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Gaudon, V.

Publications and source records attributed to Gaudon, V..

2 recordsLinked to original sources

A coordinated haptic mechanism ensures efficient DNA sampling by the 8-oxoguanine glycosylase OGG1.

7,8-Dihydro-8-oxoguanine (8-oxoG) is the most frequent base modification occurring upon oxidative stress. This highly mutagenic lesion is specifically recognized and excised by the DNA glycosylase OGG1 when paired with cytosine, initiating the base excision repair pathway. Since 8-oxoG neither significantly impacts the structure of the double helix nor blocks transcription and replication processes, its detection requires a careful inspection of each base pair by OGG1. By monitoring this lesion search process both in vitro and in living cells, we demonstrate that it involves a tight coordination between several conserved amino acids encircling the DNA helix. More specifically, we show that the N149-151 motif, on the target strand, as well as residues R154 and R204, on the opposite strand, both regulate OGG1 engagement on the DNA to ensure fast Y203-mediated base unstacking, a prerequisite for efficient 8-oxoG detection. These findings highlight the early mechanisms that enable OGG1 to maintain rapid sampling kinetics while preserving high specificity for 8-oxoG in the context of the complex architecture displayed by the DNA within the cell nucleus.

genetics↗

Identification of key residues of the DNA glycosylase OGG1 controlling efficient DNA scanning and recruitment to oxidized bases in living cells

The DNA-glycosylase OGG1 oversees the detection and clearance of the 7,8-dihydro-8-oxoguanine (8-oxoG), which is the most frequent form of oxidized base in the genome. This lesion is deeply buried within the double-helix and its detection requires careful inspection of the bases by OGG1 via a mechanism that remains only partially understood. By analyzing OGG1 dynamics in the nucleus of living human cells, we demonstrate that the glycosylase constantly scans the DNA by rapidly alternating between diffusion within the nucleoplasm and short transits on the DNA. This scanning process, that we find to be tightly regulated by the conserved residue G245, is crucial for the rapid recruitment of OGG1 at oxidative lesions induced by laser micro-irradiation. Furthermore, we show that residues Y203, N149 and N150, while being all involved in early stages of 8-oxoG probing by OGG1 based on previous structural data, differentially regulate the scanning of the DNA and recruitment to oxidative lesions.

cell biology↗