Biology subjects

Jutoy, J.

Publications and source records attributed to Jutoy, J..

2 recordsLinked to original sources

bioRxiv2025

Agarose Stamped Device: A simple but effective fixation method for Zebrafish Larvae through Customizable 3D Printed Molds for Danio rerio (Zebrafish)

Precise immobilization of zebrafish larvae is critical for high-resolution neural imaging and behavioral studies, yet conventional methods are often labor-intensive, low-throughput, and can induce stress. To address these challenges, we introduce the Agarose Stamped Device (ASD), a novel platform enabling efficient and gentle immobilization of zebrafish larvae for high-resolution imaging and behavioral assays. By employing a stamping approach to mold an array of consistent agarose wells, the ASD enables rapid, parallel, and reproducible positioning of larvae in a standardized orientation while minimizing stress and preserving viability. We validated ASD performance by comparing larval survival, heart rate, and imaging stability to conventional agarose embedding, finding no adverse effects on larval physiology and significantly enhanced imaging throughput and data consistency. Demonstrations in neuroscience and behavioral experiments underscore the devices versatility for diverse in vivo studies. The ASD offers a simple, cost-effective, and powerful tool for advancing zebrafish-based neuroscience and behavioral research.

bioengineering↗

bioRxiv2024

Larva in the loop, a closed loop machine interface system for Danio rerio larvae

The optokinetic response (OKR) in Zebrafish (Danio Rerio) had been characterized for its robust response to visual stimuli. Expanding on these works, we developed a novel closed loop control schema to drive a robot utilizing the OKR of Zebrafish larvae. Our system keeps the body of a larva constrained via a novel agarose mold holder that allows for eye movement and vision. The larva is then put under a microscope camera and processed through computer vision to track its eyes via ellipse fitting. Relative eye angle data is then parsed through an algorithm and used to send movement signals to a robot on a lined track. Simultaneously, the robot returns its relative position with respect to the line and converts that information into an OKR stimulation animation which is displayed on an LCD screen in the ventral plane of the larva, thus closing the loop. Through this work we show the capability of larvae OKR to keep a robot on a linear track after an initial oblique entrance to the line. This work displays the potential of our system and how it can pave the way to a Zebrafish Brain-Machine Interface.

animal behavior and cognition↗