Biology subjects

Drozdova, M. Y.

Publications and source records attributed to Drozdova, M. Y..

3 recordsLinked to original sources

bioRxiv2022

The effect of trans-cinnamic acid isolated from the root cultures of the Baikal skullcap (Scutellaria baicalensis) on the life expectancy and survival of Caenorhabditis elegans

Oxidative stress is an increase in reactive oxygen species, which are signaling molecules of various pathologies in the body of living beings. Baikal skullcap (Scutellaria baicalensis) is a valuable source of biologically active substances with various pharmacological activity. The aim of the study is to study the effect of trans-cinnamic acid isolated from root cultures of Scutellaria baicalensis on the lifespan of a model organism of C. elegans, as well as its survival under conditions of oxidative and thermal stress. It was shown that trans-cinnamic acid increased the lifespan of nematodes, while the best concentration of phytomaterials was 50 microns. In addition, all tested concentrations (10-200) had a positive effect on nematodes under oxidative stress caused by paraquat. No positive effect of trans-cinnamic acid was detected during thermal exposure. In general, the results show the antioxidant potential of trans-cinnamic acid from plant material under oxidative stress, as well as the positive effect of the substance on the lifespan of C. elegans.

plant biology↗

bioRxiv2022

The effect of biohanin A isolated from the callus culture of meadow clover (Trifolium pratense L.) on the life expectancy and survival of Caenorhabditis elegans

Oxidative stress, in which healthy aging is limited, is a problem, since in the process there is an uncontrolled production of reactive radicals that negatively affect cells. Heat stress is closely related to oxidative stress, as it stimulates the production of radicals. Meadow clover (Trifolium pratense L.) is a promising source of isoflavonoids that have various positive effects on the body. The aim of the work is to study the effect of biohanin A, isolated from Trifolium pratense L. callus cultures, on the life expectancy of the C. elegans model organism, as well as its survival under oxidative and thermal stress. It was found that biohanin A increased the lifespan of C. elegans worms. The best concentration was determined - 100 microns, at which the survival rate of nematodes after 61 days of incubation was 16.0%. After 48 hours, the survival rate of worms was the highest (87.1%) at a concentration of biohanin A of 200 microns. Other concentrations 10, 50, 100 (81,2, 83,0, 82,4 %) they also showed a higher survival rate compared to the control (74.0%). In addition, the survival rate under heat stress of C. elegans was higher by 12.0; 8.4; 4.0% compared with the control. Thus, the results show the antioxidant potential of biohanin A from plant material under oxidative and thermal stress. The substance also had a positive effect on the lifespan of C. elegans.

plant biology↗

bioRxiv2021

Development of methods for purification of individual biological active substances obtained from extracts of Hedysarum neglectum

Hedysarum neglectum is a forage plant. Xanthone glycoside - mangiferin is extracted from this plant and used for medicine "Alpizarin". In addition to substances of xanthone nature (mangiferin and isomangiferin) Hedysarum neglectum contains sugars, vitamins and provitamins, tannins; in the underground part it contains oligomeric catechins, isoflavonoids, butylphenols, alkaloids, tannins, flavonoids, saponins, coumarins, carbohydrates, vitamin C. For selecting optimal schemes of fractionization of substances, it is necessary to resort to multi-stage schemes of group-wide (preliminary) isolation and preparative accumulation. In particular cases, it is necessary to take into account the presence of concomitant substances, as well as the effectiveness and selectivity of the sorption-chromatographic technologies used. According to the results of the studies, the use of silica gel and sefadex LH-20 for the isolation of a complex of flavonoids and gallic acid is the most effective method for the selection of the optimal variant of the preparative isolation of the total amount of BAS in mcg/ml. The results of the research allowed us to identify the target biologically active substances with a degree of extraction of at least 80%: - fractions of xanthones, flavonoids, gallic acid.

plant biology↗