Biology subjects

Galan-Arriola, C.

Publications and source records attributed to Galan-Arriola, C..

4 recordsLinked to original sources

bioRxiv2025

Towards whole-heart quantitative myocardial perfusion using a dual-sequence framework with multiband acceleration

Background2D Quantitative Myocardial Perfusion (Qperf) MRI is limited by its inability to provide complete myocardial coverage within a heartbeat interval. This study developed and evaluated dual saturation multiband-accelerated Qperf imaging to achieve near-complete left ventricular coverage in free-breathing at an adequate in-plane spatial resolution, using a semi-automated Myocardial Blood Flow (MBF) framework for quantification deployable directly on the scanner console. MethodsA dual-saturation single-band QPerf sequence was modified for multiband imaging, enabling the acquisition of 6 high-resolution myocardial slices plus Arterial Input Function (AIF) during free-breathing. The technique was evaluated in 16 sedated pigs (13 healthy and 3 with LAD occlusion) under rest conditions on a 3T MRI scanner. Additionally, two healthy pigs underwent stress imaging as well. Statistical comparisons were performed between multiband and single-band MBF values in corresponding AHA segments. ResultsQualitatively, multiband QPerf provided superior left ventricular coverage and comparable image quality to single-band Qperf MBF maps, potentially enabling a more comprehensive detection of perfusion defects at rest. Quantitatively, multiband QPerf yielded lower MBF values than single-band QPerf (p < 0.01). However, Bland-Altman analysis (mean difference: -0.17 ml/min/g; 95% CI: -1.12 to 0.79 ml/min/g) and Passing-Bablok regression (intercept: -0.01 ml/min/g; 95% CI: - 0.37 to 0.28 ml/min/g) indicated that such discrepancy remained within the expected confidence intervals. Furthermore, the Passing-Bablok slope (0.88; 95% CI: 0.73-1.06) confirmed that mb-QPerf maintained sensitivity comparable to sb-QPerf in detecting perfusion changes under rest conditions. Finally, there was an overall increase in MBF values during stress vs rest conditions (average MBF Ratio 1.67 {+/-} 0.31) when comparing healthy pigs. ConclusionMultiband-accelerated Qperf is feasible, providing improved left ventricular coverage, adequate in-plane resolution, and a semi-automated MBF quantification framework directly on the scanner console. Compared to its single-band counterpart, multiband QPerf demonstrated a more comprehensive visualization of perfusion defects and comparable sensitivity and accuracy in detecting perfusion changes at rest. Further research and clinical validation in patient populations are needed to confirm its utility in the diagnosis of coronary artery disease.

bioengineering↗

bioRxiv2025

Precon_all: A species-agnostic automated pipeline for non-human cortical surface reconstruction

Cortical surface reconstruction has changed how we study brain morphology and geometry. However, extending these methods to non-human species has been limited by the lack of standardized pipelines, anatomical templates, and variability in imaging protocols. To address these challenges, we present Precon_all, an open-source, species-agnostic pipeline that automates cortical surface reconstruction for non-human neuroimaging. It runs reliably across a wide range of anatomical structures and imaging conditions and has been successfully applied to datasets from primates, carnivores, and artiodactyls. Its modular framework mirrors human neuroimaging workflows, supports manual quality control, and produces outputs compatible with FreeSurfer and Connectome Workbench. In doing so, it substantially reduces technical barriers to non-human neuroimaging. As data-sharing initiatives continue to expand access to non-human imaging datasets, Precon_all provides a scalable and standardized solution that supports the broader adoption of surface-based methods for studying cortical evolution through comparative neuroscience.

neuroscience↗

bioRxiv2024

Protein aggregation capture assisted profiling of the thiol redox proteome

Oxidative damage is critical in various diseases, including cardiovascular and neurological conditions. Thiol redox reactions, acting as oxidative stress sensors, influence protein structure and function. Redox proteomics based on differential alkylation of reduced and oxidized Cys forms using mass spectrometry enables comprehensive analysis of thiol redox status in cells and tissues. We introduce PACREDOX, an innovative redox proteomics approach based on the Protein Aggregation Capture (PAC) protocol and we demonstrate its compatibility with library free data-independent acquisition (DIA). PACREDOX reduces preparation time and costs compared to traditional methods, such as FASILOX, while maintaining thiol and proteome coverage. To enable library-free DIA, we corrected in silico spectral libraries in DIA-NN using experimental retention time data from beta-methylthiol-modified peptides. PACREDOX with DIA quantified 4,000 protein groups and [~]45,000 modified peptides in myocardial tissue from a porcine model of atrial fibrillation, including over 8,000 cysteine-containing peptides, 30% of which were reversibly oxidized. Benchmarking PACREDOX and DIA against FASILOX in a myocardial infarction model reflects the potential and efficiency of this methodology to study oxidative damage. Overall, PACREDOX offers a high-throughput, cost-effective strategy for thiol redox proteome analysis, compatible with label-free quantitative workflows.

systems biology↗

bioRxiv2020

Remote Ischemic Preconditioning Ameliorates Anthracycline-induced Cardiotoxicity and Preserves Mitochondrial Integrity

AimsAnthracycline-induced cardiotoxicity (AIC) is a serious adverse effect in a significant proportion of cancer patients. A central mechanism of AIC is irreversible mitochondrial damage. Despite major efforts, there are currently no effective therapies able to prevent AIC. Methods and ResultsForty Large-White pigs were included. In Study 1, 20 pigs were randomized 1:1 to remote ischemic pre-conditioning (RIPC, 3 cycles of 5 min leg ischemia followed by 5 min reperfusion) or no pretreatment. RIPC was performed immediately before each of five intracoronary doxorubicin injections (0.45 mg/kg) given at weeks 0, 2, 4, 6, and 8. A group of 10 pigs with no exposure to doxorubicin served as healthy controls. Pigs underwent serial cardiac magnetic resonance (CMR) exams at baseline and at weeks 6, 8, 12, and 16. After 16-week CMR, pigs were sacrificed and tissue samples collected. In study 2, 10 new pigs received 3 doxorubicin injections (with/out preceding RIPC) and were sacrificed 2 weeks after the third dose. In Study 1, LVEF remained unchanged in doxorubicin-treated pigs until week 6 (time of the fourth doxorubicin injection). From there on, LVEF progressively declined, but LVEF depression was blunted animals receiving RIPC before doxorubicin (RIPC-Doxo), which had a significantly higher LVEF at week 16 than doxorubicin treated pigs that received no pretreatment (Untreated-Doxo) (41.5{+/-}9.1% vs 32.5{+/-}8.7%, p=0.04). Preserved LVEF was mainly due to conserved contractile function, as evidenced by smaller LVESV, and better regional contractile function. In Study 2, transmission electron microscopy (TEM) after 3 doxorubicin doses showed fragmented mitochondria with severe morphological abnormalities in RIPC+Doxo pigs, together with upregulation of fission proteins and autophagy markers on western blot. At the end of the 16-week Study 1 protocol, TEM revealed overt mitochondrial fragmentation with structural fragmentation in Untreated-Doxo pigs, whereas interstitial fibrosis was significantly less severe in the RIPC+Doxo pigs. ConclusionIn a translatable large animal model of AIC, RIPC applied immediately before each doxorubicin injection resulted in preserved cardiac contractility with significantly higher long-term LVEF and less cardiac fibrosis. RIPC prevented mitochondrial fragmentation and dysregulated autophagy from the early stages of AIC. RIPC is a promising intervention for testing in clinical trials in AIC.

molecular biology↗