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Gonzalez-Garcia, J.

Publications and source records attributed to Gonzalez-Garcia, J..

2 recordsLinked to original sources

Monitoring jumping ability and subjective recovery in women's volleyball: acute effects of match play on countermovement jump and well-being

PurposeJumping ability is a crucial element for success in volleyball. Monitoring it helps optimize performance and prevent injuries. The countermovement jump (CMJ) is a reliable, non-invasive, and quick tool to periodically assess neuromuscular fatigue and performance, which, in combination with subjective measures, can offer a comprehensive view of fatigue/recovery status. The study aimed to: a) analyze changes in CMJ after a womens volleyball match based on playing time and b) evaluate the temporal recovery of subjective variables TQR (perceived recovery) and HI (well-being). It was hypothesized that players would recover baseline values of CMJ and subjective variables 48 hours post-match. MethodsA descriptive, longitudinal, and repeated-measures design was proposed. Twelve volleyball players were evaluated for changes in CMJ before and after a match, and at 24-, 48-, and 72-hours post-match. Outcome variables (jump height and RSImod), kinetic variables (concentric impulse), and jump strategy variables (contraction time and time to peak power) were analyzed. TQR and HI scales assessed perceived recovery and well-being. ResultsSignificant changes were observed in jump height, concentric impulse, and RSImod between pre-match and 48 hours post-match in players with less playing time. Subjective variables TQR and HI did not show significant differences during the study period. ConclusionCompetition did not significantly affect CMJ variables, suggesting that volleyball does not reduce jumping ability immediately after competing, which has direct implications for microcycle structure. The study highlights the importance of continuous monitoring of performance and recovery in volleyball.

physiology↗

Visualising G-quadruplex DNA dynamics in live cells by fluorescence lifetime imaging microscopy

Guanine rich regions of oligonucleotides fold into quadruple-stranded structures called G-quadruplexes (G4). Increasing evidence suggests that these G4 structures form in vivo and play a crucial role in cellular processes. However, their direct observation in live cells remains a challenge. Here we demonstrate that a fluorescent probe (DAOTA-M2) in conjunction with Fluorescence Lifetime Imaging Microscopy (FLIM) can identify G4 within nuclei of live and fixed cells. We present a new FLIM-based cellular assay to study the interaction of non-fluorescent small molecules with G4 and apply it to a wide range of drug candidates. We also demonstrate that DAOTA-M2 can be used to study G4 stability in live cells. Reduction of FancJ and RTEL1 expression in mammalian cells increases the DAOTA-M2 lifetime and therefore suggests an increased number of G4 in these cells, implying that FancJ and RTEL1 play a role in resolving G4 structures in cellulo.

biophysics↗