Predicting statistics of gene translocation events: role of chromatin compaction and double-strand DNA break
How the 3D organization of chromatin affects gene translocation is an open question. By Monte Carlo simulation of translocation events in folded chromatin, we examine how the probability of translocation (Tij) is influenced by chromatin compaction and the occurrence of double-stranded DNA breaks (DSBs). Our results show that, beyond the two-segment contact probability, Tij depends on the overall compaction of the polymer and has a non-trivial contribution from multi-bead contacts and the probability of DSBs. We propose an empirical formula and provide analytical arguments for simple cases.
biophysics↗