Biology subjects

Glick, L.

Publications and source records attributed to Glick, L..

3 recordsLinked to original sources

bioRxiv2025

Evolutionary history, ecological divergence, and introgression in the Oncocyclus irises species complex in the Southern Levant

Speciation is a dynamic process shaped by the interaction of gene flow, geographic isolation, and ecological divergence. The Oncocyclus irises of the Southern Levant represent a young radiation of narrowly endemic species considered to be in the course of speciation. In this study, we used RAD-sequencing and single nucleotide polymorphism analysis across nine described species to investigate patterns of genomic divergence, gene flow, and local adaptation. Phylogenomic analyses revealed a mix of well-supported clades for some species, previously defined by their morphology and distribution, and non-monophyletic lineages, with several species exhibiting shallow divergence and shared genetic ancestry. We found evidence for gene flow and historical introgression between Iris petrana, I. atrofusca and I. mariae, while other cases of non-monophyly appear driven by incomplete lineage sorting. Both geographic distance (IBD) and environmental factors (IBE), mainly altitude, temperature, and aridity, were significantly associated with genetic structure, suggesting that local adaptation contributed to divergence following range expansion. Based on our findings we propose that the divergence of the Oncocyclus iris species in the Southern Levant supports a stepping-stone dispersion model, in which north-to-south dispersal was followed by local adaptation, and introgression in secondary contact zones. Overall, these findings highlight the complexity of speciation and the need for integrative approaches to study the interplay between historical divergence, contemporary gene flow, and environmental differentiation in shaping genomic patterns.

evolutionary biology↗

bioRxiv2024

Whole-Genome Sequencing of the Wild Barley Diversity Collection: A Resource for Identifying and Exploiting Genetic Variation for Cultivated Barley Improvement

To exploit allelic variation in Hordeum vulgare subsp. spontaneum, the Wild Barley Diversity Collection was evaluated for several agronomic traits and subjected to paired-end Illumina sequencing at [~]9X depth, generating 109.5 million single nucleotide polymorphisms after alignment to the Morex V3 assembly. A genome-wide association study of lemma color identified one marker-trait association (MTA) on chromosome 1HL close to HvBlp, the cloned gene controlling black lemma. Four MTAs were identified for stem rust resistance: one co-locating to the complex RMRL1-RMRL2 locus on 5HL, and three novel loci on 1HS, 1HL, and 5HL. Six MTAs for days to heading (DTH) on vernalized plants were identified on all chromosomes except 1H and 6H. Two MTAs for DTH on non-vernalized plants were identified on chromosomes 1HL and 2HS. All MTAs for DTH were novel. The whole genome sequence data described herein will facilitate the identification and utilization of new alleles for barley improvement.

genomics↗

bioRxiv2022

The evolutionary dynamics that retain long neutral genomic sequences in face of indel deletion bias: a model and its application to human introns

Insertions and deletions (indels) of short DNA segments are common evolutionary events. Numerous studies showed that deletions occur more often than insertions in both prokaryotes and eukaryotes. It raises the question why neutral sequences are not eradicated from the genome. We suggest that this is due to a phenomenon we term border-induced selection. Accordingly, a neutral sequence is bordered between conserved regions. Deletions occurring near the borders occasionally protrude to the conserved region and are thereby subject to strong purifying selection. Thus, for short neutral sequences, an insertion bias is expected. Here, we develop a set of increasingly complex models of indel-dynamics that incorporate border-induced selection. Furthermore, we show that short conserved sequences within the neutrally evolving sequence help explain: (1) the presence of very long sequences; (2) the high variance of sequence lengths; (3) the possible emergence of multimodality in sequence length distributions. Finally, we fitted our models to the human intron length distribution, as introns are thought to be mostly neutral and bordered by conserved exons. We show that when accounting for the occurrence of short conserved sequences within introns, we reproduce the main features, including the presence of long introns and the multimodality of intron distribution.

bioinformatics↗