Glenna, S. | bioRxiv Science

bioRxiv2025

Longitudinal analysis of the hand microbiome in response to chlorine-based antiseptic use during a military field exercise

Hand hygiene is essential for infection control, yet the impact of frequent antiseptic use on the skin microbiome, which is crucial for skin barrier function and pathogen exclusion, remains underexplored, especially in field conditions. In high-risk military settings, there is a need for safe, multipurpose antiseptics that avoid the drawbacks of alcohol-based options, including skin irritation, flammability, and unsuitability for wound application. We conducted a longitudinal study during the Norwegian-led military exercise Cold Response 2022 to assess the effects of repeated use of stabilized hypochlorous acid, a non-alcoholic antiseptic, on the hand microbiome of soldiers in a field environment. Participants used either the chlorine-based antiseptic (n = 20) or standard hygiene practices, mainly alcohol-based sanitizers (n = 19), for 10 days. Skin swabs from hands and untreated forearms were collected at baseline, post-intervention, and three weeks after discontinuation, and analyzed using 16S rRNA sequencing. Overall, our data revealed that field exposure accounted for more variation in bacterial composition than antiseptic use. When comparing alpha and beta diversity trajectories between the two antiseptic groups, there was minimal divergence during active field use, but a significant post-treatment increase in alpha diversity associated with the use of chlorine-based antiseptic. In contrast, untreated forearms showed no such group-level differences. These findings suggest that antiseptic-driven microbiome shifts are most apparent during recovery, with non-alcoholic formulations supporting faster recolonization after treatment. Stabilized hypochlorous acid represents a promising alternative to standard alcohol-based antiseptics for military personnel. IMPORTANCEEffective hand hygiene reduces pathogen transmission, but its influence on the skin microbiome has rarely been studied. Our research emphasizes the importance of longitudinal assessments by identifying greater antiseptic-driven differences occurring after cessation of treatment than during repeated use. Service members frequently use antiseptics in challenging environments, highlighting the operational need for a non-alcoholic, multipurpose product that can be safely used on both intact skin and wounds. Our findings suggest that a chlorine-based non-alcoholic antiseptic permits faster recovery of bacterial diversity after perturbation, informing product selection and hygiene policies in defense and other high-risk settings.

microbiology↗

bioRxiv2024

Skin bacterial community dynamics of hands and forearms before and after military field exercise

The human skin microbiome is crucial for skin health and immunity, especially in the context of extreme conditions faced by military personnel. Soldiers encounter unique stressors and hygienic challenges that can impact the microbial composition of their skin during field exercises and in regular occupational settings. In this study, we aimed to investigate the impact of a military field exercise on the diversity and composition of the skin bacterial microbiota using 16S rRNA sequencing. We conducted a longitudinal study during the NATO exercise Cold Response 2022, involving Norwegian soldiers (n = 19) engaged in outdoor training operations. Skin swabs were taken from soldiers hands and forearms directly before and after the 10-day winter field exercise, and following a 3-week post-exercise leave. Our results reveal hand and forearm-specific shifts in bacterial populations associated with the exercise, likely influenced by environmental exposure, reduced hygiene, and heightened social contact. Alpha diversity increased on forearms while remaining stable on hands, which appeared more resilient to perturbations. Both sites exhibited temporal changes in composition, with soil- and water-associated bacteria enriched post-exercise; most being transient on hands but more sustained on forearms. The soldiers microbiomes became more similar during the exercise, followed by divergence in the leave period. Neither skin site returned to original composition at follow-up, indicating that field exercises may have lasting effects on the microbiome. Our findings highlight the impact of outdoor exposure on microbial communities and suggest that resilience and stability differ between skin sites. ImportanceOptimizing soldier health and resilience is critical for maintaining military readiness and operational effectiveness. The skin, as the bodys first line of defense, is subjected to numerous challenges in military environments. Unique environmental and hygiene challenges can disrupt the skin microbiome and increase susceptibility to skin and soft tissue infections. This longitudinal research provides valuable insights into the effects of military service on the bacterial dynamics of the skin microbiome, but can also inform hygiene management and disease prevention in comparable situations.

microbiology↗