Korshunova, N. | bioRxiv Science

bioRxiv2024

PAX proteins in silico prediction in Lissachatina fulica

Prediction of snail PAX group proteins in Lisachatina fulica was performed. For this this purpose, homologous sequences to already known PAX proteins of Cephalopoda, Gastropoda and Bivalvia molluscs were predicted. The presence of certain domains (Paired box, Homeodomain and octapeptide) in the structure of the primary predicted sequences of Lisachatina fulica proteins from the GigaDB was analyzed. Also, to confirm that the sequences found belong to the PAX family proteins, their secondary and 3D structures were predicted, and their DNA-binding capacity was compared to the control Paired box domain using molecular docking. As a result, 8 proteins of Lisachatina fulica containing PAX characteristic sequences were identified. Based on the results of domain structure search and phylogenetic analysis, the proteins were categorized into 5 subfamilies of PAX proteins, namely I, III, IV, {beta}, and Pox-Neuro. The result provides a starting point for the search for the functional role of PAX proteins in the Lisachatina fulica.

evolutionary biology↗

bioRxiv2022

Synergizing algorithmic design, photoclick chemistry and multi-material volumetric printing for accelerating complex shape engineering

Accelerating the designing and manufacturing of complex shapes has been a driving factor of modern industrialization. This has led to numerous advances in computational design and modeling and novel additive manufacturing (AM) techniques that can create complex shapes for bespoke applications. By combining a new coding-based design approach with high-throughput volumetric printing, we envision a new approach to transform the way we design and fabricate complex shapes. Here, we demonstrate an algorithmic voxel-based approach, which can rapidly generate and analyze porous structures, auxetic meshes and cylinders, or perfusable constructs. We use this design scheme in conjunction with new approaches for multi-material volumetric printing based on thiol-ene photoclick chemistry to rapidly fabricate complex heterogeneous structures. Collectively, the new design and fabrication technique we demonstrate can be used across a wide-spectrum of products such as actuators, biomedical implants and grafts, or tissue and disease models. TeaserA new scheme of rapidly designing and printing complex multi-material structures for implant and tissue graft applications.

bioengineering↗