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Koca, J.

Publications and source records attributed to Koca, J..

4 recordsLinked to original sources

Uncovering of Cytochrome P450 Anatomy by SecStrAnnotator

Protein structural families are groups of homologous proteins defined by the organization of secondary structure elements (SSEs). Nowadays, many families contain vast numbers of structures, and the SSEs can help to orient within them. Communities around specific protein families have even developed specialized SSE annotations, always assigning the same name to the equivalent SSEs in homologous proteins. A detailed analysis of the groups of equivalent SSEs provides an overview of the studied family and enriches the analysis of any particular protein at hand. We developed a workflow for the analysis of the secondary structure anatomy of a protein family. We applied this analysis to the model family of cytochromes P450 (CYPs) - a family of important biotransformation enzymes with a community-wide used SSE annotation. We report the occurrence, typical length and amino acid sequence for the equivalent SSE groups, the conservation/variability of these properties and relationship to the substrate recognition sites. We also suggest a generic residue numbering scheme for the CYP family. Comparing the bacterial and eukaryotic part of the family highlights the significant differences and reveals an anomalous group of bacterial CYPs with some typically eukaryotic features. Application of our workflow to other families could produce equally surprising findings.

bioinformatics↗

NAChRDB: A Web Resource of Structure-Function Annotations to Unravel the Allostery of Nicotinic Acetylcholine Receptors

SummaryDue to their paramount importance, near-ubiquitous presence, and complex nature, nicotinic acetylcholine receptors (nAChRs) have remained the focus of intensive research for over 50 years. The vast amount of knowledge accumulated on the topic has become extremely difficult to navigate. NAChRDB addresses this challenge by providing web-based, real-time access to curated residue-level functional annotations of neuromuscular nAChRs with interactive 3D visualization and sequence alignment. NAChRDB provides systematic access to experimental observations and predictions from computational studies reported in the literature or performed specifically to complement current knowledge, which allows new findings to be interpreted in a more holistic context, both from a structural and a functional perspective. NAChRDB aims to serve as an invaluable resource for identifying gaps in knowledge and for guiding discovery through structural and molecular biology experiments, especially when exploring the allosteric mechanisms underlying neuromuscular nAChR function and pathology. Availability and ImplementationNAChRDB is freely available online, with a self-explanatory interface and useful tool tips (https://crocodile.ncbr.muni.cz/Apps/NAChRDB/). No installation or user registration is required. NAChRDB content is stored in .json format, queried using Python, and rendered in browser using Javascript and WebGL (LiteMol). NAChRDB is highly responsive and accessible through any modern Internet browser on desktop and mobile devices. Contactjkoca@ceitec.cz Supplementary informationSupplementary data are available online.

bioinformatics↗

The evolutionary advantage of toxin production among cyanobacteria, the oldest known organisms on Earth

Cyanobacteria produce toxic secondary metabolites for reasons hitherto unclear. Using a phylogenetic approach that accounts for the high complexity of biosynthetic gene clusters (full or partial inversions, variable length, different number of genes, non-orthologues), we analyzed the sequences of 76 biosynthetic gene clusters covering 19 cyanotoxins. The phylogenetic tree of biosynthetic gene clusters branches first according to the bioactivity of the toxic metabolite (molecular target in another organism), then according to the chemical class and chemical structure of the secondary metabolite, and finally according to the organism and area of origin. The bioactivity of a toxic metabolite can be deduced directly from the nucleotide sequence of the biosynthetic gene cluster, without needing to examine the enzymes themselves or to measure expression levels. Bioactivity may have been the primary driving force behind the diversity of secondary metabolism in cyanobacteria. This genetic machinery evolved to facilitate three specific survival strategies acting separately or in tandem, with dominant cyanobacteria possessing the genetic machinery to support all three strategies. Transmembrane (direct) toxicity targeting ion channels, intracellular (indirect) toxicity targeting cell-cycle regulation, and digestion inhibition targeting proteases may have provided the survival advantage underpinning the evolutionary success of both cyanobacteria and their early symbiotic hosts.

bioinformatics↗

Charge-perturbation dynamics - a new avenue towards in silico protein folding

Molecular dynamics (MD) has greatly contributed to understanding and predicting the way proteins fold. However, the time-scale and complexity of folding are not accessible via classical MD. Furthermore, efficient folding pipelines involving enhanced MD techniques are not routinely accessible. We aimed to determine whether perturbing the electrostatic component of the MD force field can help expedite folding simulations. We developed charge-perturbation dynamics (CPD), an MD-based simulation approach that involves periodically perturbing the atomic charges to values non-native to the MD force field. CPD obtains suitable sampling via multiple iterations in which a classical MD segment (with native charges) is followed by a very short segment of perturbed MD (using the same force field and conditions, but with non-native charges); subsequently, partially folded intermediates are refined via a longer segment of classical MD. Among the partially folded structures from low-energy regions of the free-energy landscape sampled, the lowest-energy conformer with high root-mean-square deviation to the starting structure and low radius of gyration is defined as the folded structure. Upon benchmark testing, we found that medium-length peptides such as an alanine-based pentadecapeptide, an amyloid-{beta} peptide, and the tryptophan-cage mini-protein can fold starting from their extended linear structure in under 45 ns of CPD (total simulation time), versus over 100 ns of classical MD. CPD not only achieved folding close to the desired conformation but also sampled key intermediates along the folding pathway without prior knowledge of the folding mechanism or final folded structure. Our findings confirmed that perturbing the electrostatic component of the classical MD force field can help expedite folding simulations without changing the MD algorithm or using expensive computing architectures. CPD can be employed to probe the folding dynamics of known, putative, or planned peptides, as well as to improve sampling in more advanced simulations or to guide further experiments.\n\nAuthor summaryFolding represents the process by which proteins assemble into biologically active conformations. While computational techniques such as molecular dynamics (MD) have provided invaluable insight into protein folding, efficient folding pipelines are not routinely accessible. In MD, the behavior of the studied molecule is simulated under the concerted action of multiple forces described by mathematical functions employing optimized parameters. Using non-native parameters effectively perturbs the MD force field. We show that this can be exploited to help expedite folding simulations. Specifically, we developed charge-perturbation dynamics (CPD), an MD-based simulation approach that involves periodically perturbing the force field by using non-native atomic charges. For folding medium-length peptides such as the tryptophan-cage mini-protein starting from the extended linear structure, CPD is much faster than other MD-based approaches while using the same software, hardware, and know-how required for running classical MD simulations. Furthermore, CPD not only achieves folding close to the desired conformation but also samples key intermediates along the folding pathway without prior knowledge of the folding mechanism or final folded structure. CPD can be employed to probe the folding dynamics of known, putative, or planned peptides, as well as to generate different conformations that can guide further experiments or more advanced simulations.

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