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Hasler, M.

Publications and source records attributed to Hasler, M..

3 recordsLinked to original sources

High-resolution disease phenotyping reveals distinct resistance strategies of wild tomato crop wild relatives against Sclerotinia sclerotiorum

Besides the well-understood qualitative disease resistance, plants possess a more complex quantitative form of resistance: quantitative disease resistance (QDR). QDR is commonly defined as a partial but more durable form of resistance and, therefore, might display a valuable target for resistance breeding. The characterization of QDR phenotypes, especially of wild crop relatives, displays a major bottleneck in deciphering QDRs genomic and regulatory background. Moreover, the relationship between QDR parameters, such as infection frequency, lag phase duration, and lesion growth rate, remains elusive. High hurdles for applying modern phenotyping technology, such as the low availability of phenotyping facilities or complex data analysis, further dampen progress in understanding QDR. Here, we applied a low-cost phenotyping system to measure lesion growth dynamics of wild tomato species (e.g., S. pennellii or S. pimpinellifolium). We provide insight into QDR diversity of wild populations and derive specific QDR strategies and their crosstalk. We show how temporally continuous observations are required to dissect end-point severity into functional resistance strategies. The results of our study show how QDR can be maintained by facilitating different defense strategies during host-parasite interaction and that the capacity of the QDR toolbox highly depends on the hosts genetic context. We anticipate that the present findings display a valuable resource for more targeted functional characterization of the processes involved in QDR. Moreover, we show how modest phenotyping technology can be leveraged to help answer highly relevant biological questions.

plant biology↗

Single-Molecule FRET at 10 MHz Count Rates

A bottleneck in many studies utilizing single-molecule Forster Resonance Energy Transfer (smFRET) is the attainable photon count rate as it determines the temporal resolution of the experiment. As many biologically relevant processes occur on timescales that are hardly accessible with currently achievable photon count rates, there has been considerable effort to find strategies to increase the stability and brightness of fluorescent dyes. Here, we use DNA nanoantennas to drastically increase the achievable photon count rates and to observe fast biomolecular dynamics in the small volume between two plasmonic nanoparticles. As a proof of concept, we observe the coupled folding and binding of two intrinsically disordered proteins which form transient encounter complexes with lifetimes on the order of 100 s. To test the limits of our approach, we also investigated the hybridization of a short single-stranded DNA to its complementary counterpart, revealing a transition path time of 17 s at photon count rates of around 10 MHz, which is an order-of-magnitude improvement when compared to the state of the art. Concomitantly, the photostability was increased, enabling many seconds long megahertz fluorescence time traces. Due to the modular nature of the DNA origami method, this platform can be adapted to a broad range of biomolecules, providing a promising approach to study previously unobservable ultrafast biophysical processes.

biophysics↗

Pseudo-data generation allows the statistical re-evaluation of toxicological bioassays based on summary statistics

Sometimes a re-analysis of toxicological data is useful. However, this usually requires the availability of the original data and in many cases only summary data are available in the publications. Here the generation of pseudo-data under certain assumptions using extension packages in the open-source project R on statistical computing is shown. Several case studies are used to illustrate the applicability in regulatory toxicology.

pharmacology and toxicology↗