results show that mycelium-based biocomposites can be a promising alternative to EPS /Styrofoam and move towards a sustainable solution that can reduce the dependence on non-biodegradable foam-based materials in developing nations like India.

bioengineering↗

Mechanics of snake biting: Experiments and modelling

Among all the vertebrates, snakes possess the most sophisticated venom delivering system using their fangs. Fangs of many animals are well adapted to the mechanical loads experienced during the functions such as breaking the diet and puncturing the skin of the prey. Thus, investigation and modelling of puncturing mechanics of snakes is of importance to understand the form-function relationship of the fangs and tissue-fang interactions in detail. We have thus chosen fangs of two snake species i.e. viper (Bitis arietans) and burrowing snake (Atractaspis aterrima), with different shape and size and performed insertion experiments using tissue phantoms. Our results showed that both the species have similar mechanical properties but there was a difference in the insertion forces owing to the difference in shape of the fang. Also, our modelling of the fang-tissue interactions predicted some parameters close to the experimental values. Thus, our study can help in the development of bioinspired needles that can potentially have reduced insertion forces and less damage to the tissue.

bioengineering↗

Mechanics of wood boring by beetle mandibles

Wood boring is a feature of several insect species and is a major cause of severe and irreparable damage to trees. Adult females typically deposit their eggs on the stem surface under bark scales. The emerging hatchlings live within hard wood during their larval phase, avoiding possible predation, whilst continually boring and tunneling through wood until pupation. A study of wood boring by insects offers unique insights into the bioengineering principles that drive evolutionary adaptations. We show that larval mandibles of the coffee wood stem borer beetle (Xylotrechus quadripes: Cerambycidae) have a highly sharp cusp edge to initiate fractures in Arabica wood and a suitable shape to generate small wood chips that are suitable for digestion. Cuticle hardness at the tip is significantly enhanced through zinc-enrichment. Finite element model of the mandible, based on the differential material properties at the tip, intermediate and base regions of the mandible, showed highest stresses in the tip region; these decreased to significantly lower values at the start of the hollow section. A hollow architecture significantly reduces bending stresses at mandibular base without compromising the structural integrity. A scaling model based on a fracture mechanics framework shows the importance of the mandible shape in generating optimal chip sizes. These findings contain general principles in tool design and put in focus interactions of insects and their woody hosts.

bioengineering↗

Prey capturing and feeding apparatus of dragonfly nymph

Aquatic predatory insects, like the nymphs of a dragonfly, use rapid movements to catch their prey. Dragonfly nymphs are voracious predators that feed on smaller aquatic organisms. In this study, we examine dragonfly nymph (Libellulidae: Insecta: Odonata) mouthparts that are used in prey capturing and feeding. In particular, we characterise the morphology of the labium and mechanical properties of the mandibles of the nymph. Additionally, we record and analyse the preying mechanism using high-speed photography. The morphological details suggest that the prey capturing mechanism is a complex grasping mechanism with additional sensory organs that might aid in sensing the surroundings. The times taken for the extension and retraction of labial organ during prey capture was 187{+/-}54 ms. The Youngs modulus and hardness of the mandibles samples were 9.1{+/-}1.9 GPa and 0.85{+/-}0.13 GPa. Gradation in the mechanical properties was also observed in the mandible tip regions with increased properties at the tip end. The overall mechanism with its sensory capabilities provides a unique design to develop bioinspired underwater deployable mechanisms.

bioengineering↗