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Kayejo, V. G.

Publications and source records attributed to Kayejo, V. G..

2 recordsLinked to original sources

Vav2 is a master regulator of repair against bacterial pore-forming toxins.

Despite antibiotic therapy, 25-35% of patients with necrotizing soft tissue infections (NSTIs) die. The etiologic agents for NSTIs include Streptococcus pyogenes and Clostridium perfringens, which secrete the cholesterol-dependent cytolysins (CDCs) streptolysin O and perfringolysin O to disrupt cell membranes. While cells resist this damage by activating Ca2+-dependent repair pathways, including MEK-dependent microvesicle shedding, dysferlin-mediated patch repair, and annexin-mediated membrane clogging, the upstream regulators of these responses have remained elusive. Here, we demonstrated that the Rac GEF Vav2 accounts for almost all of Ca2+-dependent repair against CDCs. Inhibiting or knocking down Vav2 sensitized multiple cell types to CDCs, whereas blocking other Rac GEFs did not. Mechanistically, Vav2 triggered the critical MLK3-MEK-dependent repair pathway. MEK activation rescued repair in Vav2-inhibited cells. Blocking dysferlin or annexins failed to increase damage beyond Vav2 inhibition, suggesting Vav2 coordinates multiple repair pathways. Thus, Vav2 controls multiple Ca2+-activated repair pathways that protect cells from CDCs produced during NSTIs. TeaserThis one protein accounts for almost all Ca2+-dependent repair by activating at least 3 distinct pathways.

cell biology↗

The fungal peptide toxin candidalysin induces distinct membrane repair mechanisms compared to bacterial pore-forming toxins

The common fungal pathogen, Candida albicans, relies on the pore-forming toxin candidalysin to damage host cells. Cells counteract pore-forming toxins by Ca2+-dependent mechanisms, such as microvesicle shedding and annexin recruitment to resist cholesterol-dependent cytolysins like streptolysin O (SLO), or annexin involvement and patch repair in the case of aerolysin. However, the specific Ca2+-dependent repair pathways engaged in response to candidalysin remain poorly understood. Here, we determined the involvement of different Ca2+-dependent repair mechanisms to candidalysin and compared responses to SLO and aerolysin using flow cytometry and high-resolution microscopy. We report that candidalysin triggered Ca2+-dependent repair, but patch repair and ceramide failed to provide significant protection. MEK-dependent repair and annexins A1, A2 and A6 contributed partially to repairing damage caused by candidalysin. However, annexin translocation after candidalysin challenge was delayed compared to SLO or aerolysin challenge. Surprisingly, extracellular Cl- improved cell survival after candidalysin challenge, but not after challenge with SLO or aerolysin. Finally, we found that candidalysin is removed via extracellular vesicle shedding. These findings reveal that Ca2+-dependent microvesicle shedding protects cells from candidalysin and can be engaged by multiple molecular mechanisms during membrane repair. O_FIG O_LINKSMALLFIG WIDTH=200 HEIGHT=142 SRC="FIGDIR/small/653080v2_figa1.gif" ALT="Figure 1"> View larger version (43K): org.highwire.dtl.DTLVardef@433b3aorg.highwire.dtl.DTLVardef@1e583f3org.highwire.dtl.DTLVardef@139f086org.highwire.dtl.DTLVardef@de07b0_HPS_FORMAT_FIGEXP M_FIG O_FLOATNOGraphical Abstract.C_FLOATNO Candidalysin is resisted by distinct repair mechanisms compared to bacterial PFTs.After pore formation and membrane damage by each toxin, multiple repair pathways are triggered downstream of Ca{superscript 2} flux. Candidalysin induces a protective Cl- influx and activates MEK-dependent repair, which contributes to cell protection. Annexin translocation occurs slowly and provides minor protection, while patch repair is ineffective. In contrast, aerolysin does not benefit from Cl- influx or MEK protection. Aerolysin triggers moderate annexin translocation and relies primarily on patch repair as the main protective mechanism. Streptolysin O elicits rapid annexin translocation and activates MEK signaling, both of which contribute to robust protection. Patch repair plays only a minor protective role against SLO. The figure was created using BioRender. C_FIG

cell biology↗