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Jan, S.

Publications and source records attributed to Jan, S..

2 recordsLinked to original sources

Response of Various Concentrations of Indole Acetic Acid (IAA) to Morphological Attributes in Different Plum (Prunus domestica L.) Curltivars

AbstractsThis study was conducted to evaluate the effects of indole-3-acetic acid (IAA) on budwood treated with different concentrations of indole acetic acid i.e. ((300, 600, 900 and 1200 ppm) of three plum varieties (Fazle Manani, Santa Rosa and Red Beauty) in respect of various morphological parameters e.g. days to sprouting, plant height, number of branches, percent sprouting, number of leaves, budling diameter and internode length. Results revealed that different varieties of plum had different responses to the applied concentrations in different plum cultivars. Cultivar Fazle manani showed significant decline in days to sprouting (at 300 ppm dose), plant height (at 600 dose), percent sprouting and budling diameter (at all concentrations) while increase in number of branches (1200 dose), number of leaves (all concentrations except 600 ppm), and internode length (at 300 ppm and 900 ppm concentrations). Days to sprouting in cultivar Santa rosa were non-significantly affected while plant height and internode length were significantly reduced at all concentrations particularly at 300 ppm. Number of branches, number of leaves and percent sprouting increased significantly at 300 ppm and 900 ppm concentrations. Cultivar red beauty exhibited significant decline in days to sprouting at 600 ppm and 900 ppm concentrations while elevated plant height at 900 concentrations. Number of branches, percent sprouting, number of leaves and budling diameter in Red Beauty were inhibited by all concentrations. However, internode length was increased significantly at 300 ppm, 600 ppm and 1200 ppm concentrations but decreased at 900 ppm concentrations. The study revealed that the most effective dose was 300 ppm. These findings demonstrate the differential sensitivity of plum cultivars to IAA and highlight its potential for regulating their growth and morphology.

physiology↗

Auranofin resistance in Toxoplasma gondii decreases the accumulation of reactive oxygen species but does not target parasite thioredoxin reductase

Auranofin, a reprofiled FDA-approved drug originally designed to treat rheumatoid arthritis, has emerged as a promising anti-parasitic drug. It induces the accumulation of reactive oxygen species (ROS) in parasites, including Toxoplasma gondii. We generated auranofin resistant T. gondii lines through chemical mutagenesis in order to identify the molecular target of this drug. Resistant clones were confirmed with a competition assay using wild-type T. gondii expressing yellow fluorescence protein (YFP) as a reference strain. The predicted auranofin target, thioredoxin reductase, was not mutated in any of our resistant lines. Subsequent whole genomic sequencing analysis (WGS) did not reveal a consensus resistance locus, although many have point mutations in genes encoding redox-relevant proteins such as superoxide dismutase (TgSOD2) and ribonucleotide reductase. We investigated the SOD2 L201P mutation and found that it was not sufficient to confer resistance when introduced into wild-type parasites. Resistant clones accumulated less ROS than their wild type counterparts. Our results demonstrate that resistance to auranofin in T. gondii enhances its ability to abate oxidative stress through diverse mechanisms. This evidence supports a hypothesized mechanism of auranofin anti-parasitic activity as disruption of redox homeostasis.

microbiology↗