Biology subjects

Koïta, K.

Publications and source records attributed to Koïta, K..

2 recordsLinked to original sources

bioRxiv2023

Culturable approach to rice-root associated bacteria in Burkina Faso: diversity, plant growth-promoting rhizobacteria properties and cross-comparison with metabarcoding data.

Plant-associated bacteria are essential partners in plant health and development. In addition to taking advantage of the rapid advances recently achieved in high-throughput sequencing approaches, studies on plant-microbiome interactions require experiments with culturable bacteria. A study on the rice root microbiome was recently initiated in Burkina Faso. As a follow up, the aim of the present study was to develop a collection of corresponding rice root-associated bacteria covering maximum diversity so as, to be able to assess the diversity of the collection based on the culture medium used, and to describe the taxonomy, phenotype and abundance of selected isolates in the rice microbiome. More than 3,000 isolates were obtained using five culture media (TSA, NGN, NFb, PCAT, Baz). The 16S rRNA fragment sequencing of 1,013 selected working collection isolates showed that our working collection covered four bacterial phyla (Proteobacteria, Firmicutes, Actinobacteria and Bacteroidetes) and represented 33% of the previously described diversity of the rice root microbiome at the order level. Phenotypic in vitro analysis of the plant growth promoting capacity of the isolates revealed an overall ammonium production and auxin biosynthesis capacity, while siderophore production and phosphate solubilisation were enriched in Burkholderia, Ralstonia, Acinetobacter and Pseudomonas species. Of 45 representative isolates screened for growth promotion on seedlings of two rice cultivars, five showed an ability to improve the growth of both cultivars, while five others were effective on only one cultivar. The best results were obtained with Pseudomonas taiwanensis ABIP 2315 and Azorhizobium caulinodans ABIP 1219, which increased seedling growth by 158% and 47%, respectively. Among the 14 best performing isolates, eight appeared to be abundant in the rice root microbiome dataset from previous study. The findings of this research contribute to the functional description of rice root-associated bacteria and their potential importance for plants by providing, for the first time, insight into their prevalence in the rice root microbiome.

microbiology↗

bioRxiv2022

Quantification of the diversity sampling bias resulting from rice root bacterial isolation on popular and nitrogen-free culture media, using 16S amplicon barcoding

Culturing bacteria from plant material is well known to introduce a strong bias compared to the real diversity present in the original samples. This bias is related to cultivability of bacteria, the chemical composition of media and culture conditions. The bias of recovery is often observed but was never quantified on different media using an amplicon barcoding approach comparing plant microbiota DNA extractions versus DNA extracted from serial dilutions of the same plant tissues grown on bacterial culture media. In this study, we i) quantified the culturing diversity bias using 16S amplicon barcode sequencing by comparing a culture-dependent approach (CDA) on rice roots on four popular bacterial media (Tryptone Soybean Agar-TSA-at two concentrations, 10% and 50%; a plant-based media with rice flour; Norris Glucose Nitrogen Free Medium-NGN; and Nitrogen Free -NFb) versus a culture-independent approach (CIA) assessed from DNA extracted directly on root and rhizosphere samples; ii) assessed enriched and missing taxa detected on the different media; iii) use biostatistics functional predictions to predict which metabolic profiles are enriched in the CDA and CIA. A comparative analysis of the two approaches revealed that among the 22 phyla present in the microbiota of the studied rice root samples, only five were present on the culture media approach (Proteobacteria, Firmicutes, Bacteroidetes, Actinobacteria, Verrucomicrobia). The Proteobacteria phylum was the most abundant in all cultured media samples, showing a high enrichment of gamma-Proteobacteria. The diversity of the combined culture media represented about 1/3 of the diversity of the total microbiota, and its genus diversity and frequency was documented. The functional prediction tool (PiCrust2) detected an enrichment of nitrogenase enzyme in bacterial taxa sampled from Nitrogen-free media, validating its predictive capacity. Further functional predictions also showed that the CDA missed mostly anaerobic, methylotrophic, methanotrophic and photosynthetic bacteria compared to the culture independent approach, delivering valuable insights to design ad-hoc culture media and conditions to increase cultivability of the rice-associated microbiota.

microbiology↗