Biology subjects

Kok-Gan Chan

Publications and source records attributed to Kok-Gan Chan.

2 recordsLinked to original sources

bioRxiv2015

Discovery of Pandoraea pnomenusa RB38 N-acyl homoserine Lactone Synthase (PpnI) and its Complete Genome Sequence Analysis

In this study, we sequenced the genome of P. pnomenusa RB38 and reported the finding of a pair of cognate luxI/R homologs which we firstly coined as ppnI, which is found adjacent to a luxR homolog, ppnR. An additional orphan luxR homolog, ppnR2 was also discovered. Multiple sequence alignment revealed that PpnI is a distinct cluster of AHL synthase compared to those of its nearest phylogenetic neighbor, Burkholderia spp. When expressed heterologously and analysed using high resolution tandem mass spectrometry, PpnI directs the synthesis of N-octanoylhomoserine lactone (C8-HSL). To our knowledge, this is the first documentation of the luxI/R homologs of the genus of Pandoraea.

Microbiology↗

bioRxiv2015

Genome analysis leads to discovery of Quorum Sensing Genes in Cedecea neteri

We have identified a strain of C. neteri SSMD04 isolated from pickled mackerel sashimi that produced N-acyl-homoserine lactone (AHL) type quorum sensing (QS) activity. Tandem mass sspectrometry revealed that C. neteri SSMD04 produced N-butyryl-homoserine lactone (C4-HSL). We identified a pair of luxIR homologues in this genome that shares the highest similarity with croIR from Citrobacter rodentium. The AHL synthase, which we named it as cneI (636 bp) and at 8bp distance from cneI is a sequence encoding a hypothetical protein, potentially the cognate receptor, a luxR homologue which we named it as cneR. We also found an orphan luxR in this isolate. To our knowledge, this is the first report on the AHL production activity in C. neteri, discovery of its luxI/R homologues and the orphan receptor.

Microbiology↗