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Krzyzanowska, D. M.

Publications and source records attributed to Krzyzanowska, D. M..

6 recordsLinked to original sources

Beyond kin killing: Dickeya-derived phage-tail-like bacteriocin P2D1 targets phylogenetically distant Pseudomonas spp.

Tailocins, phage-tail-derived bacteriocins, are increasingly recognized as potent mediators of microbial antagonism, yet their ecological scope beyond kin-targeting remains poorly understood. Here, we investigated whether P2D1, a tailocin produced by the plant pathogen Dickeya dadantii 3937, can act against environmental bacteria phylogenetically distant from Dickeya spp. Screening 480 soil and rhizosphere isolates from three distinct plant-associated habitats in Poland, we identified nine Pseudomonas spp. strains susceptible to tailocin P2D1. Whole-genome sequencing and phenotype profiling revealed that these isolates spanned multiple clades, including taxa related to P. germanica, P. tensinigenes, and P. parakoreensis. The D. dadantii mutant lacking genes encoding tailocin sheath and tube proteins lost antagonistic activity against Pseudomonas isolates, confirming that tailocins alone mediate the observed killing. Plant tissue assays revealed that six of the P2D1-susceptible strains were non-pathogenic and could mitigate D. dadantii-induced soft rot on potato. In contrast, three isolates related to P. tensinigenes were able to cause rot on their own under permissive conditions. Together, these findings demonstrate that P2D1 tailocin extends its activity to ecologically co-occurring but taxonomically distant Pseudomonas, suggesting that conserved receptors underline cross-genus targeting. More broadly, our results add to the limited evidence for tailocin activity beyond kin killing and therefore challenge the prevailing paradigm of kin-restricted tailocin specificity. They further suggest that tailocins may influence microbial community assembly across taxonomic boundaries, while their in vivo roles remain understudied.

microbiology↗

Production of P2D1 tailocins by Dickeya dadantii 3937: a temporal relationship between the stressor onset, gene expression, and the concentration of active particles

Tailocins are bacteriocins resembling bacteriophage tails. Previously, we reported the production of P2D1 tailocins in the plant pathogen Dickeya dadantii 3937, the synthesis of which was upregulated upon treatment with mitomycin C. In the current study, we characterized the temporal relationship between the inducer onset, activation of expression of tailocin-related genes, the concentration of tailocin particles, and the titer of viable producer cells. Using a newly established RT-qPCR assay, we found that the expression of P2D1 structural genes peaks two hours after the addition of mitomycin C. Simultaneous measurement of the titer of tailocins showed that the maximum concentration of these particles in the culture supernatant was reached 6 hours post induction and remained stable till the termination of the experiment (18 hours). Progressing accumulation of tailocins between the second and the sixth-hour post mitomycin C treatment correlated with a sharp drop in the count of viable cells (ca. five orders of magnitude). This effect was related to the mechanism by which tailocins are released from the cells, which involves cell lysis. Our study is the first detailed analysis of the timing of events during P2D1 tailocin synthesis in Dickeya spp.

microbiology↗

Tailocin-mediated interactions in Soft Rot Pectobacteriaceae

Bacteria carry phage-derived elements within their genomes, some of which can produce phage-like particles (tailocins) used as weapons to kill kin strains in response to environmental conditions. This study investigates the production and activity of tailocins by plant pathogenic bacteria: Pectobacterium, Dickeya, and Musicola genera, which compete for niche, providing an attractive model to study the ecological role of tailocins. Microscopy revealed that most analyzed strains (88%) produced tailocins. Tailocin-mediated killing interactions were assessed across 351 strain pairs, showing that Dickeya spp. had a higher likelihood of killing neighbors (57.1%) than Pectobacterium spp. (21.6%). Additionally, Dickeya spp. strains exhibited broader phylogenetic killing, targeting both Pectobacterium spp. and Musicola sp., while Pectobacterium spp. tailocins were genus-specific. Mutual killing was observed in 33.9% of interactions, predominantly within Dickeya spp. Although tailocins were morphologically indistinguishable between producers, genomic analyses identified conserved clusters having distinct differences between Pectobacterium spp. and Dickeya spp tailocins. This suggests different origins of these particles. Induction experiments demonstrated that tailocin production was boosted by hydrogen peroxide, supporting the role of these particles in bacteria-bacteria competition during infection. Tailocins were detectable in infected potato tissue but not in river water, highlighting the particular ecological relevance of tailocins in plant environments.

microbiology↗

Soft rot pathogen Dickeya dadantii 3937 produces tailocins resembling the tails of Enterobacteria bacteriophage P2

Tailocins are nanomolecular machines with bactericidal activity. They are produced by bacteria to contribute to fitness in mixed communities, and hence, they play a critical role in their ecology in a variety of habitats. Here, we characterized the new tailocin produced by Dickeya dadantii strain 3937, a well-characterized member of plant pathogenic Soft Rot Pectobacteriaceae (SRP). Tailocins induced in D. dadantii were ca. 166 nm long tubes surrounded by contractive sheaths with baseplates having tail fibers at one end. A 22-kb genomic cluster involved in their synthesis and having high homology to the cluster coding for the tail of the Enterobacteriophage P2 was identified. The D. dadantii tailocins, termed dickeyocin P2D1 (phage P2-like dickeyocin 1), were resistant to inactivation by pH (3.5 - 12), temperature (4 - 50 {degrees}C), and elevated osmolarity (NaCl concentration: 0.01 - 1 M). P2D1 could kill a variety of different Dickeya spp. but not any strain of Pectobacterium spp. tested and were not toxic to Caenorhabditis elegans. TeaserTailocins are nanomolecular entities similar to syringes that are produced by various bacteria to fight other microorganisms present in the same environment.

microbiology↗

Shared and host-specific transcriptomic response of Pseudomonas donghuensis P482 to the exudates of tomato (Dicot) and maize (Monocot) shed light on the host-adaptive traits in the promiscuous root colonizing bacteria

Plants of different genotypes and physiological states recruit different populations of root microbiota. The selection is driven by the immune response of the plant and the composition of root exudates. Some bacteria, including Pseudomonas spp., are promiscuous root colonizers. It is yet unclear what particular changes in lifestyle enable them to thrive in the company of different plant hosts. In this study, we used RNAseq to identify genes of the differential (host-specific) and shared (host-independent) transcriptomic responses of a biocontrol strain Pseudomonas donghuensis P482 to the root exudates of two phylogenetically distinct plant species, tomato (Dicot) and maize (Monocot), both of which can be colonized by the bacterium. The host-independent response of P482 to exudates involved upregulated expression of arsenic resistance genes and bacterioferritin synthesis. Contrary, we observed downregulation of pathways related to sulfur assimilation, sensing of ferric citrate and/or other iron carriers, the acquisition of heme, the assembly of the type VI secretion system, and amino acid transport. Pathways upregulated in P482 specifically by tomato exudates included nitric oxide detoxification, repair of iron-sulfur clusters, respiration through the cyanide-insensitive cytochrome bd, and catabolism of amino acids and/or fatty acids. The maize-specific response included upregulation of genes associated with motility, the activity of MexE and two other RND efflux pumps, and copper tolerance. To provide more context to the study, we determined the chemical composition of exudates by GC-MS, NMR, and LC-SRM. Our results bring new insight into the host-driven metabolic adaptations of promiscuous root colonizing bacteria. Significance statementUnderstanding factors determining the composition and the activity of plant-associated microbiota is crucial to harnessing their potential to benefit plant health. Traits that enable microorganisms to colonize plants have long been the subject of study, with many important factors identified for particular host-microbe systems. However, studies involving more than a single plant host are rare. This results in many unanswered questions on the host-specific and universal aspects of metabolism that enable more promiscuous root colonizers to interact with different plant hosts. The presented study begins to fill this knowledge gap by providing data on the metabolic pathways involved in the differential and shared response of Pseudomonas donghuensis strain P482 to the exudates of phylogenetically distant plant species: tomato and maize.

microbiology↗

High-quality complete genome resource of tomato rhizosphere strain Pseudomonas donghuensis P482, a representative of a species with biocontrol activity against plant pathogens

Strain P482 was isolated from a tomato rhizosphere and classified as Pseudomonas donghuensis. The P. donghuensis species was first established in 2015 and currently comprises only four strains: P482, HYST, SVBP6, and 22G5. P. donghuensis strains antagonize plant pathogens, including bacteria, fungi and oomycetes, and therefore are of high interest regarding their biological control potential to combat plant diseases. The antimicrobial activity of P. donghuensis P482 is based on the production of iron scavenging compound 7-hydroxytropolone, antifungal volatile organic compounds, and yet unidentified secondary metabolite(s). Here, we report a complete genome resource for P. donghuensis strain P482. The genome consists of a single chromosome (5 656 185 bp) with 5258 ORFs (5158 protein-coding genes, 74 tRNAs, 22 rRNAs, 3 ncRNAs and 1 tmRNA) and no plasmid. We believe that the information of the first high-quality, complete genome of P. donghuensis will provide resources for analyses targeting the biological control potential of this species and understanding the traits essential for plant-microbe interaction.

genomics↗