Biology subjects

Kryukov, V.

Publications and source records attributed to Kryukov, V..

2 recordsLinked to original sources

bioRxiv2023

The metagenomic analysis of viral diversity in Colorado potato beetle public NGS data

The Colorado potato beetle (CPB) is one of the most serious insect pests with high ecological plasticity and ability to rapidly develop resistance to insecticides. The use of biological insecticides based on viruses is a promising approach to control insect pests, but the information on viruses, which infect leaf feeding beetles, is scarce. We performed the metagenomic analysis of 297 CPB genomic and transcriptomic samples from public NBCI SRA database. The reads that were not aligned to the reference genome were assembled with metaSPAdes and 13314 selected contigs were analyzed with BLAST tools. The contigs and non-aligned reads were also analyzed with Kraken2 software. 3137 virus-positive contigs were attributed to different viruses belonging to 6 types, 17 orders and 32 families, matching over 97 viral species. The annotated sequences can be divided into several groups: homologous to genetic sequences of insect viruses (Adintoviridae, Ascoviridae, Baculoviridae, Dicistroviridae, Chuviridae, Hytrosaviridae, Iflaviridae, Iridoviridae, Nimaviridae, Nudiviridae, Phasmaviridae, Picornaviridae, Polydnaviriformidae, Xinmoviridae etc.), plant viruses (Betaflexiviridae, Bromoviridae, Kitaviridae, Potyviridae), and endogenous retroviral elements (Retroviridae, Metaviridae). Also, the full-length genomes and near-full length genome sequences of several viruses were assembled. We have also found the sequences belonging to Bracoviriform viruses and for the first time experimentally validated the presence of bracoviral genetic fragments in CPB genome. Our work is the first attempt to discover the viral genetic material in CPB samples and we hope that further studies will help to identify new viruses to extend the arsenal of biopesticides against CPB. The analytical pipeline and additional materials are available at https://github.com/starchevskaya-maria17/uncoVir

bioinformatics↗

bioRxiv2022

Biopriming of broad bean seeds with entomopathogenic fungus Metarhizium robertsii does not affect invertebrate communities of the agroecosystem

Biopriming, or treatment of seeds with beneficial microorganisms such as beneficial fungi, can be a promising strategy in agricultural cultivation. However, the effects of such treatment on non-target organisms living in the soil and on plants have not been sufficiently studied, and it is not known very well whether such treatment would alter invertebrate communities (e.g., harm them). Here, we addressed the effect of treating broad bean seeds (Vicia faba L.) with the conidia of entomopathogenic ascomycete Metarhizium robertsii on the diversity and abundance of invertebrate communities in the agroecosystem in the south part of West Siberia in 2019 and 2020. We have analyzed the effect both on the general invertebrate communities as well as on the main pests of beans. In the case of bean pests, we assessed the rate of plant infestation by aphids (Hemiptera: Aphididae) and the degree of leaf damage by leafminer flies Liriomyza bryoniae (Diptera: Agromyzidae). In most cases, the treatment did not lead to significant changes in the total abundance of the soil invertebrates and herbivores or the abundance of predominant taxa (Coleoptera: Carabidae, Staphylinidae, Elateridae, Scarabaeidae, Curculionidae; Hemiptera: Miridae, Cicadellidae, Aphididae; larvae of Diptera). A positive effect of treatment on population density of the soil mesofauna was noted for Diptera larvae in June 2019. Regarding aphids and leafminer flies, no significant effect was observed in terms of the proportion of plants with aphids and the density of aphid colonies on individual plants throughout the season, and no significant influence was found on the proportion of plant leaves damaged by leafminer fly Liriomyza bryoniae larvae. In summary, in Western Siberia, the treatment of broad bean seeds with M. robertsii did not significantly affect non-target arthropods common for bean fields as well as the main pests of beans, namely aphids and miner flies.

ecology↗