Biology subjects

Fernandes-Rosa, F. L.

Publications and source records attributed to Fernandes-Rosa, F. L..

3 recordsLinked to original sources

bioRxiv2025

Sexually dimorphic interzonal crosstalk reshapes the adrenal cortex in response to pathophysiological challenges

Structured Abstract BackgroundPrimary aldosteronism is the most common form of secondary arterial hypertension, due to autonomous aldosterone production from the adrenal cortex. Genome wide association studies discovered genetic risk loci associated with the disease, which may affect adrenal cortex renewal, differentiation and lineage conversion. Genetic susceptibility may be modulated by environmental challenges. MethodHere we investigate how environmental cues affecting mineralocorticoid output modulate adrenal cortex homeostasis, cell lineage conversion and zone-specific transcriptional landscape. We have used a newly developed Cyp11b2Cremouse model and characterised its adaptation to a high or low salt diet (HSD, LSD), as well as dexamethasone (DEX) treatment. To explore underlying mechanisms, deep functional and morphological phenotyping, lineage tracing and spatial transcriptomics of the adrenal cortex were performed. ResultsCyp11b2 expression was detected in Cyp11b2Cre-mTmG mice as early as day P1 in different areas of the ZG, associated with high plasma aldosterone levels, with lineage conversion of zona glomerulosa (ZG) into zona fasciculata (ZF) cells progressing between 2 and 9 weeks of age and a progressive reduction of ZG size and evolution of cell components of the adrenal cortex over time. Transdifferentiation progressed into the X-zone (ZX) in females, revealing a previously unrecognized connection between ZF and ZX cells in adult mice. A sexually dimorphic, reciprocal interaction between the ZG and the ZF in adapting to salt diets or DEX treatment was observed, involving changes in cell composition and transcriptional reprogramming of the three zones. ConclusionHSD, LSD and DEX induce a sexually dimorphic cellular and transcriptional response, involving all layers of the adrenal cortex, and the reciprocal contribution of ZG and ZF cells, indicating functional interaction between adrenocortical zones in adapting to external cues.

physiology↗

bioRxiv2025

Regulation of Aldosterone Secretion by Substance P and the NK1 Receptor in Aldosterone-Producing Adenomas

Aldosterone-producing adenoma (APA) is a major cause of primary aldosteronism (PA), the most frequent form of secondary hypertension. Although somatic mutations in ion channels within APA have been shown to activate Ca2+ signaling and drive aldosterone production, the pathophysiology of PA remains partially understood. Substance P (SP), encoded by the TAC1 gene, is a neuropeptide of the tachykinin family, known for its role in stimulating aldosterone production through activation of the neurokinin 1 receptor (NK1R) in the human adrenal cortex. The aim of our work was to investigate the presence of SP nerve fibers and the NK1 receptor in a large series of APA to assess the potential role of this neuropeptide in the pathophysiology of PA. We analyzed 56 APA tissues using molecular, immunohistochemical, and functional techniques to assess the expression of SP and NK1R and examine the action of SP on aldosterone secretion. SP-positive nerve fibers were detected in 90% of the APA tissues, appearing localized both within and around the adenomas, which also showed strong NK1R expression. Functional studies revealed that SP stimulated aldosterone secretion in 6 of 10 APA cultures. The NK1R antagonist aprepitant inhibited SP-induced aldosterone secretion in 3 of the 4 SP-responsive APA cultures on which the antagonist was tested. Additionally, in perifused APA explants, SP influenced aldosterone pulsatility, resulting in enhanced mineralocorticoid secretion. These findings suggest that the SP-NK1R signaling pathway may contribute to APA pathophysiology and represent a novel potential target for the pharmacological treatment of PA in a subset of patients.

physiology↗

bioRxiv2024

Modulation of calcium signaling on demand to decipher the molecular mechanisms of primary aldosteronism

Primary aldosteronism (PA) is the most common form of secondary hypertension. Major advances have been made in our understanding of PA with the identification of germline and somatic mutations in ion pumps and channels. These mutations lead to the activation of calcium signalling, the major trigger of aldosterone biosynthesis. To elucidate the molecular mechanisms underlying the development of PA, we established an adrenocortical cell model in which we can modulate sodium entry into the cells "on demand" leading to calcium signalling activation. These cells recapitulated the major features of KCNJ5 mutations, the most frequent genetic alteration identified in Aldosterone-Producing-Adenoma. Activation of calcium signalling was associated with increased aldosterone biosynthesis and decreased cell proliferation. RNA sequencing and steroidome analyses revealed unique profiles associated with Na+ entry. Altogether, this work offers valuable insights into the role of sodium-induced calcium signalling in PA development and paves the way for developing new therapeutic strategies.

physiology↗