Gagnon, G. A. | bioRxiv Science

bioRxiv2021

Effect of sodium silicate on drinking water biofilm development

Sodium silicates have been studied for sequestration of iron, coagulation, and corrosion control, but their impact on biofilm formation has not been documented in detail. This study investigated the impact of sodium silicate corrosion control on biomass accumulation in drinking water systems in comparison to orthophosphate, a common corrosion inhibitor. Biofilm growth was measured by determining ATP concentrations, and the bacterial community was characterized using 16S ribosomal RNA (rRNA) sequencing. A pilot-scale study with cast-iron pipe loops, annular reactors (ARs), and polycarbonate coupons demonstrated significantly lower biofilm ATP concentrations in the sodium silicate-treated AR than the orthophosphate-treated AR when the water temperature exceeded 20{degrees}C. However, an elevated sodium silicate dose (48 mg L-1 of SiO2) disturbed and dispersed the biofilm formed inside the AR, resulting in elevated effluent ATP concentrations. Two separate experiments confirmed that biomass accumulation was higher in the presence of orthophosphate at high water temperatures (20{degrees}C) only. No significant differences were identified in biofilm ATP concentrations at lower water temperatures (below 20{degrees}C). Differences in bacterial communities between the orthophosphate- and sodium silicate-treated systems were not statistically significant, even though orthophosphate promoted higher biofilm growth. However, the genera Halomonas and Mycobacterium--which include opportunistic pathogens--were present at greater relative abundances in the orthophosphate-treated system compared to the sodium silicate system. Graphical abstract O_FIG O_LINKSMALLFIG WIDTH=200 HEIGHT=111 SRC="FIGDIR/small/460503v1_ufig1.gif" ALT="Figure 1"> View larger version (31K): org.highwire.dtl.DTLVardef@14ac86corg.highwire.dtl.DTLVardef@ac500borg.highwire.dtl.DTLVardef@bb2980org.highwire.dtl.DTLVardef@1a1b5c0_HPS_FORMAT_FIGEXP M_FIG C_FIG Orthophosphate promotes more biofilm growth in comparison to sodium silicates at water temperatures above 20{degrees}C. Water impact statementSodium silicates have been used in drinking water treatment for decades, both as sequestrants and as corrosion inhibitors. However, their impact on biofilm formation is poorly understood, and this risks drinking water quality. This study aims to further clarify the effects of corrosion inhibitors on biofilm development, including inhibitors that are not phosphate-based.

microbiology↗

bioRxiv2020

Pandemic danger to the deep: the risk of marine mammals contracting SARS-CoV-2 from wastewater

We are in unprecedented times with the ongoing COVID-19 pandemic. The pandemic has impacted public health, the economy and our society on a global scale. In addition, the impacts of COVID-19 permeate into our environment and wildlife as well. Here, we discuss the essential role of wastewater treatment and management during these times. A consequence of poor wastewater management is the discharge of untreated wastewater carrying infectious SARS-CoV-2 into natural water systems that are home to marine mammals. Here, we predict the susceptibility of marine mammal species using a modelling approach. We identified that many species of whale, dolphin and seal, as well as otters, are predicted to be highly susceptible to infection by the SARS-CoV-2 virus. In addition, geo-mapping highlights how current wastewater management in Alaska may lead to susceptible marine mammal populations being exposed to the virus. Localities such as Cold Bay, Naknek, Dillingham and Palmer may require additional treatment of their wastewater to prevent virus spillover through sewage. Since over half of these susceptibility species are already at risk worldwide, the release of the virus via untreated wastewater could have devastating consequences for their already declining populations. For these reasons, we discuss approaches that can be taken by the public, policymakers and wastewater treatment facilities to reduce the risk of virus spillover in our natural water systems. Thus, we indicate the potential for reverse zoonotic transmission of COVID-19 and its impact on marine wildlife; impacts that can be mitigated with appropriate action to prevent further damage to these vulnerable populations.

ecology↗