Biology subjects

Katagiri, Y.

Publications and source records attributed to Katagiri, Y..

3 recordsLinked to original sources

bioRxiv2022

A Novel Cytoskeletal Action of Xylosides

Proteoglycan glycosaminoglycan (GAG) chains are attached to a serine residue in the protein through a linkage series of sugars, the first of which is xylose. Xylosides are chemicals which compete with the xylose at the enzyme xylosyl transferase to prevent the attachment of GAG chains to proteins. These compounds have been employed at concentrations in the millimolar range as tools to study the role of GAG chains in proteoglycan function. In the course of our studies with xylosides, we conducted a dose-response curve for xyloside actions on neural cells. To our surprise, we found that concentrations of xylosides in the nanomolar to micromolar range had major effects on cell morphology. These effects are due to changes in cytoskeletal dynamics. Concentrations of xylosides which were effective in altering morphology did not alter GAG chain synthesis rates, nor did they produce any changes in gene expression as determined by RNAseq of treated cells. These observations support a novel action of xylosides on neuronal cells.

cell biology↗

bioRxiv2021

Serum hormone levels in infertile women between conceived with and without hormone replacement therapy: Safety of estradiol and progesterone replacement therapy in frozen-thawed embryo transfer cycles for breast cancer survivors.

ObjectiveRecent advances in cancer treatment and reproductive medicine have made the post-treatment quality of life an important concern for cancer survivors. We aimed to evaluate the safety of sex hormone (estradiol and progesterone) replacement therapy (HRT) in women who conceived by assisted reproductive technology (ART) with hormone receptor-positive breast cancer. MethodsWe measured serum E2 and P4 levels at 4-10 weeks of gestation in women who conceived naturally or after timed intercourse or intrauterine insemination for infertility without HRT for luteal support (non-HR group; n=135). We conducted a retrospective comparison of the values from the non-HR group with those of women who conceived by ART with HRT for infertility (HR group; n=75). ResultsSerum E2 levels were significantly higher in the non-HR group than in the HR group at 5, 6, and 8 weeks of gestation. Similarly, serum P4 levels were significantly higher in the non-HR group than in the HR group at 4, 5, and 6 weeks of gestation. ConclusionsThis study suggests that in cancer reproductive medicine for hormone-dependent breast cancer survivors, HRT administered during the first trimester of a pregnancy after primary disease treatment may not increase the sex hormone levels to levels above those seen in spontaneous pregnancy.

cancer biology↗

bioRxiv2018

The lipid phosphatase-like protein PLPPR1 increases cell adhesion by modulating RhoA/Rac1 activity

PLPPR1 alters cytoskeletal dynamics to influence cell adhesion by modulating RhoA and Rac1 activation through its association with RhoGDI, enabling PLPPR1 to impede the inhibitory activity of CSPGs and LPA.\n\nABSTRACTPhospholipid Phosphatase-Related Protein Type 1 (PLPPR1) is a member of a family of lipid phosphatase related proteins, integral membrane proteins characterized by six transmembrane domains. This family of proteins is enriched in the brain and recent data indicate potential pleiotropic functions in several different contexts. An inherent ability of this family of proteins is to induce membrane protrusions, and we have previously reported that members of this family may act in concert. However, the function of PLPPR1 is not yet understood. Here, we report that exogenous expression of PLPPR1 increases cell adhesion to the ECM substrate by altering cytoskeletal dynamics and modulating RhoA and Rac1 activity through association with RhoGDI, enabling PLPPR1 to overcome the inhibitory activity of CSPGs and LPA. Together, these results establish a novel signaling pathway for PLPPR1 protein.

neuroscience↗