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Kumar, K. A.

Publications and source records attributed to Kumar, K. A..

2 recordsLinked to original sources

Breeding and identification of promising Mauritius x Kew pineapple hybrids with high heterosis for fruit and plant traits

Leading cultivars of pineapple Mauritius and Kew were hybridized and 25 hybrids were evaluated under open field conditions, using randomized block design with two replications. Performance of the female parent cum check variety Mauritius, male parent Kew and check variety in Kerala state, India, Amritha, were also evaluated and compared. Based on the performance, heterobeltiosis, average heterosis and standard heterosis over two check varieties, in each hybrid, for 10 plant growth traits and 24 fruit traits, were calculated. For fruit weight, hybrid H35 had the highest heterobeltiosis and standard heterosis over Amritha whereas H62 had highest standard heterosis over Mauritius and average heterosis. For pulp weight, hybrid H17 had the highest values for all heterosis parameters. For TSS, hybrid H62 had the highest heterobeltiosis and other parameters were highest in H43. For days to attain physiological maturity, crown weight, peel weight and acidity, H27, H30, H77 and H43, respectively were lowest in all heterosis parameters. Based on the selection criterion [{sum}average heterosis (fruit weight, TSS, pulp weight) - {sum}average heterosis (crown weight, peel weight, eye profile, eye relative surface, time taken for physiological maturity, acidity)] developed using the average heterosis values for desirable and undesirable fruit traits, six hybrids H66, H17, H59, H43, H70 and H35 were identified for further evaluation. The identified hybrids also satisfied the requirements in fruit weight ([≥]1.0 kg), pulp weight ([≥]750.58 g), TSS ([≥]14.49 {circ}Brix), days to attain physiological maturity ([≤]185.70 days), crown weight ([≤]305.50 g), peel weight ([≤]159.27 g) and acidity ([≤]1.05).

plant biology↗

Crystal structure and activity profiling of deubiquitinating inhibitors-bound to SARS-CoV-2 papain like protease revealed new allosteric sites for antiviral therapies

SARS-CoV-2 papain-like protease (PLpro) is a key antiviral target as it plays a dual role in viral replication and in modulation of innate immune responses by deubiquitinating or deISGylating host proteins. Thus, therapeutic targeting of PLpro serves as a two-pronged approach to abate SARS-CoV-2. Interestingly, PLpro shares structural and functional similarities with the cellular deubiquitinating enzymes (DUBs) and in this study this fact has been exploited to identify DUBs inhibitors that target the Ubiquitin/ISG15 binding site and the known catalytic substrate binding pocket of PLpro. Among these identified compounds, flupenthixol, lithocholic acid, teneligliptin, and linagliptin markedly inhibited the proteolytic activity of purified PLpro and demonstrated potent antiviral efficacies against SARS-CoV-2 infection in a dose dependent manner. Treatment with lithocholic acid and linagliptin suppressed the expression levels of inflammatory mediators, thereby, restoring immune responses. Crystal structures of SARS-CoV-2 PLpro in complex with linagliptin and with lithocholic acid determined in this study, revealed insights into the inhibition mechanism with unique interactions within the Ubiquitin/ISG15 binding site (S2 site; Phe69, His73, Asn128, His175) and the substrate binding cleft. Additionally, oral and intraperitoneal treatments with linagliptin increased survival, reduced lung viral load, and ameliorated histopathological damage in mouse-adapted model of SARS-CoV-2 infection. The study for the first time demonstrates a two-pronged strategy using DUB inhibitors that target the proteolytic activity of PLpro and simultaneously reinstates the hosts immune response against SARS-CoV-2.

molecular biology↗