Biology subjects

Jansson, C.

Publications and source records attributed to Jansson, C..

3 recordsLinked to original sources

bioRxiv2025

RhizoGrid: Illuminating Spatial Rhizosphere Dynamics

The rhizosphere microbiome directly influences plant health and acclimation to extreme environments, yet plant-microbe interactions in the rhizosphere have proven complex and difficult to study. We present RhizoGrid, a new methodological framework that integrates a 3D-printed pot structure with spatial measurements of root structure, metabollite and taxonomy to detect links between metabolites and microbes along a soil-grown root system. The RhizoGrid identifies microhabitats hidden belowground. Using the food, forage, and bioenergy crop sorghum, we showcase how the RhizoGrid opens new frontiers for exploring the heterogeneity and complexity of interactions between root exudates and microbes in the rhizosphere.

systems biology↗

bioRxiv2024

Spatial transcriptomics exploration of the primary neuroblastoma microenvironment unveils novel paracrine interactions

High-risk neuroblastomas exhibit a high degree of intratumoral heterogeneity. Single-cell RNA sequencing has greatly improved our understanding of these tumors, but the method lacks cellular tissue context and spatial information about local signaling dynamics. To address this gap, we profiled untreated and chemotherapy-treated high-risk neuroblastomas from 2 patients using spatial transcriptomics. We confirmed the transcriptional and cellular heterogeneous nature of the neuroblastoma microenvironment and identified several unique spatial niches and patterns. In one of the treated tumors, a spatially constrained cluster of undifferentiated and 11p-gained cancer cells was identified, surrounded by a rim of macrophages. A signaling interaction between the chemokine CCL18 and its receptor PITPNM3 was predicted between these cells and we experimentally demonstrated that CCL18 increases neuroblastoma cell migration. In the other tumor, we identified a stromal cluster with high transcriptional similarity to the adrenal cortex. These adrenocortical-like cells expressed the ALK ligand ALKAL2 and were predicted to communicate with neighboring ALK expressing cancer cells. We demonstrated a unique developmental pattern of adrenal medulla-specific expression of ALK and adrenocortical-specific expression of ALKAL2, suggesting a role of these signaling interactions in neuroblastoma carcinogenesis.

cancer biology↗

bioRxiv2020

Altered parasitism of a butterfly assemblage associated with a range-expanding species

AimBiotic interactions are an important factor structuring ecological communities but data scarcity limits our understanding of the impact of their response to climate and land use changes on communities. We studied the impact of a change in species assemblage on biotic interactions in a community of closely-related butterflies. Specifically, we examined the impact of the recent range expansion of Araschnia levana on the resident species, with a particular focus on natural enemies, parasitoids, shared with other butterfly species in the assemblage. LocationSweden. Time periodTwo years (2017-2018). Major taxa studiedNettle-feeding butterflies (Aglais urticae, Aglais io, Araschnia levana, and Vanessa atalanta) and their parasitoids. MethodsWe assessed parasitism in 6777 butterfly larvae sampled in the field from 19 sites distributed along a 500 km latitudinal gradient, and every two weeks throughout species reproductive seasons. We identified the parasitoid complex of each butterfly species and their overlap, and analysed how parasitism rates were affected by species assemblage, variations in abundance, time, and the arrival of A. levana. ResultsParasitoids caused high mortality, with substantial overlap across the four host species. The composition of the host community influenced parasitism rates and this effect was specific to each species. In particular, the rate of parasitism in resident species was comparatively higher at sites where A. levana has been established for longer. Main conclusionsParasitoid pressure is a significant source of mortality in the nettle-feeding butterfly community studied. Variations in butterfly species assemblages are associated with substantial variations in rates of parasitism. This is likely to affect the population dynamics of their butterfly host species, and, potentially, the larger number of species with which they interact.

ecology↗