mRNArchitect: optimized design of mRNA sequences
Sequence design is the critical first step in developing a new mRNA medicine. The mRNA primary sequence is assembled from multiple elements, including the coding sequence of the target protein or gene, flanking untranslated regions that impact expression, and the poly(A) tail, which improves stability. The mRNA primary sequence can be optimized to improve the performance of an mRNA medicine, including increasing translation and stability, and reducing reactogenicity. Here, we introduce mRNArchitect, a software toolkit to assist in the design of mRNA sequences. mRNArchitect can rapidly assemble and optimize mRNA sequences based on user requirements, including GC content, secondary structure, codon optimization, and uridine depletion. The sequences generated by mRNArchitect can also be readily synthesized and have been experimentally validated across a variety of applications. We present mRNArchitect as a software toolkit to help scientists design new mRNA medicines. Availability and implementationmRNArchitect is available via a web portal at www.basefacility.org.au/software/ and Github at https://github.com/BaseUQ/mRNArchitect. mRNArchitect is open-source and freely available under MIT License.