Biology subjects

Isakovic, A. F.

Publications and source records attributed to Isakovic, A. F..

2 recordsLinked to original sources

bioRxiv2022

Phase Heterogeneity in Cholesterol-containing Ternary Phospholipid Lamellar Phases

Pseudo-ternary mixtures of lamellar phase phospholipids (DPPC and brain sphingomyelin with cholesterol) were studied below Tm, while comparing the influence of cholesterol content, temperature and the presence of small quantities of Vitamin D binding protein (DBP) or Vitamin D receptor (VDR). The measurements, conducted by X-ray diffraction (XRD) and nuclear magnetic resonance (NMR), cover a range of cholesterol concentrations (20% mol. wt to 40% mol. wt.) and physiologically relevant temperature range (294 K - 314 K). In addition to rich intra-phase behaviour, data and modelling are used to approximate the lipids headgroup location variations under the above-mentioned experimental conditions.

biophysics↗

bioRxiv2021

Electrodeformation of White Blood Cells Enriched with Gold Nanoparticles

The elasticity of white blood cells (WBCs) provides valuable insight into the condition of the cells themselves, the presence of some diseases, as well as immune system activity. In this work, we describe a novel process of refined control of WBCs elasticity through a combined use of gold nanoparticles (AuNPs) and the microelectrode array device. The capture and controlled deformation of gold nanoparticles enriched white blood cells in vitro are demonstrated and quantified. Gold nanoparticles enhance the effect of electrically induced deformation and make the DEP related processes more controllable.

biophysics↗