bioRxiv Science⌕ Search

Biology subjects

Hong, S. N.

Publications and source records attributed to Hong, S. N..

3 recordsLinked to original sources

IFN-gamma-Induced Intestinal Epithelial Cell-Type-Specific Programmed Cell Death: PANoptosis and Its Modulation in Crohn's Disease

Crohns disease (CD) is a chronic inflammatory bowel disease (IBD) and is considered a Th1-mediated disease, supported by the over-expression of interferon-gamma (IFN-{gamma}) in the intestinal lamina propria. To evaluate the response of intestinal epithelial cells (IECs) to IFN-{gamma}, we established small intestinal organoids (enteroids) derived from non-IBD controls and CD patients. IFN-{gamma} stimulated programmed cell death (PCD) of IECs in both control and CD enteroids in a dose-dependent manner. Pyroptosis, apoptosis and necroptosis were activated in enteroids, suggesting that PANoptosis was the main process of IFN-{gamma}-induced PCD in IECs. The response to IFN-{gamma} depends on the cell type of the IECs. IFN-{gamma} induced depletion of enterocytes with upregulation of PANoptosis-associated genes, while leading to expansion of goblet cells without significant change in PANoptosis-associated gene expression. Individual PCD inhibitors were insufficient to block IFN-{gamma}-induced cytotoxicity, whereas the selective JAK1 inhibitor (upadacitinib) effectively blocked IFN-{gamma}-induced cytotoxicity and PANoptosis. Furthermore, PANoptosis was significantly activated in surgically resected tissues and in publicly available single-cell RNA-sequencing datasets of intestinal tissues from patients with CD. In conclusion, IFN-{gamma} induces PANoptosis in enterocytes, which can be treated with a selective JAK1 inhibitor in patients with CD.

cell biology↗

Epithelial Regeneration Ability of Crohn's Disease Assessed Using Patient-Derived Intestinal Organoids

BackgroundThe intrinsic limitation of cell lines and animal models limits our understanding of epithelial regeneration capability in Crohns disease (CD). Therefore, we aimed to study epithelial regeneration ability in CD using an intestinal organoid model. Further, since tumor necrosis factor alpha (TNF) is a major proinflammatory effector during CD pathogenesis, we also investigated TNF-induced alteration of regeneration ability in CD patient-derived intestinal organoids. MethodsHuman intestinal organoids were constructed in a three-dimensional intestinal crypt culture of enteroscopic biopsy samples from control subjects and patients with CD. The epithelial regeneration ability of intestinal organoids was assessed using organoid reconstitution, 3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide (MTT), 5-ethynyl-2'-deoxyuridine (EdU), and wound healing assays. ResultsEx vivo cultures of ileal crypt cells revealed that organoid formation rate of CD patients were reduced compared with that of control subjects (p <.001). CD patient-derived organoids sub-cultured for more than 6 passages showed stable organoid reconstitution and identical morphological features. The organoid constitution and MTT assay revealed that the viability of TNF-treated CD patient-derived organoids were significantly lower than that of TNF-treated control organoids (p <.05 for each). The number of EdU+ proliferative cells was significantly lower in TNF-treated CD patient-derived organoids than in TNF-treated control organoids (p <.05). The wound-healing ability of TNF-treated CD patient-derived organoids was significantly lower than that of TNF-treated control organoids (p <.001). ConclusionsThe clinical trials are disabled to settle this issue, our results indicated that the epithelial regenerative ability is impaired in patients with CD, especially in TNF-enriched condition.

cell biology↗

Identification of a polymorphism in the N gene of SARS-CoV-2 that adversely impacts detection by a widely-used RT-PCR assay

We identify a mutation in the N gene of SARS-CoV-2 that adversely affects annealing of a commonly used RT-PCR primer; epidemiologic evidence suggests the virus retains pathogenicity and competence for spread. This reinforces the importance of using multiple targets, preferably in at least 2 genes, for robust SARS-CoV-2 detection. Article Summary LineA SARS-CoV-2 variant that occurs worldwide and has spread in California significantly affects diagnostic sensitivity of an N gene assay, highlighting the need to employ multiple viral targets for detection.

molecular biology↗