Doucey, M.-A. | bioRxiv Science

bioRxiv2025

A combined computational and experimental approach to successfully predict the behavior of metastatic tumor-associated macrophages in humanbreast cancer

We identified in human breast cancer a scarce population of Tumor-Associated Monocytes (TAMs) endowed with a pro-metastatic activity and associated with reduced distant-metastasis free survival of patients. We developed a novel framework combining computational and experimental methodologies to dampen TAM pro-metastatic activity. Multi-modal experimental data from TAMs exposed in vitro to a series of perturbations were collected to build a Boolean dynamical network of TAMs. This framework successfully identified the biological pathways underlying TAM pro-metastatic activity and predicted potent pharmacological interventions that inhibited the pro-metastatic activity of TAMs isolated from patient tumors. This study showcases the power of integrating computational predictions with experimental validation in identifying new therapeutic avenues that can be extended to other cancer types.

systems biology↗

bioRxiv2020

Systemic inflammation causes microglial dysfunction with a mixed AD-like pathology.

BackgroundAlzheimers disease (AD) is the primary cause of cognitive deficit in elderly humans. Late-onset AD (LOAD) is sporadic, multifactorial, non-Mendelian accounting at present for 95% of the cases in contrast to the genetic form. Risk factors for sporadic AD include Gene: Environment interactions. There is increasing evidence that lifestyle and environmental stress such as infection and chronic inflammation are underlying culprits of neurodegenerative dementia. Dementias that share or mimic pathological processes of AD include cerebrovascular diseases, Lewy body disease, TDP-43 proteinopathy. To date, very few mouse models reproduce the pathophysiological progression of mixed-vascular-AD, while the majority of studies have employed transgenic animals reproducing the familial form. MethodsWe have re-engineered the Polyinosinic:polycytidylic acid (PolyI:C) sterile infection model in wildtype C57Bl6 mice to obtain chronic low-grade systemic inflammation. We have conducted a cross-sectional analysis of aging PolyI:C and Saline control mice (3 months, 6 months, 9 months and 16 months), taking the hippocampus as a reference brain region, based on its vulnerability, and compared the brain aging phenotype to AD progression in humans with mild AD, severe AD and Controls (CTL), parallely in Vascular dementia (VaD) patient specimens. ResultsWe found that PolyI:C mice display both peripheral and central inflammation with a peak at 6 months, associated with memory deficits. The hippocampus is characterized by a pronounced and progressive tauopathy. In PolyI:C brains, microglia undergo aging-dependent morphological rearrangements progressively adopting a phagocytic phenotype. Transcriptomic analysis reveals a profound change in gene expression over the course of aging, with a peak in differential expression at 9 months. We confirm that the proinflammatory marker Lcn2 is one of the genes with the strongest upregulation in PolyI:C mice upon aging. Validation in brains from patients with increasing severity of AD and VaD shows a reproducibility of some gene targets in vascular dementia specimens rather than AD ones, in which only GFAP is strongly increased at the severe stages. ConclusionsThe PolyI:C model of sterile infection demonstrates that peripheral chronic inflammation is sufficient to cause neuropathological processes resembling a mixed-VaD-AD phenotype, with progressive tau hyperphosphorylation, changes in microglia morphology, astrogliosis and gene reprogramming reflecting increased neuroinflammation, vascular remodeling and the loss of neuronal functionality seen to some extent in humans.

neuroscience↗