Emelyantsev, S. | bioRxiv Science

bioRxiv2023

SOLUBILIZER OF BACTERIAL ORIGIN SURFACTIN INCREASES THE BIOLOGICAL ACTIVITY OF C60 FULLERENE

Currently, there exists conflicting data regarding the biological activity of unmodified fullerene C60. Various sources report its toxicity, geroprotective activity, and potential interaction with DNA. Contradictory findings regarding the toxicity of C60 may arise from the use of toxic solvents, as well as the influence of bioavailability and bioactivity on the preparation conditions of C60 suspensions. Furthermore, the microbiota of experimental animals can impact geroprotective activity results by releasing surfactants that facilitate substance penetration through the cell membrane. In this study, we selected conditions for solubilizing fullerene C60 in a solution of surfactin, a surfactant of bacterial origin, as well as in a 2% aqueous solution of TWEEN 80, employing ultrasound. Through bioluminescent analysis using lux biosensors in E. coli MG1655, we observed that C60 in surfactin reduced induced genotoxic and oxidative stress. Given that surfactin enhances membrane permeability to fullerene C60, suspensions of fullerene in designated concentrations of surfactin can be regarded as a DNA protector and antioxidant, warranting further investigation as a promising component of novel drugs.

genetics↗

bioRxiv2023

Probiotic bacteria Lacticaseibacillus rhamnosus L108 and Lacticaseibacillus delbrueckii R2 increase lifespan and influence the expression of Caenorhabditis elegans longevity genes

AnnotationNumerous studies have shown that probiotics hold great promise in slowing down the aging process and extending life expectancy. Bacteria of the genus Lacticaseibacillus have been found to possess antioxidant, antimutagenic and anti-inflammatory effects, as well as the ability to regulate the expression of genes that control signaling defense mechanisms in model objects. In this study, we used the nematode Caenorhabditis elegans as a model organism to investigate the impact of Lacticaseibacillus rhamnosus L108(L.rhamnosus L108) and Lacticaseibacillus delbrueckii R2 on nematode lifespan and the expression levels of genes associated with healthy aging. Our results demonstrate that the Lacticaseibacillus rhamnosus L108 strain exhibits antioxidant properties and increases the average lifespan of nematodes by 15%. Lacticaseibacillus delbrueckii R2 also has a positive effect, extending the lifespan of the worms by 21.4%. Furthermore, analysis of longevity gene expression reveals a correlation between increased lifespan and activation of the insulin/insulin-like factor-1 pathway. Moreover, we observed a significant increase in the expression of the skn-1 gene, which encodes antioxidant proteins and enhances the antioxidant response. Our findings suggest that the expression of the skn-1 gene and the transcription factor SKN-1 are associated with activation of the p38 MAPK signaling pathway. Therefore, it seems that probiotic bacteria Lacticaseibacillus rhamnosus L108 and Lactobacillus delbrueckii R2 have a positive effect on lifespan due to increased expression of genes that underlie the regulation of conserved signaling pathways related to host defense.

molecular biology↗