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Gibbs, J. L.

Publications and source records attributed to Gibbs, J. L..

4 recordsLinked to original sources

Hypomineralized Enamel Alters Trigeminal Sensory Afferent Architecture, Transcriptome, and Dental Injury Responses

At barrier tissues such as the skin and gut, sensory neurons interface with environmental stimuli and coordinate with neighboring epithelial and immune cells to detect tissue perturbations. In teeth, however, the sensory dentin-pulp complex is insulated from the oral environment by highly mineralized enamel. Although dentin-pulp responses to severe injury have been studied in models with direct pulp exposure, it remains unclear whether enamel barrier dysfunction alone alters pulpal and neuronal homeostasis. Using a kallikrein-related peptidase 4 knockout (KLK4 KO) mouse model of enamel hypomineralization, we demonstrated that defective enamel induced structural, molecular, and transcriptional responses in the dental pulp and in the trigeminal system innervating teeth, despite the absence of direct pulp exposure to the oral cavity. Hypomineralized molars exhibited increased reactionary dentin formation accompanied by retraction of sensory afferents from the dentin-pulp junction. Consistent with these structural findings, trigeminal ganglia of KLK4 KO mice displayed upregulation of genes associated with cytoskeletal remodeling and stimulus response pathways. Despite increased bacterial burden and biofilm accumulation on the enamel surface, enamel hypomineralization did not induce substantial innate immune responses in the pulp. In addition, following severe dental pulp injury, teeth with hypomineralized enamel exhibited reduced sensory afferent loss and tissue damage. Together, these findings reveal that enamel integrity functions as a critical regulator of dentin-pulp homeostasis and that barrier dysfunction alone can precondition tissue responses to subsequent injury.

neuroscience↗

Dynamics of Innate Immune Response Due to Bacteria-Induced Pulpitis

IntroductionPulpitis is associated with dental carries and can lead to irreversible pulp damage. As bacteria penetrate deeper into dentin and pulp tissue, a pulpal innate immune response is initiated. However, an understanding of the types of immune cells in the pulp, how this relates to bacterial infiltration, and the dynamics of the immune response during pulpitis is limited. As conserving the vitality of the pulp tissue through conservative therapies becomes an important part of dental practice, there is a greater need to understand the kinetics and composition of the immune response during pulpitis. MethodsDental pulp exposure in molars of mice was used as an animal model of pulpitis. To investigate the kinetics of immune response, pulp tissue was collected from permanent molars at different time points after injury (baseline, day 1, and day 7). Flow cytometry analysis of CD45+ leukoctyes including macrophages, T cells, neutrophils and monocytes was performed. 16S in situ hybridization captured bacterial invasion of the pulp, and immunohistochemistry for F4/80 investigated spatial and morphological changes of macrophages during pulpitis. Data were analyzed using two-way ANOVA with Tukeys multiple comparisons. ResultsBacteria mostly remained close to the injury site, with some expansion towards non-injured pulp horns. We found that F4/80+ macrophages were the main immune cell population in healthy pulp. Upon injury, CD11b+Ly6Ghigh neutrophils and CD11b+Ly6GintLy6Cint monocytes constituted 70-90% of all immune populations up to 7 days after injury. Even though there was a slight increase in T cells at day 7, myeloid cells remained the main drivers of the immune response. ConclusionsAs bacteria proliferate within the pulp chamber, innate immune cells including macrophages, neutrophils and monocytes predominate as the major immune populations, with minimal signs of transitioning to an adaptive immune response.

immunology↗

Neuronal-immune Axis Alters Pain and Sensory Afferent Damage During Dental Pulp Injury

Dental pulp tissue is densely innervated by afferent fibers of the trigeminal ganglion. When bacteria cause dental decay near the pulpal tissue, a strong neuronal and immune response occur, creating pulpitis, which is associated with severe pain and pulp tissue damage. Neuro-immune interactions have the potential to modulate both the pain and pathological outcome of pulpitis. We first investigated the role of the neuropeptide calcitonin-gene related peptide (CGRP), released from peptidergic sensory afferents, in dental pain and immune responses by using calca knock out (calca-/-) and wild type (calca+/+) mice, in a model of pulpitis by creating a mechanical exposure of the dental pulp horn. While CGRP did not contribute to facial mechanical hypersensitivity, at an early time point, it did contribute to spontaneous pain-like behavior. We also found that CGRP contributed to recruitment of neutrophils and monocytes, while not clearly affecting the progression of pulpal pathology histologically. When we depleted neutrophils and monocytes, we found that there was more sensory afferent loss, tissue damage and deeper spread of bacteria into the pulp tissue, while there was a reduction in facial mechanical hypersensitivity compared to control animals at a later time point. Overall, we showed that there is a crosstalk between peptidergic neurons and neutrophils in the pulp, modulating the pain and inflammatory outcomes of the disease.

neuroscience↗

Evaluation of TRPM8 and TRPA1 participation in dental pulp sensitivity to cold stimulation

Sensory neurons innervating the dental pulp have unique morphological and functional characteristics compared to neurons innervating other tissues. Stimulation of dental pulp afferents whatever the modality or intensity of the stimulus, even light mechanical stimulation that would not activate nociceptors in other tissues, produces an intense pain. These specific sensory characteristics could involve receptors of the Transient Receptor Potential channels (TRP) family. In this study, we evaluated 1) the expression of TRPA1 and TRPM8 receptors in trigeminal ganglion neurons innervating the dental pulp compared to sensory neurons innervating the oral mucosa or the skin of the face, and 2) the involvement of these receptors in dental pulp sensitivity to cold stimulation. We showed a similar expression of TRPM8 and CGRP in sensory neurons innervating the dental pulp, the skin or the oral mucosa. However, the proportion of neurons expressing TRPA1 was higher in the neuronal population innervating the mucosa (43%) than in the neuronal population innervating the dental pulp (19%) or the skin (24%). Moreover, neurons innervating the dental pulp had a higher proportion of large neurons (24%) compared to neurons innervating the skin (8%) or the mucosa (10%). The evaluation of the relation between TRPM8, TRPA1 and cold stimulation in trigeminal ganglion neuron culture, showed that a significant proportion of neurons innervating the skin (10%) or the mucosa (37%) were sensitive to cold stimulation but insensitive to TRPM8 and TRPA1 activation, indicating that perception of stimuli would be in part independent to TRPA1 and TRPM8 receptors. Similarly, the application of a cold stimulation on the tooth induced an overexpression of cFos in the trigeminal nucleus that was not prevented by administration of a TRPA1 antagonist or the genetic deletion of TRPM8. However, the pretreatment with the local anesthetic carbocaine abolish the cold-induced cFos overexpression. In conclusion, the unique sensory characteristics of the dental pulp would be independent to TRPA1 and TRPM8 expression and functionality.

neuroscience↗