bioRxiv ScienceSearch

Biology subjects

Elizabeth Sinclair

Publications and source records attributed to Elizabeth Sinclair.

2 recordsLinked to original sources

Modeling Joint Abundance of Multiple Species Using Dirichlet Process Random Effects

We present a method for modeling multiple species distributions simultaneously using Dirichlet Process random effects to cluster species into guilds. Guilds are ecological groups of species that behave or react similarly to some environmental conditions. By modeling latent guild structure, we capture the cross-correlations in abundance or occurrence of species over surveys. In addition, ecological information about the community structure is obtained as a byproduct of the model. By clustering species into similar functional groups, prediction uncertainty of community structure at additional sites is reduced over treating each species separately. The method is illustrated with a small simulation demonstration, as well as an analysis of a mesopelagic fish survey from the eastern Bering Sea near Alaska. The simulation data analysis shows that guild membership can be extracted as the differences between groups become larger and if guild differences are small the model naturally collapses all the species into a small number of guilds which increases predictive efficiency by reducing the number of parameters to that which is supported by the data.

Ecology

Cryopreservation of human mucosal leukocytes

BackgroundUnderstanding how leukocytes in the cervicovaginal and colorectal mucosae respond to pathogens, and how medical interventions affect these responses, is important for developing better tools to prevent HIV and other sexually transmitted infections. An effective cryopreservation protocol for these cells following their isolation will make studying them more feasible.\n\nMethods and findingsTo find an optimal cryopreservation protocol for mucosal mononuclear leukocytes, we compared cryopreservation media and procedures using human vaginal leukocytes and confirmed our results with endocervical and colorectal leukocytes. Specifically, we measured the recovery of viable vaginal T cells and macrophages after cryopreservation with different cryopreservation media and handling procedures. We found several cryopreservation media that led to recoveries above 75%. Limiting the number and volume of washes increased the fraction of cells recovered by 10-15%, possibly due to the small cell numbers in mucosal samples. We confirmed that our cryopreservation protocol also works well for both endocervical and colorectal leukocytes.\n\nCryopreserved leukocytes had slightly increased cytokine responses to antigenic stimulation relative to the same cells tested fresh. Additionally, we tested whether it is better to cryopreserve endocervical cells on the cytobrush or in suspension.\n\nConclusionsLeukocytes from cervicovaginal and colorectal tissues can be cryopreserved with good recovery of functional, viable cells using several different cryopreservation media. The number and volume of washes has an experimentally meaningful effect on the percentage of cells recovered. We provide a detailed, step-by-step protocol with best practices for cryopreservation of mucosal leukocytes.

Immunology