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Biology subjects

Gil, P. I.

Publications and source records attributed to Gil, P. I..

3 recordsLinked to original sources

Promising Inhibitor of Flavivirus: Evaluating the Antiviral Activity of Nordihydroguaiaretic Acid against West Nile Virus Replication

Mosquito-borne Arboviruses, including Alphaviruses and Flaviviruses, are responsible for millions of human infections worldwide. The absence of specific antivirals for arbovirus infections underscores the urgent need for novel research to develop effective treatments. Natural products have emerged as promising candidates. Nordihydroguaiaretic acid (NDGA), a natural lignan predominantly found in Larrea spp., has shown antiviral activity against several viruses, including Dengue. This study aimed to investigate the antiviral potential of NDGA against West Nile virus (WNV), assessing its mechanism of action and cellular interactions. Using plaque-forming unit reduction and immunofluorescence assays in LLC-MK2 cells, we determine the reduction in viability of WNV viral particles at different times post-infection. The potential involvement of the sterol regulatory element-binding proteins (SREBP) and 5-lipoxygenase pathways in WNV infection was evaluated. Additionally, we analyzed the cytoskeleton integrity in treated cells. NDGA, had the greatest inhibitory effect on WNV replication when added 1 and 2 h post-viral internalization (PI), losing activity when added after 3 h PI, thus suggesting it targets early viral infection events. The SREBP pathway seems to be involved in the inhibition of WNV by NDGA, likely due to its lipogenic activity. Furthermore, the antiviral activity of NDGA could be related to its ability to modify the cytoskeleton of LLC-MK2 cells. These findings highlight NDGA potential as a therapeutic candidate against WNV and other flaviviruses. Plant metabolites are among the leading therapeutic resources globally. Further research is necessary to comprehensively assess the therapeutic promise of NDGA. ImportanceMosquito-borne arboviruses pose significant global health threats due to their widespread distribution and the absence of specific antiviral treatments. The emergence of virulent strains and recurrent outbreaks underscore the urgent need for novel antiviral agents. Nordihydroguaiaretic acid, a natural phenolic compound, has shown promising antiviral activity against flaviviruses such as West Nile virus. Understanding nordihydroguaiaretic acids mechanism of action not only reveals potential therapeutic strategies but also highlights the broader importance of plant-derived compounds in combating viral infections. Our research focuses on further elucidating nordihydroguaiaretic acids antiviral effects against West Nile virus, aiming to contribute to the development of effective therapies. Continued investigation of nordihydroguaiaretic acids therapeutic potential is essential for advancing antiviral drug discovery and addressing the persistent challenges posed by mosquito-borne diseases worldwide.

microbiology↗

Actin cytoskeleton dynamics affect replication of Human Metapneumovirus

Human Metapneumovirus (hMPV) is responsible for viral respiratory infection with clinical and epidemiological relevance in pediatric, immunocompromised, and elderly populations. Little is known about hMPV in vitro replication processes and their relationship with cellular structures such as the cytoskeleton. Our goal was to evaluate the role of the actin cytoskeleton in hMPV replication at different stages of viral growth. hMPV was isolated in Vero cells from a clinical sample and identified as A2 genotype. The cytopathic effect was detected by the appearance of cell rounding and refractory cell clusters. The growth curve showed that viral replication maximum level was between 48 and 72 hpi. The highest percentage of infected cells and intracellular hMPV-protein were detected at the early stages of the replication cycle. Disruption of actin microfilaments with Cytochalasin D during the early events provoked an increase in both intracellular and extracellular viruses. We demonstrate that the early phase of the hMPV curve is crucial for viral replication; and the disruption of microfilaments during this time increments both viral protein expression and release of viruses to the extracellular space. This study contributes to elucidate wild-type hMPV growth kinetics, providing new insights on the actin cytoskeleton role in viral replication mechanisms.

microbiology↗

Functional entry of Members of Venezuelan Equine Encephalitis Complex into host cells is dependent on clathrin-mediated endocytosis, in a pH-Dependent Manner

Pixuna virus (PIXV) and Rio Negro virus (RNV) are mosquito-borne alphaviruses belonging to the Venezuelan Equine Encephalitis (VEE) complex, which includes pathogenic epizootic and enzootic subtypes responsible for life-threatening diseases in equines. Considering that the first steps in viral infection are crucial for the efficient production of new progeny, the aim of this study was to elucidate the early events of the replication cycle of these two viruses. To this end, we used chemical inhibitors and the expression of dominant-negative constructs to study the dependence of clathrin and endosomal pH on PIXV and RNV internalization mechanisms. We demonstrated that both viruses are internalized primarily via clathrin-mediated endocytosis, where the low pH in endosomes is crucial for viral replication. Contributing knowledge regarding the entry route of VEE complex members is important to understand the pathogenesis of these viruses and also to develop new antiviral strategies.

microbiology↗