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Biology subjects

Kaminska, J.

Publications and source records attributed to Kaminska, J..

2 recordsLinked to original sources

Visual perception and workload of office workers in various acoustic conditions

Noise in the office work environment can negatively affect workers cognitive performance, number of errors made and comfort. The aim of the study was to determine the effects of various types of acoustic conditions in the mental work environment on visual perception (eye-tracking parameters) and workload. Method: In the experiment a group of 39 people aged 20 to 34 was asked to perform two eye-tracking tests (Perceptiveness and Speed Tests (PTs) and to read the text of a fictional biography, and then to answer questions about the reading). Mental workload was evaluated in each condition using NASA TLX questionnaire. The tests were performed in various acoustic conditions: variant W1 - no presentation of acoustic stimuli, variant W2 - sounds of office equipment, variant W3 - sounds of office equipment with quiet conversation in native language, variant W4 - sounds of office equipment with loud conversation in native language, variant W5 - filtered pink noise. In variants from W2 to W5 the equivalent sound level A was 55 dB. Results: The assessment of work efficiency in the reading test indicates the existence of statistically significant differences. The most errors were made during mental work with the audible sounds of office equipment with a loud conversation (Variant W4) and during mental work performed with audible filtered pink noise (W5). While reading the text, different acoustic conditions do not differentiate statistically significantly visual perception described by eye-tracking parameters. In turn, in the PTs test, statistically significant differences between the variants were found in the Digit test (average blink duration) and the Fraction test (average blink duration, average fixation duration and saccades frequency parameters). In conclusion, visual perception depends on the type of noise. Acoustic factors aggect workers cognitive functions, mostly in more difficult tasks.

bioengineering↗

Intracellular level of S. cerevisiae Rad51 is regulated via proteolysis in a SUMO- and Ubiquitin-dependent manner

Among various DNA lesions, the DNA double-strand breaks are particularly deleterious; especially, when an error-free repair pathway is unavailable, and the cell takes the risk of using the error-prone recombination pathways to repair the DNA breaks, resume the cell cycle, and continue growth. The latter comes at the expense of decreased well-being of the cells due to genome rearrangements. One of the major players involved in recombinational repair of DNA damage is Rad51 recombinase, a protein responsible for presynaptic complex formation. We previously noticed that the level of this protein is strongly increased when illegitimate recombination is engaged in repair. The regulation of Rad51 protein turnover is not known; therefore, we decided to look closer at this issue because we expect that an excessively high level of Rad51 may lead to genome instability. Here we show that the level of Rad51 is regulated via the ubiquitin-dependent proteolytic pathway. The ubiquitination of Rad51 depends on multiple E3 enzymes, including SUMO-targeted ubiquitin ligases. We also demonstrate that Rad51 can be modified by both ubiquitin and SUMO. Moreover, these modifications may lead to opposite effects. Ubiquitin-dependent degradation depends on Rad6, Rad18, Slx8, Dia2 and the anaphase-promoting complex. Rsp5-dependent ubiquitination leads to Rad51 stabilization.

genetics↗