Biology subjects

Edison, E. S.

Publications and source records attributed to Edison, E. S..

4 recordsLinked to original sources

bioRxiv2024

Exploring the link between extended red blood cell parameters and platelet indices in voluntary blood donors

BackgroundAs regular blood donors are prone to iron deficiency, importance of extended red blood cell (eRBC) parameters in identifying donors with depleted iron stores was investigated. Thrombocytosis has been well documented in patients affected with IDA. Thus, significance of eRBC parameters in identifying iron deficiency associated thrombocytosis was also examined in this cohort. MethodsBlood samples were collected in EDTA tubes from consenting donors for analyses of routine haematological and eRBC parameters. Serum samples were isolated for estimation of iron parameters. ResultsIron deficient donors had significantly altered eRBC parameters. Among them, Ret-He with a cut-off of [≥]32 pg had high AUC (0.822) and showed relatively high sensitivity & specificity in detecting iron deficiency. Combination of Ret-He with CCI increased sensitivity & specificity to 90.6% and 98.2%, in detection of donors affected with iron restricted erythropoiesis. This cohort had increased platelet counts, which showed significant association with Ret-He ({beta}= -0.373), RBC-He ({beta}= -0.384), CCI ({beta}= 0.384), Hypo-He ({beta}=0.494) and Micro-R ({beta}= 0.299). Elevated platelet counts also showed significant correlations with these eRBC parameters, which was absent in iron replete donors. ConclusionseRBC parameters are sensitive indicators of non-anaemic iron deficiency, which may be enhanced by combining them. Their significant association with elevated platelet counts in iron deficient donors, highlights their importance in reflecting iron deficiency associated thrombocytosis. Impact StatementThe present study discusses utility of eRBC parameters in detecting non-anaemic iron deficiency, in regular voluntary blood donors. Previous reports have investigated the importance of these parameters in identifying IDA in blood donors. The present study is the first one which indicates combining different eRBC parameters such as Ret-He and CCI increases their accuracy of detection. They were also significantly associated with elevated platelet counts in iron deficient donors, which was absent in iron replete individuals. This link between eRBC parameters and higher platelet counts in healthy donors affected with non-anaemic iron deficiency has not been reported before.

bioinformatics↗

bioRxiv2024

Investigating the influence of iron parameters in regulating platelet recovery in Apheresis Platelet Donors

IntroductionThe present study investigates changes in haematological parameters in the donor during apheresis and following donation. Effect of iron parameters in influencing platelet recovery was studied. MethodsBlood samples were collected in EDTA tubes for analyses of haematological parameters. Serum samples were isolated for estimation of iron parameters. ResultsHb, RBC count and Hct increased during apheresis, but no significant changes were observed after 48 hrs post donation. Platelet recovery rates increased from 78 % after 48 hours to > 100% on day 10 (p < 0.001). Ferritin, serum iron & Transferrin saturation initially increased and subsequently declined from day 7 post donation, which may influence platelet recovery. Recovery rates were also higher in donors with lower iron stores. ConclusionsChanges in haematological parameters during plateletpheresis are transient and optimal time required for platelet recovery is dependent on pre-donation counts. Recovery of platelet counts may be positively regulated by donor iron parameters.

molecular biology↗

bioRxiv2024

Targeting Metabolic Dependencies in Acute Myeloid Leukemia: A Dual Strategy using Arsenic Trioxide and Artesunate.

Acute myeloid leukemia (AML) remains a difficult disease to cure despite recent advances. Off-target side effects of therapy, especially prolonged cytopenia, lead to significant morbidity and mortality. There is an increased recognition that in AML, there is a potentially vulnerable dependence on OXPHOS metabolism, more so in the leukemia stem cell compartment (AML-LSC) that could be exploited. Drug re-purposing screens have suggested the possible role of artesunate (ART) in inhibiting mitochondrial respiration and arsenic trioxide (ATO) in inhibiting glycolysis. Here, we explore the potential of using a combination of ART and ATO to treat AML. Through in-vitro and in-vivo studies, we demonstrate the potential of this combination along with hypo-methylating agents and other mitocans, such as BCL-2 inhibitor venetoclax, to be used in the treatment of AML with minimal off-target effect on normal hematopoietic stem cells (HSC). These observations warrant further exploration of such novel combinations in clinical trials.

cancer biology↗

bioRxiv2022

Impact of maternal iron deficiency anaemia on fetal iron status and placental iron transporters in human pregnancy

Iron deficiency anaemia is associated with maternal morbidity and poor pregnancy outcomes. Placenta expresses both haem and non-haem iron transport proteins. The aim of the study is to examine the expression of placental iron trafficking molecules and associate them with maternal and neonatal iron status. Pregnant women who received prenatal care at the department of community health and development, Christian Medical College, Vellore, India for childbirth were recruited between 2016-2018. Pregnant women who were 18-35 years old with gestational age (GA) of [≥]36weeks were eligible to participate in the study. In a prospective cohort of pregnant women, 22% were iron deficiency anaemia (IDA) and 42% were iron replete. Pregnant women in the different groups were mutually exclusive. Samples were collected (Maternal blood, placental tissue, and cord blood) from pregnant women with gestational age of [≥]38 weeks at the time of delivery. Mean gestational age at first visit and delivery was 12.8{+/-}2.72 weeks and 39{+/-}1.65 weeks, respectively. Hemoglobin (9.3{+/-}0.9g/dl) and ferritin (15.4(0.8-28.3) ng/ml) levels at delivery were significantly decreased in IDA as compared to other groups. The foetal haemoglobin and ferritin levels were in the normal range in all groups. We further analysed the expression of iron transport genes in the placenta in the iron replete controls and the IDA group. Under maternal iron insufficiency, the expression of placental iron transporters DMT1 and FPN1 were upregulated at the transcriptional level. There was no correlation of maternal and cord blood hepcidin with foetal iron status in IDA. Thus, placental iron traffickers respond to maternal iron deficiency by increasing their expression and allowing sufficient iron to pass to the foetus.

molecular biology↗