Julkowski, K. | bioRxiv Science

bioRxiv2025

Partner cues and individual variation underlie sex-reversed parental care in poison frogs

Flexible parental care strategies are widespread in nature and factor into conflict between the sexes and the realization of sex roles. While adaptive explanations abound, the mechanisms underlying flexible sex-reversal of care are less clear. We enlist a biparental frog (Ranitomeya imitator) with flexible parental care to investigate the extrinsic and intrinsic mechanisms underpinning parental decisions. Using mate removal experiments in the laboratory, we show that members of the primary caregiving sex (males) show less variation than the flexible sex (females) in their propensity to provide care, and that care propensity in females is affected by extrinsic partner cues as well as individual variability. Indeed, individual repeatability in parental effort is high in both typically caregiving and flexible parents. To investigate the underpinnings of differences in care propensity, we sequenced RNA from whole brains of caregiving and non-caregiving frogs of both sexes. While actively caregiving females showed minimal differential gene expression compared to actively caregiving males, females that failed to provide care showed distinct patterns of gene expression. Our findings offer an initial glimpse into the environmental and genetic regulation of individual variation in sex-reversed parental care.

animal behavior and cognition↗

bioRxiv2025

Discrete genetic modules underlie divergent reproductive strategies in three-spined stickleback

A central challenge in biology is to understand how complex behaviors evolve. Given their importance to fitness, complex behavioral traits often evolve as an integrated package, but it is unclear whether suites of traits evolve through a few pleiotropic genetic changes, each affecting many behaviors, or by accumulating several changes that, when combined, give rise to an entire package of correlated traits. Typically, three-spined stickleback exhibit paternal care, a behavior that characterizes the entire Gasterosteidae family. However, an unusual "white" three-spined stickleback ecotype exhibits a suite of traits associated with the evolutionary loss of paternal care. In the white ecotype, males disperse embryos from their nests rather than care for them, build loose nests, exhibit high rates of courtship, and are relatively small in body size. These behavioral differences are apparent in stickleback reared in a common garden environment, suggesting the differences have a heritable basis. In an F2 intercross, we show that these traits are genetically uncorrelated and map to different genomic regions, suggesting that components of the white reproductive strategy segregate independently and evolved through the addition of multiple genetic changes. These results contribute to the growing body of evidence that the behavioral diversity observed in nature may often evolve by accumulating and combining alleles, each with modular effects, and show that this principle applies to a suite of behavioral traits that together form an integrated and adaptive strategy.

evolutionary biology↗