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Emenecker, R.

Publications and source records attributed to Emenecker, R..

2 recordsLinked to original sources

Progress toward the identification and stacking of crucial domestication traits in pennycress

The oilseed species Thlaspi arvense (pennycress) is being domesticated as a new crop that can provide both important ecosystem services and intensify farmland output. Through the use of high throughput sequencing and phenotyping, along with classical mutagenesis key traits needed for pennycress domestication have been identified. Domestication traits identified herein include reduced pod shatter, early maturity, reduced seed glucosinolate levels, and improved oil fatty acid content. By taking advantage of pennycress close genetic relationship with Arabidopsis thaliana, the causative mutations responsible for each of these traits have been identified. These mutations have been used to develop molecular markers to begin to stack the traits into individual lines.

plant biology

Mechanism of microtubule plus-end tracking by the plant-specific SPR1 protein and its development as a versatile plus-end marker

The dynamics and functions of microtubule plus-ends are governed by microtubule plus-end tracking proteins (+TIPs). Here, we report that the diminutive Arabidopsis thaliana SPIRAL1 (SPR1) protein, which regulates directional cell expansion, is an autonomous +TIP. Using in vitro reconstitution experiments and total internal reflection fluorescence microscopy, we demonstrate that the conserved N-terminal domain of SPR1 and its GGG motif are necessary for +TIP activity, whereas the conserved C-terminal domain and its PGGG motif are not. In addition, we show that the N-and C-terminal domains, either separated or in tandem, are sufficient for +TIP activity and do not significantly perturb microtubule plus-end dynamics compared to full-length SPR1. We also found that exogenously expressed SPR1-GFP and NC-GFP label microtubule plus-ends in animal cells. These data establish SPR1 as a new type of intrinsic +TIP and demonstrate its utility as a universal microtubule plus-end marker.

cell biology