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Kanukollu, S.

Publications and source records attributed to Kanukollu, S..

2 recordsLinked to original sources
bioRxiv2025

STREAMLINING HISTOPATHOLOGY: INSTAPRESERVE TISSUESAFE A SINGLE-STEP FIXATIVE THAT IMPROVES TISSUE QUALITY AND WORKFLOW EFFICIENCY

In clinical pathology, tissue processing is crucial in generating a valuable report for faster diagnosis. Formalin-fixed paraffin-embedded tissues are the standard specimens for clinical and molecular analyses. Though the demand persists for faster diagnosis, neutral buffered formalin (NBF) -based fixation and processing have not been replaced with a simple, faster, and user-friendly method. The alcohol-based fixatives were evaluated as an alternative to formalin, yet none have replaced formalin for various reasons. InstaPRESERVE Tissuesafe is an alcohol-based, biodegradable, and user-friendly fixative solution. The clinical specimens are well preserved and suitable for histopathology, genomics, and proteomics studies. The efficacy of instaPRESERVE Tissuesafe with ethanol or isopropyl alcohol and a modified tissue processing method without alcohol dehydration steps was studied. The pigs organs, brain, and liver tissues were fixed with NBF and InstaPRESERVE Tissuesafe solutions (ethanol/isopropyl alcohol). Based on the alcohol content of instaPRESERVE Tissuesafe, the dehydration step(s) were skipped during tissue processing. Serial sections from fixed tissues were processed and stained with H&E. The intactness of the sections with instaPRESERVE Tissuesafe fixation was comparable to that of NBF fixation. No autolysis/ decomposition was observed with InstaPRESERVE Tissuesafe (both) and NBF. The H&E staining scoring was satisfactory with well-preserved cell boundaries and organelles in both liver and brain tissue sections. The key factors of any fixative are its penetration, temperature, and time taken for fixation. In this study, the penetration rate of InstaPRESERVE Tissuesafe with either ethanol or isopropyl alcohol was the same as NBF within 6 to 12 hrs. The results indicated that increased concentration of alcohol in instaPRESERVE Tissuesafe did not affect the histology, and overall quality of the tissue sections processed was comparable to formalin fixation. The cytoplasmic and nuclear details, erythrocyte integrity, and lymphocytic appearance were well maintained. The time was reduced to 6 hrs from 9 hrs of the standard method. This study has addressed the modification of tissue processing with reduced time in histopathology with instaPRESERVE Tissuesafe. The outcome of this study has shown that instaPRESERVE Tissuesafe may be an alternative to formalin and helps in faster diagnosis.

pathology↗
bioRxiv2025

Biobanking of Biological Samples at Room Temperature

Biospecimens have become an integral part of life science research, pharma and drug development. There is increased demand for biospecimens from patients with assured quality of collection, processing, and storage. Establishing a biobank with high-quality biospecimens is an expensive aspect due to the requirement of costly freezers and liquid nitrogen facilities. Many studies have explored various storage reagents for biological samples. Still, the need of the hour is the one that can preserve biological samples at ambient room temperature and suitable for diagnosis and research. InstaPRESERVE is an eco/user-friendly solution with long-term sustainable alternative for freezer or liquid nitrogen systems for biobanking. Blood and saliva specimens were collected and stored in InstaPRESERVE at room temperature and conventional storage conditions. The genomics and proteomics analysis of the DNA, RNA, and protein extracted from the -80{degrees}C and InstaPRESERVE stored specimens and fresh specimens indicated that the specimens were well-conserved at room temperature compared to frozen specimens. Innovative technologies and approaches are being evaluated in biobanks for sample integrity, reducing collection, processing, and storage costs, increasing speed and throughput, and reducing energy expenditure. Preserving the biospecimens in InstaPRESERVE at room temperature eliminates expensive storage systems, cold-chain during transportation, and pre-processing requirements. The qualitative and quantitative results of DNA, RNA, and protein ensured stabilized storage for a longer period. The use of InstaPRESERVE as a specimen storage medium is emerging as an alternative in biobanking for specimen storage at room temperature. The existing and proposed biobanks may consider employing InstaPRESERVE as a reliable resource for biospecimens storage.

molecular biology↗