Biology subjects

Hai, A.

Publications and source records attributed to Hai, A..

3 recordsLinked to original sources

bioRxiv2023

Wireless in vivo Recording of Cortical Activity by an Ion-Sensitive Field Effect Transistor

Wireless brain technologies are empowering basic neuroscience and clinical neurology by offering new platforms that minimize invasiveness and refine possibilities during electrophysiological recording and stimulation. Despite their advantages, most systems require on-board power supply and sizeable transmission circuitry, enforcing a lower bound for miniaturization. Designing new minimalistic architectures that can efficiently sense neurophysiological events will open the door to standalone microscale sensors and minimally invasive delivery of multiple sensors. Here we present a circuit for sensing ionic fluctuations in the brain by an ion-sensitive field effect transistor that detunes a single radiofrequency resonator in parallel. We establish sensitivity of the sensor by electromagnetic analysis and quantify response to ionic fluctuations in vitro. We validate this new architecture in vivo during hindpaw stimulation in rodents and verify correlation with local field potential recordings. This new approach can be implemented as an integrated circuit for wireless in situ recording of brain electrophysiology.

neuroscience↗

bioRxiv2022

Inference of Presynaptic Connectivity from Temporally Blurry Spike Trains by Supervised Learning

Reconstruction of neural network connectivity is a central focus of neuroscience. The ability to use neuronal connection information to predict activity at single unit resolution and decipher its effect on whole systems can provide critical information about behavior and cognitive processing. Neuronal sensing modalities come in varying forms, but there is yet to exist a modality that can deliver readouts that sufficiently address the spatiotemporal constraints of biological nervous systems. This necessitates supplementary approaches that rely on mathematical models to mitigate physical limitations and decode network features. Here, we introduce a simple proof-of-concept model that addresses temporal constraints by reconstructing presynaptic connections from temporally blurry data. We use a variation of the perceptron algorithm to process firing rate information at multiple time constraints for a heterogenous feed-forward network of excitatory, inhibitory, and unconnected presynaptic units. We evaluate the performance of the algorithm under these conditions and determine the optimal learning rate, firing rate, and the ability to reconstruct single unit spikes for a given degree of temporal blur. We then test our method on a physiologically relevant configuration by sampling network subpopulations of leaky integrate-and-fire neuronal models displaying bursting firing patterns and find comparable learning rates for optimized reconstruction of network connectivity. Our method provides a recipe for reverse engineering neural networks based on limited data quality that can be extended to more complicated readouts and connectivity distributions relevant to multiple brain circuits.

neuroscience↗

bioRxiv2021

Stimulation-mediated reverse engineering of silent neural networks

Reconstructing connectivity of neuronal networks from single cell activity is essential to understanding brain function, but the challenge of deciphering connections from populations of silent neurons has been largely unmet. We demonstrate a protocol for deriving connectivity of realistic silent neuronal networks using stimulation combined with a supervised learning algorithm, that enables inferring connection weights with high fidelity and predicting spike trains at the single-spike and single-cell level with high accuracy. These testable predictions about the number and protocol of the required stimulations is expected to enhance future efforts for deriving neuronal connectivity and drive new experiments to better understand brain function.

neuroscience↗